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Literature summary extracted from

  • Kademi, A.; Ait-Abdelkader, N.; Fakhreddine, L.; Baratti, J.
    Purification and characterization of a thermostable esterase from the moderate thermophile Bacillus circulans (2000), Appl. Microbiol. Biotechnol., 54, 173-179.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.1 synthesis usage of the enzyme as biocatalyst for biotransformations in non-aqueous media in industrial applications at high and also at moderate temperatures Niallia circulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 diethyldicarbonate weak inhibition Niallia circulans
3.1.1.1 EDTA slight inhibition Niallia circulans
3.1.1.1 eserine complete inhibition Niallia circulans
3.1.1.1 HgCl2 complete inhibition Niallia circulans
3.1.1.1 p-hydroxymercuribenzoic acid complete inhibition Niallia circulans
3.1.1.1 phenylmethylsulfonyl fluoride complete inhibition Niallia circulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.2
-
4-nitrophenyl octanoate pH 8.0, 50°C Niallia circulans
3.1.1.1 1.5
-
4-nitrophenyl propionate pH 8.0, 50°C Niallia circulans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.1 additional information contains no metal Niallia circulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 30000
-
3 * 30000, SDS-PAGE Niallia circulans
3.1.1.1 95000
-
gel filtration Niallia circulans

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Niallia circulans
-
moderate thermophile, strain MAS2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1 711fold, to homogeneity Niallia circulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 88.2
-
purified enzyme Niallia circulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl butyrate + H2O
-
Niallia circulans 4-nitrophenol + butyrate
-
?
3.1.1.1 4-nitrophenyl hexanoate + H2O
-
Niallia circulans 4-nitrophenol + hexanoate
-
?
3.1.1.1 4-nitrophenyl laurate + H2O
-
Niallia circulans 4-nitrophenol + lauric acid
-
?
3.1.1.1 4-nitrophenyl myristate + H2O
-
Niallia circulans 4-nitrophenol + myristic acid
-
?
3.1.1.1 4-nitrophenyl octanoate + H2O
-
Niallia circulans 4-nitrophenol + octanoate
-
?
3.1.1.1 4-nitrophenyl palmitate + H2O
-
Niallia circulans 4-nitrophenol + palmitic acid
-
?
3.1.1.1 4-nitrophenyl propionate + H2O
-
Niallia circulans 4-nitrophenol + propionate
-
?
3.1.1.1 additional information maked specificity for mid-chain-length fatty acids of 3-8 C-atoms Niallia circulans ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 trimer 3 * 30000, SDS-PAGE Niallia circulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 55
-
-
Niallia circulans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.1 21 74 50% of maximal activity at 30-37°C, maximal activity at 55°C, 60% activity at 74°C Niallia circulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 60
-
completely stable up to Niallia circulans
3.1.1.1 71
-
50% inactivation after 1 h Niallia circulans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.1 51
-
4-nitrophenyl octanoate pH 8.0, 50°C Niallia circulans
3.1.1.1 144
-
4-nitrophenyl propionate pH 8.0, 50°C Niallia circulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 9 9.7 above Niallia circulans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.1 7 11
-
Niallia circulans