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Literature summary extracted from

  • Vollmer, M.D.; Hoier, H.; Hecht, H.J.; Schell, U.; Groning, J.; Goldman, A.; Schlomann, M.
    Substrate specificity of and product formation by muconate cycloisomerases: an analysis of wild-type enzymes and engineered variants (1998), Appl. Environ. Microbiol., 64, 3290-3299.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
5.5.1.1 A271S ratio of turnover number to Km-value: 1.5fold decrease with cis,cis-muconate as substrate, 3.3fold decrease with 2-chloro-cis,cis-muconate as substrate, 27fold increase with 3-chloro-cis,cis-muconate as substrate, 1.9fold decrease with 2,4-dichloro-cis,cis-muconate as substrate, 1.5fold decrease with 3-fluoro-cis,cis-muconate as substrate, 1.1fold increase with 2-methyl-cis,cis-muconate as substrate, 1.1fold decrease with 3-methyl-cis,cis-muconate as substrate Pseudomonas putida
5.5.1.1 F329I ratio of turnover number to Km-value: 5.7fold decrease with cis,cis-muconate as substrate, 1.6fold increase with 2-chloro-cis,cis-muconate as substrate, 3.4fold increase with 3-chloro-cis,cis-muconate as substrate, 3.2fold increase with 2,4-dichloro-cis,cis-muconate as substrate, 1.7fold decrease with 3-fluoro-cis,cis-muconate as substrate, 1.5fold increase with 2-methyl-cis,cis-muconate as substrate, 5.8fold decrease with 3-methyl-cis,cis-muconate as substrate Pseudomonas putida
5.5.1.1 I54V ratio of turnover number to Km-value: 11.9fold decrease with cis,cis-muconate as substrate, nearly identical to wild-type value with 2-chloro-cis,cis-muconate and 3-methyl-cis,cis-muconate as substrate, 22fold increase with 3-chloro-cis,cis-muconate as substrate, 1.67fold decrease with 2,4-dichloro-cis,cis-muconate as substrate, 5.8fold decrease with 3-fluoro-cis,cis-muconate as substrate, 8.8fold decrease with 2-methyl-cis,cis-muconate as substrate Pseudomonas putida
5.5.1.1 I54V/F329I ratio of turnover number to Km-value: 81.5fold decrease with cis,cis-muconate as substrate, 2.6fold decrease with 2-chloro-cis,cis-muconate as substrate, 2.7fold increase with 3-chloro-cis,cis-muconate as substrate, 3.8fold increase with 2,4-dichloro-cis,cis-muconate as substrate, 2fold decrease with 3-fluoro-cis,cis-muconate as substrate, 4fold decrease with 2-methyl-cis,cis-muconate as substrate, 2fold decrease with 3-methyl-cis,cis-muconate as substrate Pseudomonas putida
5.5.1.1 K276N ratio of turnover number to Km-value: 10.7fold decrease with cis,cis-muconate as substrate, 8.7fold decrease with 2-chloro-cis,cis-muconate as substrate, 3.5fold increase with 3-chloro-cis,cis-muconate as substrate, 8.7fold decrease with 2,4-dichloro-cis,cis-muconate as substrate, 4.1fold decrease with 3-fluoro-cis,cis-muconate as substrate, 16.7fold decrease with 2-methyl-cis,cis-muconate as substrate, 12.1fold decrease with 3-methyl-cis,cis-muconate as substrate Pseudomonas putida
5.5.1.1 L333V ratio of turnover number to Km-value: 238fold decrease with cis,cis-muconate as substrate, 41.5fold decrease with 2-chloro-cis,cis-muconate as substrate, 3.4fold increase with 3-chloro-cis,cis-muconate as substrate, 5.9fold decrease with 2,4-dichloro-cis,cis-muconate as substrate, 1.4fold decrease with 3-fluoro-cis,cis-muconate as substrate, 34.6fold decrease with 2-methyl-cis,cis-muconate as substrate, 16.5fold decrease with 3-methyl-cis,cis-muconate as substrate Pseudomonas putida
5.5.1.1 Y59W ratio of turnover number to Km-value: 12.9fold decrease with cis,cis-muconate as substrate, nearly identical to wild-type value with 2-chloro-cis,cis-muconate as substrate, 4.4fold decrease with 3-fluoro-cis,cis-muconate, 1.8fold decrease with 2-methyl-cis,cis-muconate as substrate, 10.2fold decrease with 3-methyl-cis,cis-muconate as substrate Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.5.1.1 2,4-dichloro-cis,cis-muconate inhibitor of cis,cis-muconate conversion Acinetobacter calcoaceticus
