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Literature summary extracted from

  • Sajidan, A.; Farouk, A.; Greiner, R.; Jungblut, P.; Muller, E.C.; Borriss, R.
    Molecular and physiological characterisation of a 3-phytase from soil bacterium Klebsiella sp. ASR1 (2004), Appl. Microbiol. Biotechnol., 65, 110-117.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 expression in Escherichia coli Klebsiella sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 42000
-
 x * 42000, SDS-PAGE Klebsiella sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Klebsiella sp.
-
 ASR1
-
3.1.3.8 Klebsiella sp. ASR1
-
 ASR1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8
-
 Klebsiella sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 169
-
-
 Klebsiella sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 1-naphthyl phosphate + H2O 2.1% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. 1-naphthol + phosphate
-
 ?
3.1.3.8 2-glycerophosphate + H2O 19.8% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. glycerate + phosphate
-
 ?
3.1.3.8 2-naphthyl phosphate + H2O 4.9% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. 2-naphthol + phosphate
-
 ?
3.1.3.8 4-nitrophenyl phosphate + H2O 23.7% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. 4-nitrophenol + phosphate
-
 ?
3.1.3.8 ADP + H2O 7.5% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. ?
-
 ?
3.1.3.8 ADP + H2O 7.5% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. ASR1 ?
-
 ?
3.1.3.8 AMP + H2O 9.6% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. adenosine + phosphate
-
 ?
3.1.3.8 ATP + H2O 5.6% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. ?
-
 ?
3.1.3.8 D-fructose 6-phosphate + H2O 8.1% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. D-fructose + phosphate
-
 ?
3.1.3.8 D-fructose 6-phosphate + H2O 8.1% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. ASR1 D-fructose + phosphate
-
 ?
3.1.3.8 fructose 1,6-diphosphate + H2O 12.3% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. ?
-
 ?
3.1.3.8 fructose 1,6-diphosphate + H2O 12.3% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. ASR1 ?
-
 ?
3.1.3.8 Glucose 6-phosphate + H2O 2.7% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. Glucose + phosphate
-
 ?
3.1.3.8 Glucose 6-phosphate + H2O 2.7% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. ASR1 Glucose + phosphate
-
 ?
3.1.3.8 additional information no activity with GTP and diphosphate Klebsiella sp. ?
-
 ?
3.1.3.8 additional information no activity with GTP and diphosphate Klebsiella sp. ASR1 ?
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O step-wise dephosphorylation occurs via 1. D-myo-inositol 1,2,4,5,6-pentakisphosphate, 2. D-myo-inositol 1,2,5,6-tetrakisphosphate or D-myo-inositol 2,4,5,6-tetrakisphosphate, 3. D-myo-inositol 1,2,6-trisphosphate, D-myo-inositol 1,2,3-trisphosphate or D-myo-inositol 1,4,5-trisphosphate, 4. myo-inositol 1,2-bisphosphate or myo-inositol 2,5-bisphosphate, myo-inositol 4,5-bisphosphate or myo-inositol 2,4-bisphosphate. Myo-inositol 2-monophosphate is the final product Klebsiella sp. D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?
3.1.3.8 NADP+ + H2O 58.1% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. NAD+ + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 ? x * 42000, SDS-PAGE Klebsiella sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 PhyK
-
 Klebsiella sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 45
-
-
 Klebsiella sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 45
-
 15 min, stable below Klebsiella sp.
3.1.3.8 65
-
 15 min, complete inactivation Klebsiella sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 5
-
-
 Klebsiella sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 4 7 enzyme is virtually inactive below pH 4.0 and above pH 7.0 Klebsiella sp.