BRENDA - Enzyme Database

Purification and partial characterization of the Pyrococcus horikoshii methylmalonyl-CoA epimerase

Bobik, T.A.; Rasche, M.E.; Appl. Microbiol. Biotechnol. 63, 682-685 (2004)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
5.1.99.1
high level expression in Escherichia coli
Pyrococcus horikoshii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.1.99.1
0.079
-
methylmalonyl-CoA
-
Pyrococcus horikoshii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.1.99.1
31700
-
2 * 31700
Pyrococcus horikoshii
Organism
EC Number
Organism
UniProt
Commentary
Textmining
5.1.99.1
Pyrococcus horikoshii
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
5.1.99.1
-
Pyrococcus horikoshii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5.1.99.1
(R)-methylmalonyl-CoA
-
649408
Pyrococcus horikoshii
(S)-methylmalonyl-CoA
-
649408
Pyrococcus horikoshii
?
5.1.99.1
methylmalonyl-CoA
-
649408
Pyrococcus horikoshii
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
5.1.99.1
dimer
2 * 31700
Pyrococcus horikoshii
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
5.1.99.1
100
-
60 min, stable
Pyrococcus horikoshii
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.1.99.1
240
-
methylmalonyl-CoA
-
Pyrococcus horikoshii
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.1.99.1
high level expression in Escherichia coli
Pyrococcus horikoshii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.1.99.1
0.079
-
methylmalonyl-CoA
-
Pyrococcus horikoshii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.1.99.1
31700
-
2 * 31700
Pyrococcus horikoshii
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.1.99.1
-
Pyrococcus horikoshii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5.1.99.1
(R)-methylmalonyl-CoA
-
649408
Pyrococcus horikoshii
(S)-methylmalonyl-CoA
-
649408
Pyrococcus horikoshii
?
5.1.99.1
methylmalonyl-CoA
-
649408
Pyrococcus horikoshii
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.1.99.1
dimer
2 * 31700
Pyrococcus horikoshii
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
5.1.99.1
100
-
60 min, stable
Pyrococcus horikoshii
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.1.99.1
240
-
methylmalonyl-CoA
-
Pyrococcus horikoshii