5.5.1.1 2,4-dichloro-cis,cis-muconate inhibitor of cis,cis-muconate conversion Pseudomonas putida
5.5.1.1 2-fluoro-cis,cis-muconate inhibitor of cis,cis-muconate conversion Acinetobacter calcoaceticus
5.5.1.1 2-fluoro-cis,cis-muconate inhibitor of cis,cis-muconate conversion Pseudomonas putida
5.5.1.1 3-chloro-cis,cis-muconate inhibitor of cis,cis-muconate conversion Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5.1.1 0.006
-
 3-methyl-cis,cis-muconate pH 7.5, 25°C, wild-type enzyme Pseudomonas putida
5.5.1.1 0.0064
-
 3-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme A271S or I54V/F329 Pseudomonas putida
5.5.1.1 0.0072
-
 3-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme I54V Pseudomonas putida
5.5.1.1 0.0091
-
 3-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme L333V Pseudomonas putida
5.5.1.1 0.0097
-
 3-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme A271S Pseudomonas putida
5.5.1.1 0.012
-
 3-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme K276N Pseudomonas putida
5.5.1.1 0.015
-
 3-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme Y59W Pseudomonas putida
5.5.1.1 0.016
-
 3-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme F329I Pseudomonas putida
5.5.1.1 0.017
-
 2-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme Y59W Pseudomonas putida
5.5.1.1 0.026
-
 2,4-dichloro-cis,cis-muconate pH 7.5, 25°C, wild-type enzyme Pseudomonas putida
5.5.1.1 0.033
-
 2-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme I54V/F329I Pseudomonas putida
5.5.1.1 0.034
-
 2-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme K276N Pseudomonas putida
5.5.1.1 0.035
-
 cis,cis-muconate pH 7.5, 25°C, mutant enzyme A271S Pseudomonas putida
5.5.1.1 0.036
-
 2-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme L333V Pseudomonas putida
5.5.1.1 0.037
-
 2-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme F329I Pseudomonas putida
5.5.1.1 0.039
-
 2,4-dichloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme L333V Pseudomonas putida
5.5.1.1 0.04
-
 2,4-dichloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme K276N Pseudomonas putida
5.5.1.1 0.04
-
 cis,cis-muconate pH 7.5, 25°C, wild-type enzyme Pseudomonas putida
5.5.1.1 0.04
-
 2-methyl-cis,cis-muconate pH 7.5, 25°C, wild-type enzyme Pseudomonas putida
5.5.1.1 0.044
-
 2,4-dichloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme A271S Pseudomonas putida
5.5.1.1 0.045
-
 3-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme I54V Pseudomonas putida
5.5.1.1 0.045
-
 2,4-dichloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme I54V/F329I Pseudomonas putida
5.5.1.1 0.047
-
 2-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme I54V Pseudomonas putida
5.5.1.1 0.047
-
 cis,cis-muconate pH 7.5, 25°C, mutant enzyme Y59W and F329I Pseudomonas putida
5.5.1.1 0.052
-
 cis,cis-muconate pH 7.5, 25°C, mutant enzyme L333V Pseudomonas putida
5.5.1.1 0.057
-
 2-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme A271S or I54V/F329 Pseudomonas putida
5.5.1.1 0.058
-
 2-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme I54V Pseudomonas putida
5.5.1.1 0.059
-
 2-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme A271S Pseudomonas putida
5.5.1.1 0.059
-
 2-chloro-cis,cis-muconate pH 7.5, 25°C, wild-type enzyme Pseudomonas putida
5.5.1.1 0.06
-
 2-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme A271S Pseudomonas putida
5.5.1.1 0.063
-
 3-fluoro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme L333V Pseudomonas putida
5.5.1.1 0.068
-
 2-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme F329I Pseudomonas putida
5.5.1.1 0.069
-
 2-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme Y59W Pseudomonas putida
5.5.1.1 0.074
-
