Substrate specificity of and product formation by muconate cycloisomerases: an analysis of wild-type enzymes and engineered variants
Vollmer, M.D.; Hoier, H.; Hecht, H.J.; Schell, U.; Groning, J.; Goldman, A.; Schlomann, M.; Appl. Environ. Microbiol. 64, 3290-3299 (1998) 
Data extracted from this reference:
Engineering
EC Number
Protein Variants
Commentary
Organism
5.5.1.1
A271S
ratio of turnover number to Km-value: 1.5fold decrease with cis,cis-muconate as substrate, 3.3fold decrease with 2-chloro-cis,cis-muconate as substrate, 27fold increase with 3-chloro-cis,cis-muconate as substrate, 1.9fold decrease with 2,4-dichloro-cis,cis-muconate as substrate, 1.5fold decrease with 3-fluoro-cis,cis-muconate as substrate, 1.1fold increase with 2-methyl-cis,cis-muconate as substrate, 1.1fold decrease with 3-methyl-cis,cis-muconate as substrate
Pseudomonas putida
5.5.1.1
F329I
ratio of turnover number to Km-value: 5.7fold decrease with cis,cis-muconate as substrate, 1.6fold increase with 2-chloro-cis,cis-muconate as substrate, 3.4fold increase with 3-chloro-cis,cis-muconate as substrate, 3.2fold increase with 2,4-dichloro-cis,cis-muconate as substrate, 1.7fold decrease with 3-fluoro-cis,cis-muconate as substrate, 1.5fold increase with 2-methyl-cis,cis-muconate as substrate, 5.8fold decrease with 3-methyl-cis,cis-muconate as substrate
Pseudomonas putida
5.5.1.1
I54V
ratio of turnover number to Km-value: 11.9fold decrease with cis,cis-muconate as substrate, nearly identical to wild-type value with 2-chloro-cis,cis-muconate and 3-methyl-cis,cis-muconate as substrate, 22fold increase with 3-chloro-cis,cis-muconate as substrate, 1.67fold decrease with 2,4-dichloro-cis,cis-muconate as substrate, 5.8fold decrease with 3-fluoro-cis,cis-muconate as substrate, 8.8fold decrease with 2-methyl-cis,cis-muconate as substrate
Pseudomonas putida
5.5.1.1
I54V/F329I
ratio of turnover number to Km-value: 81.5fold decrease with cis,cis-muconate as substrate, 2.6fold decrease with 2-chloro-cis,cis-muconate as substrate, 2.7fold increase with 3-chloro-cis,cis-muconate as substrate, 3.8fold increase with 2,4-dichloro-cis,cis-muconate as substrate, 2fold decrease with 3-fluoro-cis,cis-muconate as substrate, 4fold decrease with 2-methyl-cis,cis-muconate as substrate, 2fold decrease with 3-methyl-cis,cis-muconate as substrate
Pseudomonas putida
5.5.1.1
K276N
ratio of turnover number to Km-value: 10.7fold decrease with cis,cis-muconate as substrate, 8.7fold decrease with 2-chloro-cis,cis-muconate as substrate, 3.5fold increase with 3-chloro-cis,cis-muconate as substrate, 8.7fold decrease with 2,4-dichloro-cis,cis-muconate as substrate, 4.1fold decrease with 3-fluoro-cis,cis-muconate as substrate, 16.7fold decrease with 2-methyl-cis,cis-muconate as substrate, 12.1fold decrease with 3-methyl-cis,cis-muconate as substrate
Pseudomonas putida
5.5.1.1
L333V
ratio of turnover number to Km-value: 238fold decrease with cis,cis-muconate as substrate, 41.5fold decrease with 2-chloro-cis,cis-muconate as substrate, 3.4fold increase with 3-chloro-cis,cis-muconate as substrate, 5.9fold decrease with 2,4-dichloro-cis,cis-muconate as substrate, 1.4fold decrease with 3-fluoro-cis,cis-muconate as substrate, 34.6fold decrease with 2-methyl-cis,cis-muconate as substrate, 16.5fold decrease with 3-methyl-cis,cis-muconate as substrate
Pseudomonas putida
5.5.1.1
Y59W
ratio of turnover number to Km-value: 12.9fold decrease with cis,cis-muconate as substrate, nearly identical to wild-type value with 2-chloro-cis,cis-muconate as substrate, 4.4fold decrease with 3-fluoro-cis,cis-muconate, 1.8fold decrease with 2-methyl-cis,cis-muconate as substrate, 10.2fold decrease with 3-methyl-cis,cis-muconate as substrate
Pseudomonas putida
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.5.1.1
2,4-dichloro-cis,cis-muconate
inhibitor of cis,cis-muconate conversion
Acinetobacter calcoaceticus
5.5.1.1
2,4-dichloro-cis,cis-muconate
inhibitor of cis,cis-muconate conversion
Pseudomonas putida
5.5.1.1
2-fluoro-cis,cis-muconate
inhibitor of cis,cis-muconate conversion
Acinetobacter calcoaceticus
5.5.1.1
2-fluoro-cis,cis-muconate
inhibitor of cis,cis-muconate conversion
Pseudomonas putida
5.5.1.1
3-chloro-cis,cis-muconate
inhibitor of cis,cis-muconate conversion
Pseudomonas putida
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.5.1.1
0.006
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
0.0064
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S or I54V/F329
