Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sood, S.M.; Wu, M.X.; Hill, K.A.; Slattery, C.W.
    Characterization of zinc-depleted alanyl-tRNA synthetase from Escherichia coli: role of zinc (1999), Arch. Biochem. Biophys., 368, 380-384.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.1.1.7 Zn2+ stabilizes the enzyme Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.7 Urea denaturation at 3.3 M, the Zn2+-depleted enzyme is much more sensitive to denaturation by urea than the native enzyme Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.7 Zn2+ removal of Zn2+ leads to over 50% reduced activity, required for conformational stability and flexibility important for the enzymes activity Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.7 179000
-
 Zn2+-depleted enzyme, sedimentation coefficient and analytical ultracentrifugation Escherichia coli
6.1.1.7 186000
-
 native enzyme, sedimentation coefficient and analytical ultracentrifugation Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.7 ATP + L-alanine + tRNAAla Escherichia coli
-
 AMP + diphosphate + L-alanyl-tRNAAla
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.7 Escherichia coli
-
 recombinant wild-type from overexpression in Escherichia coli
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.7 ATP + L-alanine + tRNAAla
-
 Escherichia coli AMP + diphosphate + L-alanyl-tRNAAla
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.7 dimer
-
 Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
6.1.1.7 Ala-tRNA synthetase
-
 Escherichia coli
6.1.1.7 Alanine transfer RNA synthetase
-
 Escherichia coli
6.1.1.7 Alanine translase
-
 Escherichia coli
6.1.1.7 Alanine tRNA synthetase
-
 Escherichia coli
6.1.1.7 Alanine-transfer RNA ligase
-
 Escherichia coli
6.1.1.7 alanine-tRNA ligase
-
 Escherichia coli
6.1.1.7 Alanyl-transfer ribonucleate synthetase
-
 Escherichia coli
6.1.1.7 Alanyl-transfer ribonucleic acid synthetase
-
 Escherichia coli
6.1.1.7 Alanyl-transfer RNA synthetase
-
 Escherichia coli
6.1.1.7 Alanyl-tRNA synthetase
-
 Escherichia coli
6.1.1.7 AlaRS
-
 Escherichia coli
6.1.1.7 Synthase, alanyl-transfer ribonucleate
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.7 additional information
-
 the Zn2+-depleted enzyme shows reduced viscosity at 10°C compared to 37°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.7 7.5
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.7 ATP
-
 Escherichia coli