 2,4-dichloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme I54V Pseudomonas putida
5.5.1.1 0.074
-
 cis,cis-muconate pH 7.5, 25°C, mutant enzyme K276N Pseudomonas putida
5.5.1.1 0.076
-
 3-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme L333V Pseudomonas putida
5.5.1.1 0.078
-
 2,4-dichloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme F329I Pseudomonas putida
5.5.1.1 0.093
-
 2,4-dichloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme I54V Pseudomonas putida
5.5.1.1 0.096
-
 cis,cis-muconate pH 7.5, 25°C, mutant enzyme I54V Pseudomonas putida
5.5.1.1 0.097
-
 3-fluoro-cis,cis-muconate pH 7.5, 25°C, wild-type enzyme Pseudomonas putida
5.5.1.1 0.099
-
 3-methyl-cis,cis-muconate pH 7.5, 25°C Acinetobacter calcoaceticus
5.5.1.1 0.1
-
 3-fluoro-cis,cis-muconate pH 7.5, 25°C Acinetobacter calcoaceticus
5.5.1.1 0.1
-
 3-fluoro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme A271S or I54V/F329 Pseudomonas putida
5.5.1.1 0.11
-
 2-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme L333V Pseudomonas putida
5.5.1.1 0.13
-
 cis,cis-muconate pH 7.5, 25°C Acinetobacter calcoaceticus
5.5.1.1 0.14
-
 cis,cis-muconate pH 7.5, 25°C, mutant enzyme I54V/F329I Pseudomonas putida
5.5.1.1 0.15
-
 3-fluoro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme F329I Pseudomonas putida
5.5.1.1 0.15
-
 3-fluoro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme Y59W Pseudomonas putida
5.5.1.1 0.18
-
 3-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme A271S Pseudomonas putida
5.5.1.1 0.18
-
 2-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme K276N Pseudomonas putida
5.5.1.1 0.21
-
 3-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme I54V/F329I Pseudomonas putida
5.5.1.1 0.23
-
 3-fluoro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme K276N Pseudomonas putida
5.5.1.1 0.29
-
 2-chloro-cis,cis-muconate pH 7.5, 25°C Acinetobacter calcoaceticus
5.5.1.1 0.32
-
 3-chloro-cis,cis-muconate pH 7.5, 25°C, wild-type enzyme Pseudomonas putida
5.5.1.1 0.49
-
 2-methyl-cis,cis-muconate pH 7.5, 25°C Acinetobacter calcoaceticus
5.5.1.1 0.55
-
 3-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme K276N Pseudomonas putida
5.5.1.1 0.69
-
 3-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme F329I Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.5.1.1 cis,cis-muconate Pseudomonas putida the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone muconolactone
-
 ?
5.5.1.1 cis,cis-muconate Acinetobacter calcoaceticus the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone muconolactone
-
 ?
5.5.1.1 cis,cis-muconate Pseudomonas putida PRS2000 the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone muconolactone
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.1 Acinetobacter calcoaceticus
-
-
-
5.5.1.1 Pseudomonas putida
-
 PRS2000
-
5.5.1.1 Pseudomonas putida PRS2000
-
 PRS2000
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.1
-
 Pseudomonas putida
5.5.1.1
-
 Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.1 2,4-dichloro-cis,cis-muconate
-
 Pseudomonas putida ?
-
 ?
5.5.1.1 2,4-dichloro-cis,cis-muconate
-
 Pseudomonas putida PRS2000 ?
-
 ?
5.5.1.1 2-Chloro-cis,cis-muconate
-
 Acinetobacter calcoaceticus ?
-
 ?
5.5.1.1 2-chloro-cis,cis-muconate
-
 Pseudomonas putida (+)-2-chloromuconolactone + (+)-5-chloromuconolactone mutant enzymes I54V and I54V/Y59W form 5-chloromuconolactone almost exclusively. Mutant enzymes F329I and Y59W form 2-chloromuconolactone as the main product. Mutant enzyme A271S hehaves similar to wild-type enzyme ?
5.5.1.1 2-Methyl-cis,cis-muconate
-
 Acinetobacter calcoaceticus ?
-
 ?
5.5.1.1 2-methyl-cis,cis-muconate
-
 Pseudomonas putida 2-methylmuconolactone
-
 ?
5.5.1.1 3-chloro-cis,cis-muconate
-
 Pseudomonas putida protoanemonin + ? formed by wild-type enzyme and mutant enzymes I54V, A271S, K276N, F329I, L333V, I54V/F329I ?
