BRENDA - Enzyme Database

Inactivation of isocitrate lyase leads to increased production of medium-chain-length poly(3-hydroxyalkanoates) in Pseudomonas putida

Klinke, S.; Dauner, M.; Scott, G.; Kessler, B.; Witholt, B.; Appl. Environ. Microbiol. 66, 909-913 (2000)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Pseudomonas putida
rate-limiting enzyme of the citric acid cycle
succinate + glyoxylate
-
Pseudomonas putida
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Pseudomonas putida
-
wild-type: KT2442, mutant: KT217, poly(3-hydroxyalkanoate)-overproducing mutant in which aceA gene is knocked out
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
0.002
-
knock-out mutant enzyme after growth on gluconate, crude extract
Pseudomonas putida
4.1.3.1
0.003
-
knock-out mutant enzyme after growth on gluconate + heptanoate, crude extract
Pseudomonas putida
4.1.3.1
0.043
-
wild-type enzyme after growth on gluconate, crude extract
Pseudomonas putida
4.1.3.1
0.321
-
wild-type enzyme after growth on gluconate + heptanoate, crude extract
Pseudomonas putida
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
649361
Pseudomonas putida
succinate + glyoxylate
-
649361
Pseudomonas putida
?
4.1.3.1
isocitrate
rate-limiting enzyme of the citric acid cycle
649361
Pseudomonas putida
succinate + glyoxylate
-
649361
Pseudomonas putida
?
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Pseudomonas putida
rate-limiting enzyme of the citric acid cycle
succinate + glyoxylate
-
Pseudomonas putida
?
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
0.002
-
knock-out mutant enzyme after growth on gluconate, crude extract
Pseudomonas putida
4.1.3.1
0.003
-
knock-out mutant enzyme after growth on gluconate + heptanoate, crude extract
Pseudomonas putida
4.1.3.1
0.043
-
wild-type enzyme after growth on gluconate, crude extract
Pseudomonas putida
4.1.3.1
0.321
-
wild-type enzyme after growth on gluconate + heptanoate, crude extract
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
649361
Pseudomonas putida
succinate + glyoxylate
-
649361
Pseudomonas putida
?
4.1.3.1
isocitrate
rate-limiting enzyme of the citric acid cycle
649361
Pseudomonas putida
succinate + glyoxylate
-
649361
Pseudomonas putida
?