Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Honzatko, R.B.; Fromm, H.J.
    Structure-function studies of adenylosuccinate synthetase from Escherichia coli (1999), Arch. Biochem. Biophys., 370, 1-8.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.4.4
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.4 H41N site-directed mutagenesis Escherichia coli
6.3.4.4 K16Q site-directed mutagenesis Escherichia coli
6.3.4.4 N38A site-directed mutagenesis Escherichia coli
6.3.4.4 R143L site-directed mutagenesis Escherichia coli
6.3.4.4 R303L site-directed mutagenesis Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.4 Hadacidin
-
 Escherichia coli
6.3.4.4 succinate poor inhibitor Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.4 Mg2+ active site Mg2+ Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.4 GTP + IMP + L-aspartate Escherichia coli
-
 GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate Escherichia coli first committed step in the de novo biosynthesis of AMP, thermodynamically coupling the hydrolysis of GTP to the formation of adenylosuccinate from L-aspartate and IMP GDP + phosphate + adenylosuccinate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.4 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.4 GTP + IMP + L-aspartate
-
 Escherichia coli GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of AMP, thermodynamically coupling the hydrolysis of GTP to the formation of adenylosuccinate from L-aspartate and IMP Escherichia coli GDP + phosphate + adenylosuccinate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.4 dimer
-
 Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
6.3.4.4 adenosylsuccinate synthetase
-
 Escherichia coli
6.3.4.4 Adenylosuccinate synthetase
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.4 7.7
-
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.4.4 0.01
-
 Hadacidin
-
 Escherichia coli
6.3.4.4 1
-
 succinate
-
 Escherichia coli