5.5.1.1 3-Fluoro-cis,cis-muconate
-
 Pseudomonas putida ?
-
 ?
5.5.1.1 3-Fluoro-cis,cis-muconate
-
 Acinetobacter calcoaceticus ?
-
 ?
5.5.1.1 3-Fluoro-cis,cis-muconate
-
 Pseudomonas putida PRS2000 ?
-
 ?
5.5.1.1 3-Methyl-cis,cis-muconate
-
 Pseudomonas putida ?
-
 ?
5.5.1.1 3-Methyl-cis,cis-muconate
-
 Acinetobacter calcoaceticus ?
-
 ?
5.5.1.1 3-Methyl-cis,cis-muconate
-
 Pseudomonas putida PRS2000 ?
-
 ?
5.5.1.1 cis,cis-muconate
-
 Pseudomonas putida muconolactone
-
 ?
5.5.1.1 cis,cis-muconate
-
 Acinetobacter calcoaceticus muconolactone
-
 ?
5.5.1.1 cis,cis-muconate the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone Pseudomonas putida muconolactone
-
 ?
5.5.1.1 cis,cis-muconate the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone Acinetobacter calcoaceticus muconolactone
-
 ?
5.5.1.1 cis,cis-muconate
-
 Pseudomonas putida PRS2000 muconolactone
-
 ?
5.5.1.1 cis,cis-muconate the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone Pseudomonas putida PRS2000 muconolactone
-
 ?
5.5.1.1 additional information no activity with 3-chloro-cis,cis-muconate and 2,4-dichloro-cis,cis-muconate Acinetobacter calcoaceticus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.5.1.1 ADP1
-
 Acinetobacter calcoaceticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5.1.1 0.4
-
 2,4-dichloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme L333V Pseudomonas putida
5.5.1.1 3.4
-
 2,4-dichloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme K276N Pseudomonas putida
5.5.1.1 36.6
-
 2,4-dichloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme K276N Pseudomonas putida
5.5.1.1 43.8
-
 2-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme L333V Pseudomonas putida
5.5.1.1 52.8
-
 2,4-dichloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme L333V Pseudomonas putida
5.5.1.1 174
-
 2,4-dichloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme A271S Pseudomonas putida
5.5.1.1 192
-
 2-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme K276N Pseudomonas putida
5.5.1.1 198
-
 2,4-dichloro-cis,cis-muconate pH 7.5, 25°C, wild-type enzyme Pseudomonas putida
5.5.1.1 432
-
 2,4-dichloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme I54V Pseudomonas putida
5.5.1.1 600
-
 2-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme I54V/F329I Pseudomonas putida
5.5.1.1 900
-
 2-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme Y59W and A271S Pseudomonas putida
5.5.1.1 1020
-
 2-chloro-cis,cis-muconate pH 7.5, 25°C Acinetobacter calcoaceticus
5.5.1.1 1320
-
 2,4-dichloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme I54V/F329I Pseudomonas putida
5.5.1.1 1920
-
 2,4-dichloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme F329I Pseudomonas putida
5.5.1.1 2100
-
 2-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme I54V Pseudomonas putida
5.5.1.1 2880
-
 2-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme F329I Pseudomonas putida
5.5.1.1 2940
-
 2-chloro-cis,cis-muconate pH 7.5, 25°C, wild-type enzyme Pseudomonas putida
5.5.1.1 3120
-
 3-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme L333V Pseudomonas putida
5.5.1.1 3240
-
 3-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme L333V Pseudomonas putida
5.5.1.1 3840
-
 3-chloro-cis,cis-muconate pH 7.5, 25°C, wild-type enzyme Pseudomonas putida
5.5.1.1 4080
-
 cis,cis-muconate pH 7.5, 25°C, mutant enzyme L333V Pseudomonas putida
5.5.1.1 5700
-
 3-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme K276N Pseudomonas putida
5.5.1.1 6600
-
 3-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme I54V/F329I Pseudomonas putida
5.5.1.1 8400
-
 3-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme Y59W Pseudomonas putida
5.5.1.1 12000
-
 3-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme I54V Pseudomonas putida
5.5.1.1 16200
-
