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Literature summary extracted from

  • Tkalec, A.L.; Fink, D.; Blain, F.; Zhang-Sun, G.; Laliberte, M.; Bennett, D.C.; Gu, K.; Zimmermann, J.J.F.; Su, H.
    Isolation and expression in Escherichia coli of cslA and cslB, genes coding for the chondroitin sulfate-degrading enzymes chondroitinase AC and chondroitinase B, respectively, from Flavobacterium heparinum (2000), Appl. Environ. Microbiol., 66, 29-35.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.2.5 chondroitin 4-sulfate 2.5fold induction of enzyme expression Pedobacter heparinus
4.2.2.19 chondroitin sulfate A induces enzyme expression in cells Pedobacter heparinus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.5 gene cslA, DNA sequence determination and analysis, chromosomal localization, expression in Escherichia coli Pedobacter heparinus
4.2.2.19 gene cslB, expression in Escherichia coli without the N-terminal signal sequence, different expression plasmids constructed, expression in soluble and insoluble form Pedobacter heparinus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.19 glucose represses enzyme induction in cells by chondroitin sulfate A Pedobacter heparinus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.5 periplasm
-
 Pedobacter heparinus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.5 70000
-
 x * 70000, recombinant enzyme, SDS-PAGE Pedobacter heparinus
4.2.2.19 55000
-
 x * 55000, SDS-PAGE, recombinant and native enzyme Pedobacter heparinus

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.5 Pedobacter heparinus Q59288 gene cslA
-
4.2.2.19 Pedobacter heparinus Q46079 i.e. Cytophaga heparina, Sphingobacterium heparinum, or Pedobacter heparinus, gene cslB
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.2.5 [1,3-beta-D-glucuronic acid-1,4-beta-D-hexosylamine]n = n-1 4-deoxy-beta-D-gluc-4-enuronosyl-1,4-beta-D-hexosylamine + [1,3-beta-D-glucuronic acid-1,4-beta-D-hexosylamine] acts on chondroitin 4-sulfate and chondroitin 6-sulfate, but less well on hyaluronate Pedobacter heparinus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.5 additional information
-
-
 Pedobacter heparinus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.5 chondroitin sulfate A i.e. chondroitin 4-sulfate Pedobacter heparinus ?
-
 ?
4.2.2.5 chondroitin sulfate C i.e. chondroitin 6-sulfate Pedobacter heparinus ?
-
 ?
4.2.2.19 dermatan sulfate
-
 Pedobacter heparinus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.5 ? x * 70000, recombinant enzyme, SDS-PAGE Pedobacter heparinus
4.2.2.19 ? x * 55000, SDS-PAGE, recombinant and native enzyme Pedobacter heparinus

Synonyms

EC Number Synonyms Comment Organism
4.2.2.5 ChnAC
-
 Pedobacter heparinus
4.2.2.5 chondroitinase AC
-
 Pedobacter heparinus
4.2.2.19 ChnB
-
 Pedobacter heparinus
4.2.2.19 chondroitinase B
-
 Pedobacter heparinus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.19 37
-
 recombinant and native enzyme are completely stable Pedobacter heparinus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.5 8
-
 assay at Pedobacter heparinus