Pseudomonas putida
5.5.1.1
0.0072
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
0.0091
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
0.0097
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S
Pseudomonas putida
5.5.1.1
0.012
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
0.015
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme Y59W
Pseudomonas putida
5.5.1.1
0.016
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme F329I
Pseudomonas putida
5.5.1.1
0.017
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme Y59W
Pseudomonas putida
5.5.1.1
0.026
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
0.033
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V/F329I
Pseudomonas putida
5.5.1.1
0.034
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
0.035
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S
Pseudomonas putida
5.5.1.1
0.036
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
0.037
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme F329I
Pseudomonas putida
5.5.1.1
0.039
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
0.04
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
0.04
-
cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
0.04
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
0.044
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S
Pseudomonas putida
5.5.1.1
0.045
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
0.045
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V/F329I
Pseudomonas putida
5.5.1.1
0.047
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
0.047
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme Y59W and F329I
Pseudomonas putida
5.5.1.1
0.052
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
0.057
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S or I54V/F329
Pseudomonas putida
5.5.1.1
0.058
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
0.059
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S
Pseudomonas putida
5.5.1.1
0.059
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
0.06
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S
Pseudomonas putida
5.5.1.1
0.063
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
0.068
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme F329I
Pseudomonas putida
5.5.1.1
0.069
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme Y59W
Pseudomonas putida
5.5.1.1
0.074
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
0.074
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
0.076
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
0.078
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme F329I
Pseudomonas putida
5.5.1.1
0.093
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
0.096
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
0.097
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
0.099
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C
Acinetobacter calcoaceticus
5.5.1.1
0.1
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C
Acinetobacter calcoaceticus
5.5.1.1
0.1
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S or I54V/F329
Pseudomonas putida
5.5.1.1
0.11
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
0.13
-
cis,cis-muconate
pH 7.5, 25°C
Acinetobacter calcoaceticus
5.5.1.1
0.14
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V/F329I
Pseudomonas putida
5.5.1.1
0.15
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme F329I
Pseudomonas putida
5.5.1.1
0.15
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme Y59W
Pseudomonas putida
5.5.1.1
0.18
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S
Pseudomonas putida
5.5.1.1
0.18
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
0.21
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V/F329I
Pseudomonas putida
5.5.1.1
0.23
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
0.29
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C
Acinetobacter calcoaceticus
5.5.1.1
0.32
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
0.49
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C
Acinetobacter calcoaceticus
5.5.1.1
0.55
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
0.69
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme F329I
Pseudomonas putida
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5.5.1.1
cis,cis-muconate
Pseudomonas putida
the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone
muconolactone
-
Pseudomonas putida
?
