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Literature summary extracted from

  • Bohlmann, J.; Martin, D.; Oldham, N.J.; Gershenzon, J.
    Terpenoid secondary metabolism in Arabidopsis thaliana: cDNA cloning, characterization, and functional expression of a myrcene/(E)-beta-ocimene synthase (2000), Arch. Biochem. Biophys., 375, 261-269.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.15 expression in Escherichia coli Arabidopsis thaliana
4.2.3.106 expression in Escherichia coli Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.15 69278
-
 x * 69278, calculated Arabidopsis thaliana
4.2.3.106 69278
-
 x * 69278, calculated Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.15 geranyl diphosphate Arabidopsis thaliana
-
 myrcene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.15 Arabidopsis thaliana Q9ZUH4
-
-
4.2.3.15 Arabidopsis thaliana Q9ZUH4 the tandem arginine motif RRX8W is conserved in the deduced protein of TPS10; bifunctional myrcene/(E)-beta-ocimene synthase
-
4.2.3.106 Arabidopsis thaliana Q9ZUH4 member oft the TPSb subfamily of angiosperm monoterpene synthases
-
4.2.3.106 Arabidopsis thaliana Q9ZUH4 the tandem arginine motif RRX8W is conserved in the deduced protein of TPS10; bifunctional myrcene/(E)-beta-ocimene synthase
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.15 geranyl diphosphate
-
 Arabidopsis thaliana myrcene + diphosphate
-
 ?
4.2.3.15 geranyl diphosphate
-
 Arabidopsis thaliana myrcene + diphosphate 56% of total hydrocarbon product ?
4.2.3.15 geranyl diphosphate
-
 Arabidopsis thaliana myrcene + diphosphate products of Arabidopsis thaliana TPS10 are 56% beta-myrcene, 20% (E)-beta-ocimene, and less than 5% each of (+)-limonene, (-)-limonene, 2-carene, and tricyclene and an unknown monoterpene ?
4.2.3.15 geranyl diphosphate
-
 Arabidopsis thaliana (E)-beta-ocimene + diphosphate 20% of total hydrocarbon product ?
4.2.3.15 geranyl diphosphate
-
 Arabidopsis thaliana (+)-limonene + diphosphate less than 5% of total hydrocarbon product ?
4.2.3.15 geranyl diphosphate
-
 Arabidopsis thaliana (-)-limonene + diphosphate less than 5% of total hydrocarbon product ?
4.2.3.15 geranyl diphosphate
-
 Arabidopsis thaliana 2-carene + diphosphate less than 5% of total hydrocarbon product ?
4.2.3.15 geranyl diphosphate
-
 Arabidopsis thaliana tricyclene + diphosphate less than 5% of total hydrocarbon product ?
4.2.3.106 geranyl diphosphate
-
 Arabidopsis thaliana (E)-beta-ocimene + diphosphate products of Arabidopsis thaliana TPS10 are 56% beta-myrcene, 20% (E)-beta-ocimene, and less than 5% each of (+)-limonene, (-)-limonene, 2-carene, and tricyclene and an unknown monoterpene ?
4.2.3.106 geranyl diphosphate
-
 Arabidopsis thaliana (E)-beta-ocimene + diphosphate main products are 56% beta-myrcene and 20% (E)-beta-ocimene plus minor amounts of (-)-limonene, (+)-limnonene, 2-carene and tricyclene ?

Subunits

EC Number Subunits Comment Organism
4.2.3.15 ? x * 69278, calculated Arabidopsis thaliana
4.2.3.106 ? x * 69278, calculated Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
4.2.3.15 AtTPS10
-
 Arabidopsis thaliana
4.2.3.15 myrcene/(E)-beta-ocimene synthase
-
 Arabidopsis thaliana
4.2.3.15 TPS10
-
 Arabidopsis thaliana
4.2.3.106 myrcene/(E)-beta-ocimene synthase
-
 Arabidopsis thaliana
4.2.3.106 TPS10
-
 Arabidopsis thaliana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.3.15 Arabidopsis thaliana calculated
-
 6
4.2.3.106 Arabidopsis thaliana calculated
-
 6