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Literature summary extracted from

  • Alkorta, I.; Park, C.; Kong, J.; Garbisu, C.; Alberti, M.; Pon, N.; Hearst, J.E.
    Rhodobacter capsulatus DNA topoisomerase I purification and characterization (1999), Arch. Biochem. Biophys., 362, 123-130.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.2.1 actinomycin D 10 mg/l, complete inhibition Rhodobacter capsulatus
5.6.2.1 ATP 5 mM, 75% inhibition, ccomplete inhibition at 10 mM, regardless of Mg2+ concentration Rhodobacter capsulatus
5.6.2.1 Ethidium bromide 0.25 mg/l, complete inhibition Rhodobacter capsulatus
5.6.2.1 heparin 1 mg/ml, complete inhibition Rhodobacter capsulatus
5.6.2.1 Hoechst 33258 5 mg/l, 70% inhibition Rhodobacter capsulatus
5.6.2.1 K+ above 150 mM Rhodobacter capsulatus
5.6.2.1 M13 DNA strong inhibitor of relaxing activity Rhodobacter capsulatus
5.6.2.1 Na+ above 150 mM Rhodobacter capsulatus
5.6.2.1 NEM 2 mM, complete inhibition Rhodobacter capsulatus
5.6.2.1 spermidine 4 mM, complete inhibition Rhodobacter capsulatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.2.1 Ca2+ optimal concentration is 4-8 mM, can replace the effect of Mg2+ Rhodobacter capsulatus
5.6.2.1 K+ stimulates below 60 mM Rhodobacter capsulatus
5.6.2.1 Mg2+ 2-8 mM, absolute requirement for divalent cation Rhodobacter capsulatus
5.6.2.1 Mn2+ optimal concentration is 2-8 mM, can replace the effect of Mg2+ Rhodobacter capsulatus
5.6.2.1 Na+ stimulates below 60 mM Rhodobacter capsulatus

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.1 Rhodobacter capsulatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.2.1
-
 Rhodobacter capsulatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.6.2.1 additional information
-
-
 Rhodobacter capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.1 negatively supercoiled PUC18 DNA unable to relax positively supercoiled DNA Rhodobacter capsulatus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.6.2.1 45
-
-
 Rhodobacter capsulatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.6.2.1 15
-
 180 min, stable Rhodobacter capsulatus
5.6.2.1 25
-
 180 min, about 80% loss of activity. 60 min, about 50% loss of activity Rhodobacter capsulatus
5.6.2.1 37
-
 180 min, complete loss of activity. 60 min, about 65% loss of activity Rhodobacter capsulatus
5.6.2.1 45
-
 60 min, 90% loss of activity Rhodobacter capsulatus
5.6.2.1 55
-
 15 min, complete inactivation Rhodobacter capsulatus