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Literature summary extracted from

  • Da Costa, A.; Michaud, P.; Petit, E.; Heyraud, A.; Colin-Morel, P.; Courtois, B.; Courtois, J.
    Purification and properties of a glucuronan lyase from Sinorhizobium meliloti M5N1CS (NCIMB 40472) (2001), Appl. Environ. Microbiol., 67, 5197-5203.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.14 Ag+ 100% reduced activity at 1 mM Sinorhizobium meliloti
4.2.2.14 Ca2+ 21-30% reduced activity at 1 mM Sinorhizobium meliloti
4.2.2.14 Cd2+ 26-29% reduced activity at 1 mM Sinorhizobium meliloti
4.2.2.14 Cu2+ 85% reduced activity at 1 mM Sinorhizobium meliloti
4.2.2.14 EDTA 21-30% reduced activity at 1 mM Sinorhizobium meliloti
4.2.2.14 Hg2+ 94% reduced activity at 1 mM Sinorhizobium meliloti
4.2.2.14 Li+ 26-29% reduced activity at 1 mM Sinorhizobium meliloti
4.2.2.14 Mn2+ 21-30% reduced activity at 1 mM Sinorhizobium meliloti
4.2.2.14 additional information no inhibition by Mg2+ Sinorhizobium meliloti
4.2.2.14 NaN3 26-29% reduced activity at 1 mM Sinorhizobium meliloti
4.2.2.14 Zn2+ 59% reduced activity at 1 mM Sinorhizobium meliloti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.14 20000
-
 gel filtration Sinorhizobium meliloti

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.14 Sinorhizobium meliloti
-
 strain M5N1CS, NCIMB 40472
-
4.2.2.14 Sinorhizobium meliloti M5N1 CS
-
 strain M5N1CS, NCIMB 40472
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.14 to homogeneity, 394fold Sinorhizobium meliloti

Storage Stability

EC Number Storage Stability Organism
4.2.2.14 -80°C, 50 mM KCl, purified enzyme, stable for several weeks Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.14 3-O-acetylated glucuronan beta(1-4)-linked Sinorhizobium meliloti ?
-
 ?
4.2.2.14 3-O-acetylated glucuronan beta(1-4)-linked Sinorhizobium meliloti M5N1 CS ?
-
 ?
4.2.2.14 deacetylated polyglucuronate beta-(1-4)-linked, best substrate, enzyme shows specific endopolyglucuronate lyase activity Sinorhizobium meliloti 4,5-unsaturated oligoglucuronates
-
 ?
4.2.2.14 deacetylated polyglucuronate beta-(1-4)-linked, best substrate, enzyme shows specific endopolyglucuronate lyase activity Sinorhizobium meliloti M5N1 CS 4,5-unsaturated oligoglucuronates
-
 ?
4.2.2.14 additional information no activity with polyglucuronate methyl ester alpha-(1-4)-linked, hyaluronate beta-(1-3), beta(1-4)-linked, glucogluronan beta-(1-3), beta(1-4)-linked, alpha-L-guluronate blocks alpha-(1-4)-linked, beta-D-mannuronate blocks beta(1-4)-linked, 2,3-di-O-acetylated glucuronan beta(1-4)-linked, glucuronan beta(1-4)-linked Sinorhizobium meliloti ?
-
 ?
4.2.2.14 additional information no activity with polyglucuronate methyl ester alpha-(1-4)-linked, hyaluronate beta-(1-3), beta(1-4)-linked, glucogluronan beta-(1-3), beta(1-4)-linked, alpha-L-guluronate blocks alpha-(1-4)-linked, beta-D-mannuronate blocks beta(1-4)-linked, 2,3-di-O-acetylated glucuronan beta(1-4)-linked, glucuronan beta(1-4)-linked Sinorhizobium meliloti M5N1 CS ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.14 monomer 1 * 20000, SDS-PAGE Sinorhizobium meliloti

Synonyms

EC Number Synonyms Comment Organism
4.2.2.14 (1,4)-beta-D-glucuronan lyase
-
 Sinorhizobium meliloti
4.2.2.14 endopolyglucuronate lyase
-
 Sinorhizobium meliloti

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.14 50
-
-
 Sinorhizobium meliloti

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.14 52
-
 50% of maximal activity Sinorhizobium meliloti
4.2.2.14 67
-
 complete inactivation Sinorhizobium meliloti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.14 6.5
-
-
 Sinorhizobium meliloti

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.2.14 Sinorhizobium meliloti isoelectric focusing
-
 4.9