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Literature summary extracted from

  • Song, J.K.; Rhee, J.S.
    Simultaneous enhancement of thermostability and catalytic activity of phospholipase A1 by evolutionary molecular engineering (2000), Appl. Environ. Microbiol., 66, 890-894.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.32 expression in Escherichia coli Serratia sp.
3.1.1.32 expression of wild-type and PCR evolved phospholipase A1 variants in Escherichia coli Serratia sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.32 additional information stability and catalytic activity of phospholipase A1 in the presence of an organic solvent is enhanced by error-prone polymerase chanin reaction and DNA shuffling followed by a filter-based visual screening Serratia sp.
3.1.1.32 additional information thermal stability and catalytic activity of phospholipase A1 are improved by evolutionary molecular engineering Serratia sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.32 0.11
-
phosphatidylcholine 37°C, pH 8.0 Serratia sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.32 phosphatidylcholine + H2O Serratia sp.
-
2-acylglycerophosphocholine + a carboxylate
-
?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.32 acetonitril 50%, approx. 90% loss of activity after 10 h Serratia sp.
3.1.1.32 butyl acetate 50%, approx. 80% loss of activity after 10 h Serratia sp.
3.1.1.32 dimethyl sulfoxide 50%, almost complete loss of activity after 10 h, approx. 90 min half-life Serratia sp.
3.1.1.32 hexane 50%, approx. 85% loss of activity after 10 h Serratia sp.
3.1.1.32 N,N-dimethylformamide 50%, almost complete loss of activity after 10 h Serratia sp.
3.1.1.32 octane 50%, approx. 82% loss of activity after 10 h Serratia sp.
3.1.1.32 toluene 50%, approx. 80% loss of activity after 10 h Serratia sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.32 Serratia sp. Q9X9D8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.32 recombinant His-tagged phospholipase A1 Serratia sp.
3.1.1.32 recombinant phospholipase A1 Serratia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.32 1,2-dimyristoyl-sn-glycero-3-phosphocholine + H2O
-
Serratia sp. 2-myristoyl-sn-glycero-3-phosphocholine + myristic acid
-
?
3.1.1.32 phosphatidylcholine + H2O
-
Serratia sp. 2-acylglycerophosphocholine + a carboxylate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.32 phospholipase A1
-
Serratia sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.32 70
-
loss of 50% activity after 20 min Serratia sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.32 0.057 0.65 phosphatidylcholine 37°C, pH 8.0 Serratia sp.