Literature summary extracted from

Polderman-Tijmes, J.J.; Jekel, P.A.; de Vries, E.J.; van Merode, A.E.; Floris, R.; van der Laan, J.M.; Sonke, T.; Janssen, D.B.
Cloning, sequence analysis, and expression in Escherichia coli of the gene encoding an alpha-amino acid ester hydrolase from Acetobacter turbidans (2002), Appl. Environ. Microbiol., 68, 211-218.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.1.1.43	expression in Escherichia coli	Acetobacter pasteurianus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.1.1.43	0.34	-	cephalexin	30°C, pH 6.2	Acetobacter pasteurianus
3.1.1.43	1	-	ampicillin	30°C, pH 6.2	Acetobacter pasteurianus
3.1.1.43	1.1	-	D-2-nitro-5-[(phenylglycyl)amino]benzoic acid	30°C, pH 6.2	Acetobacter pasteurianus
3.1.1.43	1.7	-	cefadroxil	30°C, pH 6.2	Acetobacter pasteurianus
3.1.1.43	2.6	-	amoxicillin	30°C, pH 6.2	Acetobacter pasteurianus
3.1.1.43	7	-	D-phenylglycine methyl ester	30°C, pH 6.2	Acetobacter pasteurianus
3.1.1.43	13	-	D-phenylglycine amide	30°C, pH 6.2	Acetobacter pasteurianus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.1.1.43	70000	-	2 * 70000, SDS-PAGE	Acetobacter pasteurianus
3.1.1.43	150000	-	dynamic light scattering	Acetobacter pasteurianus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3.1.1.43	an alpha-aminoacid ester + H2O	Acetobacter pasteurianus	-	an alpha-amino acid + an alcohol	-	r

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.1.43	Acetobacter pasteurianus	Q8VRK8	ATCC 9325	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.1.1.43	CM-Sepharose, hydrophobic interaction, gel filtration	Acetobacter pasteurianus

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.1.1.43	667	-	Cephalexin synthesis	Acetobacter pasteurianus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.1.43	amoxicillin + H2O	-	Acetobacter pasteurianus	D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid	-	?
3.1.1.43	ampicillin + H2O	-	Acetobacter pasteurianus	(R)-2-phenylglycine + 6-aminopenicillanic acid	-	r
3.1.1.43	an alpha-aminoacid ester + H2O	-	Acetobacter pasteurianus	an alpha-amino acid + an alcohol	-	r
3.1.1.43	cefadroxil + H2O	-	Acetobacter pasteurianus	?	-	r
3.1.1.43	cephalexin + H2O	-	Acetobacter pasteurianus	D-phenylglycine + 7-aminodesacetoxycephalosporanic acid	-	r
3.1.1.43	D-2-nitro-5-[(phenylglycyl)amino]benzoic acid + H2O	-	Acetobacter pasteurianus	phenylglycine + p-nitrophenol	-	r
3.1.1.43	D-2-nitro-5-[(phenylglycyl)amino]benzoic acid + H2O	-	Acetobacter pasteurianus	?	-	?
3.1.1.43	D-phenylglycine amide + H2O	-	Acetobacter pasteurianus	D-phenylglycine + NH3	-	r
3.1.1.43	D-phenylglycine methyl ester + H2O	-	Acetobacter pasteurianus	D-phenylglycine + methanol	-	r

Subunits

EC Number	Subunits	Comment	Organism
3.1.1.43	dimer	2 * 70000, SDS-PAGE	Acetobacter pasteurianus

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.1.1.43	0.4	-	D-2-nitro-5-[(phenylglycyl)amino]benzoic acid	30°C, pH 6.2	Acetobacter pasteurianus
3.1.1.43	9.6	-	cefadroxil	30°C, pH 6.2	Acetobacter pasteurianus
3.1.1.43	10	-	amoxicillin	30°C, pH 6.2	Acetobacter pasteurianus
3.1.1.43	43	-	D-phenylglycine amide	30°C, pH 6.2	Acetobacter pasteurianus
3.1.1.43	162	-	ampicillin	30°C, pH 6.2	Acetobacter pasteurianus
3.1.1.43	347	-	cephalexin	30°C, pH 6.2	Acetobacter pasteurianus
3.1.1.43	1035	-	D-phenylglycine methyl ester	30°C, pH 6.2	Acetobacter pasteurianus
3.1.1.43	1040	-	D-phenylglycine methyl ester	30°C, pH 6.2	Acetobacter pasteurianus

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Release 2023.1 (January 2023)