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Literature summary extracted from

  • Cybinski, D.H.; Layton, I.; Lowry, J.B.; Dalrymple, B.P.
    An acetylxylan esterase and a xylanase expressed from genes cloned from the ruminal fungus Neocallimastix patriciarum act synergistically to degrade acetylated xylans (1999), Appl. Microbiol. Biotechnol., 52, 221-225.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.72 gene bnaA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli as His-tagged protein Neocallimastix patriciarum

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.72 Neocallimastix patriciarum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.72 gene bnaA, recombinant His-tagged enzyme from Escherichia coli Neocallimastix patriciarum

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.72 acetylxylan + 3 H2O = 3 acetic acid + xylan catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, alpha-naphthyl acetate, p-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin. Neocallimastix patriciarum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.72 acetylated xylan + H2O substrate prepared from birchwood, the recombinant enzyme acts synergistically with xylanase to degrade acetylated xylan Neocallimastix patriciarum xylan + acetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.72 BnaA
-
 Neocallimastix patriciarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.72 39
-
 assay at Neocallimastix patriciarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.72 6
-
 assay at Neocallimastix patriciarum