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Literature summary extracted from

  • Savijoki, K.; Palva, A.
    Purification and molecular characterization of a tripeptidase (PepT) from Lactobacillus helveticus (2000), Appl. Environ. Microbiol., 66, 794-800.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.4
-
 Lactobacillus helveticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.4 1,10-phenanthroline 1 mM, complete inhibition Lactobacillus helveticus
3.4.11.4 2-mercaptoethanol 0.1 mM, 42% inhibition Lactobacillus helveticus
3.4.11.4 Cd2+ 0.1 mM, complete inhibition Lactobacillus helveticus
3.4.11.4 Co2+ 0.1 mM, 72% inhibition Lactobacillus helveticus
3.4.11.4 Cu2+ 0.1 mM, complete inhibition Lactobacillus helveticus
3.4.11.4 dithiothreitol 0.1 mM, 72% inhibition Lactobacillus helveticus
3.4.11.4 EDTA 0.1 mM, complete inhibition Lactobacillus helveticus
3.4.11.4 EGTA 0.1 mM, complete inhibition Lactobacillus helveticus
3.4.11.4 Mn2+ 0.1 mM, 53% inhibition Lactobacillus helveticus
3.4.11.4 phenylmethylsulfonyl fluoride 0.1 mM, 89% inhibition Lactobacillus helveticus
3.4.11.4 Zn2+ 1.0 mM, 91% inhibition Lactobacillus helveticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.4 0.6
-
 Leu-Gly-Gly
-
 Lactobacillus helveticus
3.4.11.4 2.6
-
 Met-Gly-Gly
-
 Lactobacillus helveticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.4 Mg2+ activation to 120% of control be 0.1 mM, activation to 168% of control by 1.0 mM Lactobacillus helveticus
3.4.11.4 Zn2+ activation to 112% of control by 0.1 mM Lactobacillus helveticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.4 47000
-
 3 * 47000, SDS-PAGE Lactobacillus helveticus
3.4.11.4 150000
-
 gel filtration Lactobacillus helveticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.4 Lactobacillus helveticus Q9L4G1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.4
-
 Lactobacillus helveticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.4 additional information
-
 0.0567 per mg per min Lactobacillus helveticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.4 Ala-Ala-Ala + H2O 26% of the activity with Met-Gly-Gly Lactobacillus helveticus Ala + Ala-Ala
-
 ?
3.4.11.4 Gly-Leu-Tyr + H2O 15% of the activity with Met-Gly-Gly Lactobacillus helveticus Gly + Leu-Tyr
-
 ?
3.4.11.4 Leu-Gly-Gly + H2O 34% of the activity with Met-Gly-Gly Lactobacillus helveticus Leu + Gly-Gly
-
 ?
3.4.11.4 Leu-Leu-Ala + H2O 33% of the activity with Met-Gly-Gly Lactobacillus helveticus Leu + Leu-Ala
-
 ?
3.4.11.4 Leu-Leu-Leu + H2O 15% of the activity with Met-Gly-Gly Lactobacillus helveticus Leu + Leu-Leu
-
 ?
3.4.11.4 Lys-Gly-Gly + H2O 13% of the activity with Met-Gly-Gly Lactobacillus helveticus Lys + Gly-Gly
-
 ?
3.4.11.4 Met-Gly-Gly + H2O
-
 Lactobacillus helveticus Met + Gly-Gly
-
 ?
3.4.11.4 Tyr-Gly-Gly + H2O 11% of the activity with Met-Gly-Gly Lactobacillus helveticus Tyr + Gly-Gly
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.4 trimer 3 * 47000, SDS-PAGE Lactobacillus helveticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.4 45
-
 15 min, 55% loss of activity Lactobacillus helveticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.4 7.5
-
-
 Lactobacillus helveticus