Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Munir, E.; Hattori, T.; Shimada, M.
    Purification and characterization of isocitrate lyase from the wood-destroying basidiomycete Fomitopsis palustris grown on glucose (2002), Arch. Biochem. Biophys., 399, 225-231.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.3.1 2-mercaptoethanol 41% increase of activity at 1 mM Fomitopsis palustris
4.1.3.1 dithiothreitol 65% increase of activity at 1 mM Fomitopsis palustris
4.1.3.1 glutathione 35% increase of activity at 1 mM Fomitopsis palustris
4.1.3.1 L-cysteine 45% increase of activity at 1 mM Fomitopsis palustris

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.1 4-chloromercuribenzoate complete inactivation at 0.001 mM Fomitopsis palustris
4.1.3.1 iodoacetate isocitrate protects Fomitopsis palustris
4.1.3.1 Itaconate 89% inhibition at 1 mM Fomitopsis palustris
4.1.3.1 oxalate 86% inhibition at 10 mM Fomitopsis palustris
4.1.3.1 oxaloacetate 89% inhibition at 10 mM Fomitopsis palustris
4.1.3.1 pyruvate 87% inhibition at 10 mM Fomitopsis palustris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.1 0.092
-
 Mg2+ pH 7 Fomitopsis palustris
4.1.3.1 1.6
-
 DL-isocitrate pH 7 Fomitopsis palustris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.1 Mg2+ requirement, optimal concentration: 2 mM, Km value: 0.092 at pH 7 Fomitopsis palustris
4.1.3.1 Mn2+ can replace Mg2+ with 24% of the activity of Mg2+ Fomitopsis palustris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.1 60000
-
 3 * 60000, SDS-PAGE Fomitopsis palustris
4.1.3.1 186000
-
 gel filtration Fomitopsis palustris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.1 isocitrate Fomitopsis palustris regulatory enzyme playing a crucial role in fungal growth succinate + glyoxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Fomitopsis palustris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.1 76fold Fomitopsis palustris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.1 30.3
-
-
 Fomitopsis palustris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 isocitrate
-
 Fomitopsis palustris succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate regulatory enzyme playing a crucial role in fungal growth Fomitopsis palustris succinate + glyoxylate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.1 trimer 3 * 60000, SDS-PAGE Fomitopsis palustris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.1 32
-
 DL-isocitrate pH 7 Fomitopsis palustris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.1 7
-
-
 Fomitopsis palustris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.3.1 5 8.5 50% activity at pH 5, 22% activity at pH 8.5, no activity at pH 9 Fomitopsis palustris

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.3.1 0.037
-
 oxalate pH 7 Fomitopsis palustris
4.1.3.1 0.068
-
 Itaconate pH 7 Fomitopsis palustris