 3-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme F329I Pseudomonas putida
5.5.1.1 18600
-
 3-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme A271S or I54V/F329 Pseudomonas putida
5.5.1.1 19200
-
 2-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme L333V Pseudomonas putida
5.5.1.1 22800
-
 3-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme K276N Pseudomonas putida
5.5.1.1 27600
-
 3-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme F329I Pseudomonas putida
5.5.1.1 32400
-
 cis,cis-muconate pH 7.5, 25°C, mutant enzyme I54V/F329I Pseudomonas putida
5.5.1.1 35400
-
 3-methyl-cis,cis-muconate pH 7.5, 25°C, wild-type enzyme Pseudomonas putida
5.5.1.1 39000
-
 2-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme I54V Pseudomonas putida
5.5.1.1 40800
-
 3-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme A271S Pseudomonas putida
5.5.1.1 44400
-
 3-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme I54V Pseudomonas putida
5.5.1.1 57600
-
 3-chloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme A271S Pseudomonas putida
5.5.1.1 66000
-
 cis,cis-muconate pH 7.5, 25°C, mutant enzyme Y59W Pseudomonas putida
5.5.1.1 72000
-
 2,4-dichloro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme I54V Pseudomonas putida
5.5.1.1 84000
-
 2-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme A271S or I54V/F329 Pseudomonas putida
5.5.1.1 108000
-
 3-methyl-cis,cis-muconate pH 7.5, 25°C Acinetobacter calcoaceticus
5.5.1.1 126000
-
 cis,cis-muconate pH 7.5, 25°C, mutant enzyme K276N Pseudomonas putida
5.5.1.1 150000
-
 cis,cis-muconate pH 7.5, 25°C, mutant enzyme I54V Pseudomonas putida
5.5.1.1 156000
-
 3-fluoro-cis,cis-muconate pH 7.5, 25°C Acinetobacter calcoaceticus
5.5.1.1 156000
-
 cis,cis-muconate pH 7.5, 25°C, mutant enzyme F329I Pseudomonas putida
5.5.1.1 192000
-
 3-fluoro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme Y59W Pseudomonas putida
5.5.1.1 202000
-
 2-methyl-cis,cis-muconate pH 7.5, 25°C Acinetobacter calcoaceticus
5.5.1.1 222000
-
 cis,cis-muconate pH 7.5, 25°C Acinetobacter calcoaceticus
5.5.1.1 222000
-
 2-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme Y59W Pseudomonas putida
5.5.1.1 234000
-
 2-methyl-cis,cis-muconate pH 7.5, 25°C, wild-type enzyme Pseudomonas putida
5.5.1.1 246000
-
 3-fluoro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme L333V Pseudomonas putida
5.5.1.1 294000
-
 3-fluoro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme I54V/F329I Pseudomonas putida
5.5.1.1 304000
-
 2-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme A271S Pseudomonas putida
5.5.1.1 312000
-
 3-fluoro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme K276N Pseudomonas putida
5.5.1.1 360000
-
 3-fluoro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme A271S Pseudomonas putida
5.5.1.1 426000
-
 cis,cis-muconate pH 7.5, 25°C, mutant enzyme A271S Pseudomonas putida
5.5.1.1 480000
-
 3-fluoro-cis,cis-muconate pH 7.5, 25°C, mutant enzyme F329I Pseudomonas putida
5.5.1.1 534000
-
 3-fluoro-cis,cis-muconate pH 7.5, 25°C, wild-type enzyme Pseudomonas putida
5.5.1.1 600000
-
 2-methyl-cis,cis-muconate pH 7.5, 25°C, mutant enzyme K276N or F329I Pseudomonas putida
5.5.1.1 756000
-
 cis,cis-muconate pH 7.5, 25°C, wild-type enzyme Pseudomonas putida

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.5.1.1 0.0035
-
 2,4-dichloro-cis,cis-muconate inhibitor of cis,cis-muconate conversion Pseudomonas putida
5.5.1.1 0.027
-
 2-fluoro-cis,cis-muconate inhibitor of cis,cis-muconate conversion Acinetobacter calcoaceticus
5.5.1.1 0.031
-
 2-fluoro-cis,cis-muconate inhibitor of cis,cis-muconate conversion Pseudomonas putida
5.5.1.1 0.035
-
 2,4-dichloro-cis,cis-muconate inhibitor of cis,cis-muconate conversion Acinetobacter calcoaceticus
5.5.1.1 0.095
-
 3-chloro-cis,cis-muconate inhibitor of cis,cis-muconate conversion Pseudomonas putida