Any feedback?
Literature summary extracted from
- Possible roles for His 208 in the active-site region of chloroplast carbonic anhydrase from Pisum sativum (1999), Arch. Biochem. Biophys., 361, 17-24.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.1 | expressed in Escherichia coli BL21(DE3) | Pisum sativum |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.2.1.1 | H208A | site-directed mutagenesis, mutant is more easily inactivated by oxidation than the wild-type enzyme | Pisum sativum |
| 4.2.1.1 | H208A/C272A | site-directed mutagenesis | Pisum sativum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.1 | acetazolamide | - |
Pisum sativum |  |
| 4.2.1.1 | Cl- | - |
Pisum sativum | |
| 4.2.1.1 | SCN- | - |
Pisum sativum | |
| 4.2.1.1 | SO42- | - |
Pisum sativum | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.2.1.1 | chloroplast | - |
Pisum sativum | 9507 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.1 | Zn2+ | zinc-containing enzyme | Pisum sativum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.1 | 24200 | - |
SDS-PAGE | Pisum sativum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.1 | H2CO3 | Pisum sativum | - |
CO2 + H2O | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.1 | Pisum sativum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.1 | - |
Pisum sativum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.2.1.1 | cotyledon | - |
Pisum sativum | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.1 | H2CO3 | - |
Pisum sativum | CO2 + H2O | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.1 | carbonic anhydrase | - |
Pisum sativum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.1 | 0.05 | - |
CO2 | pH 6.2, 25°C, mutant H208A | Pisum sativum | |
| 4.2.1.1 | 0.135 | - |
CO2 | pH 6.2, 25°C, wild-type | Pisum sativum | |
| 4.2.1.1 | 0.28 | - |
CO2 | pH 7.3, 25°C, mutant H208A | Pisum sativum | |
| 4.2.1.1 | 0.31 | - |
CO2 | pH 7.3, 25°C, wild-type | Pisum sativum | |
| 4.2.1.1 | 0.74 | - |
CO2 | pH 8.8, 25°C, wild-type | Pisum sativum | |
| 4.2.1.1 | 1.36 | - |
CO2 | pH 8.8, 25°C, mutant H208A | Pisum sativum | |
| 4.2.1.1 | 6.08 | - |
CO2 | pH 8.8, 25°C, mutant H208A | Pisum sativum | |
| 4.2.1.1 | 6.08 | - |
CO2 | pH 8.8, 25°C, wild-type | Pisum sativum | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
