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Literature summary extracted from

  • Lim, J.H.; Choi, J.; Kim, W.; Ahn, B.Y.; Han, Y.S.
    Mutational analyses of Aquifex pyrophilus DNA ligase define essential domains for self-adenylation and DNA binding activity (2001), Arch. Biochem. Biophys., 388, 253-260.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.2 full length and truncated enzymes expressed in Escherichia coli BL21 (DE3) Aquifex pyrophilus

Protein Variants

EC Number Protein Variants Comment Organism
6.5.1.2 DELTA1-525 no DNA-binding Aquifex pyrophilus
6.5.1.2 DELTA1-90 very low self adenylation activity, active in DNA-ligation, reduced DNA-binding Aquifex pyrophilus
6.5.1.2 DELTA1-90,363-719 no self adenylation Aquifex pyrophilus
6.5.1.2 DELTA1-90,487-719 very low self adenylation activity Aquifex pyrophilus
6.5.1.2 DELTA363-719 full self adenylation activity Aquifex pyrophilus
6.5.1.2 DELTA517-719 full self adenylation activity Aquifex pyrophilus
6.5.1.2 DELTA636-719 active in DNA-ligation Aquifex pyrophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.2 Zn2+ zinc binding motif, Cys437, Cys440, Cys455, Cys460 Aquifex pyrophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m Aquifex pyrophilus DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.2 Aquifex pyrophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.2 recombinant proteins Aquifex pyrophilus

Reaction

EC Number Reaction Comment Organism Reaction ID
6.5.1.2 ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide 3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond Aquifex pyrophilus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Aquifex pyrophilus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA Aquifex pyrophilus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.2 NAD+ required Aquifex pyrophilus