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Literature summary extracted from
- A novel enzyme, D-3-hydroxyaspartate aldolase from Paracoccus denitrificans IFO 13301: purification, characterization, and gene cloning (2003), Appl. Microbiol. Biotechnol., 62, 53-60.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.3.14 | - |
Paracoccus denitrificans |
| 4.1.3.41 | expressed in Escherichia coli XL1-Blue MRF cells | Paracoccus denitrificans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.3.41 | EDTA | 82% inhibition at 1 mM | Paracoccus denitrificans |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.3.41 | 0.4 | - |
D-erythro-3-hydroxyaspartate | in 0.02 mM HEPES buffer, pH 8.0, at 30°C | Paracoccus denitrificans | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.3.41 | Co2+ | 2.0fold increase of specific activity in the presence of 1 mM Co2+ | Paracoccus denitrificans | |
| 4.1.3.41 | Mg2+ | 5.4fold increase of specific activity in the presence of 1 mM Mg2+ | Paracoccus denitrificans | |
| 4.1.3.41 | Mn2+ | 8.1fold increase of specific activity in the presence of 1 mM Mn2+ | Paracoccus denitrificans | |
| 4.1.3.41 | additional information | K+ and Na+ do not have an effect on the enzymatic activity | Paracoccus denitrificans |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.41 | 41633 | - |
2 * 41633, calculated from amino acid sequence | Paracoccus denitrificans |
| 4.1.3.41 | 43000 | - |
2 * 43000, SDS-PAGE | Paracoccus denitrificans |
| 4.1.3.41 | 80000 | - |
gel filtration | Paracoccus denitrificans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.3.14 | Paracoccus denitrificans | - |
IFO13301 | - |
| 4.1.3.14 | Paracoccus denitrificans IFO13301 | - |
IFO13301 | - |
| 4.1.3.41 | Paracoccus denitrificans | Q8GRC8 | strain IFO 13301 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.3.14 | homogeneity | Paracoccus denitrificans |
| 4.1.3.41 | ammonium sulfate fractionation, hydroxyapatite column chromatography, DEAE-Toyopearl column chromatography, phenyl-Toyopearl column chromatography, Superdex 200 gel filtration, and Mono Q column chromatography | Paracoccus denitrificans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.41 | 0.36 | - |
after 50fold purification, using DL-threo-3-hydroxyaspartate as substrate, in 0.02 mM HEPES buffer, pH 8.0, at 30°C | Paracoccus denitrificans |
| 4.1.3.41 | 0.6 | - |
crude extract, in 0.02 mM HEPES buffer, pH 8.0, at 30°C | Paracoccus denitrificans |
| 4.1.3.41 | 14.6 | - |
after 50fold purification, using D-threo-3-3,4-methylenedioxyphenylserine as substrate, in 0.02 mM HEPES buffer, pH 8.0, at 30°C | Paracoccus denitrificans |
| 4.1.3.41 | 17.5 | - |
after 50fold purification, using D-erythro-3-3,4-methylenedioxyphenylserine as substrate, in 0.02 mM HEPES buffer, pH 8.0, at 30°C | Paracoccus denitrificans |
| 4.1.3.41 | 20 | - |
after 50fold purification, using D-threonine as substrate, in 0.02 mM HEPES buffer, pH 8.0, at 30°C | Paracoccus denitrificans |
| 4.1.3.41 | 25 | - |
after 50fold purification, using D-allo-threonine as substrate, in 0.02 mM HEPES buffer, pH 8.0, at 30°C | Paracoccus denitrificans |
| 4.1.3.41 | 30 | - |
after 50fold purification, using D-erythro-3-hydroxyaspartate as substrate, in 0.02 mM HEPES buffer, pH 8.0, at 30°C | Paracoccus denitrificans |
| 4.1.3.41 | 95 | - |
after 50fold purification, using D-threo-3-phenylserine as substrate, in 0.02 mM HEPES buffer, pH 8.0, at 30°C | Paracoccus denitrificans |
| 4.1.3.41 | 99 | - |
after 50fold purification, using D-erythro-3-phenylserine as substrate, in 0.02 mM HEPES buffer, pH 8.0, at 30°C | Paracoccus denitrificans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.3.14 | D-3-3,4-dihydroxyphenylserine | - |
Paracoccus denitrificans | ? | - |
? | |
| 4.1.3.14 | D-3-3,4-dihydroxyphenylserine | - |
Paracoccus denitrificans IFO13301 | ? | - |
? | |
| 4.1.3.14 | D-3-3,4-methylenedioxyphenylserine | - |
Paracoccus denitrificans | ? | - |
? | |
| 4.1.3.14 | D-3-3,4-methylenedioxyphenylserine | - |
Paracoccus denitrificans IFO13301 | ? | - |
? | |
| 4.1.3.14 | D-3-hydroxyaspartate | enzyme is strictly D-specific to the alpha-position, but does not distinguish between threo and erythro forms at the beta-position | Paracoccus denitrificans | glycine + glyoxylate | - |
? | |
| 4.1.3.14 | D-3-hydroxyaspartate | enzyme is strictly D-specific to the alpha-position, but does not distinguish between threo and erythro forms at the beta-position | Paracoccus denitrificans IFO13301 | glycine + glyoxylate | - |
? | |
| 4.1.3.14 | D-3-phenylserine | - |
Paracoccus denitrificans | ? | - |
? | |
| 4.1.3.14 | D-3-phenylserine | - |
Paracoccus denitrificans IFO13301 | ? | - |
? | |
| 4.1.3.14 | D-threonine | - |
Paracoccus denitrificans | ? | - |
? | |
| 4.1.3.14 | D-threonine | - |
Paracoccus denitrificans IFO13301 | ? | - |
? | |
| 4.1.3.41 | 2-amino-3-hydroxybutanedioic acid | - |
Paracoccus denitrificans | aminoacetic acid + oxoacetic acid | - |
r | |
| 4.1.3.41 | allo-threonine | - |
Paracoccus denitrificans | ? | - |
? | |
| 4.1.3.41 | D-3-3,4-dihydroxyphenylserine | - |
Paracoccus denitrificans | ? | - |
? | |
| 4.1.3.41 | D-erythro-3-3,4-methylenedioxyphenylserine | - |
Paracoccus denitrificans | ? | - |
? | |
| 4.1.3.41 | D-erythro-3-hydroxyaspartate | the enzyme is strictly D-specific as to the alpha-position, whereas it does not distinguish between threo and erythro forms at the beta-position | Paracoccus denitrificans | glycine + glyoxylate | - |
? | |
| 4.1.3.41 | D-erythro-3-phenylserine | - |
Paracoccus denitrificans | ? | - |
? | |
| 4.1.3.41 | D-threo-3-3,4-methylenedioxyphenylserine | - |
Paracoccus denitrificans | ? | - |
? | |
| 4.1.3.41 | D-threo-3-phenylserine | - |
Paracoccus denitrificans | ? | - |
? | |
| 4.1.3.41 | additional information | the enzyme shows no activity towards L-erythro-3-hydroxyaspartate, L-threo-3-hydroxyaspartate, L-threonine, L-allo-threonine, L-erythro-3-phenylserine, L-threo-3-phenylserine, L-erythro-3-3,4-methylenedioxyphenylserine, and L-threo-3-3,4-methylenedioxyphenylserine | Paracoccus denitrificans | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.3.41 | homodimer | 2 * 43000, SDS-PAGE | Paracoccus denitrificans |
| 4.1.3.41 | homodimer | 2 * 41633, calculated from amino acid sequence | Paracoccus denitrificans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.3.14 | d-HAA | - |
Paracoccus denitrificans |
| 4.1.3.41 | D-3-hydroxyaspartate aldolase | - |
Paracoccus denitrificans |
| 4.1.3.41 | d-HAA | - |
Paracoccus denitrificans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.41 | 35 | - |
- |
Paracoccus denitrificans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.41 | 45 | - |
the enzyme retains 50% activity upon heating at 45°C for 30 min | Paracoccus denitrificans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.41 | 9 | - |
- |
Paracoccus denitrificans |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.41 | 7.5 | 10 | - |
Paracoccus denitrificans |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.41 | 6.5 | 8.5 | the enzyme is stable between pH 6.5 and 8.5 for 30 min at 30°C | Paracoccus denitrificans |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.3.41 | pyridoxal 5'-phosphate | - |
Paracoccus denitrificans | |
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