5.5.1.1
cis,cis-muconate
Acinetobacter calcoaceticus
the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone
muconolactone
-
Acinetobacter calcoaceticus
?
5.5.1.1
cis,cis-muconate
Pseudomonas putida PRS2000
the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone
muconolactone
-
Pseudomonas putida PRS2000
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
5.5.1.1
Acinetobacter calcoaceticus
-
-
-
5.5.1.1
Pseudomonas putida
-
PRS2000
-
5.5.1.1
Pseudomonas putida PRS2000
-
PRS2000
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
5.5.1.1
-
Pseudomonas putida
5.5.1.1
-
Acinetobacter calcoaceticus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5.5.1.1
2,4-dichloro-cis,cis-muconate
-
649348
Pseudomonas putida
?
-
649348
Pseudomonas putida
?
5.5.1.1
2,4-dichloro-cis,cis-muconate
-
649348
Pseudomonas putida PRS2000
?
-
649348
Pseudomonas putida PRS2000
?
5.5.1.1
2-Chloro-cis,cis-muconate
-
649348
Acinetobacter calcoaceticus
?
-
649348
Acinetobacter calcoaceticus
?
5.5.1.1
2-chloro-cis,cis-muconate
-
649348
Pseudomonas putida
(+)-2-chloromuconolactone + (+)-5-chloromuconolactone
mutant enzymes I54V and I54V/Y59W form 5-chloromuconolactone almost exclusively. Mutant enzymes F329I and Y59W form 2-chloromuconolactone as the main product. Mutant enzyme A271S hehaves similar to wild-type enzyme
649348
Pseudomonas putida
?
5.5.1.1
2-Methyl-cis,cis-muconate
-
649348
Acinetobacter calcoaceticus
?
-
649348
Acinetobacter calcoaceticus
?
5.5.1.1
2-methyl-cis,cis-muconate
-
649348
Pseudomonas putida
2-methylmuconolactone
-
649348
Pseudomonas putida
?
5.5.1.1
3-chloro-cis,cis-muconate
-
649348
Pseudomonas putida
protoanemonin + ?
formed by wild-type enzyme and mutant enzymes I54V, A271S, K276N, F329I, L333V, I54V/F329I
649348
Pseudomonas putida
?
5.5.1.1
3-Fluoro-cis,cis-muconate
-
649348
Pseudomonas putida
?
-
649348
Pseudomonas putida
?
5.5.1.1
3-Fluoro-cis,cis-muconate
-
649348
Acinetobacter calcoaceticus
?
-
649348
Acinetobacter calcoaceticus
?
5.5.1.1
3-Fluoro-cis,cis-muconate
-
649348
Pseudomonas putida PRS2000
?
-
649348
Pseudomonas putida PRS2000
?
5.5.1.1
3-Methyl-cis,cis-muconate
-
649348
Pseudomonas putida
?
-
649348
Pseudomonas putida
?
5.5.1.1
3-Methyl-cis,cis-muconate
-
649348
Acinetobacter calcoaceticus
?
-
649348
Acinetobacter calcoaceticus
?
5.5.1.1
3-Methyl-cis,cis-muconate
-
649348
Pseudomonas putida PRS2000
?
-
649348
Pseudomonas putida PRS2000
?
5.5.1.1
cis,cis-muconate
-
649348
Pseudomonas putida
muconolactone
-
649348
Pseudomonas putida
?
5.5.1.1
cis,cis-muconate
-
649348
Acinetobacter calcoaceticus
muconolactone
-
649348
Acinetobacter calcoaceticus
?
5.5.1.1
cis,cis-muconate
the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone
649348
Pseudomonas putida
muconolactone
-
649348
Pseudomonas putida
?
5.5.1.1
cis,cis-muconate
the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone
649348
Acinetobacter calcoaceticus
muconolactone
-
649348
Acinetobacter calcoaceticus
?
5.5.1.1
cis,cis-muconate
-
649348
Pseudomonas putida PRS2000
muconolactone
-
649348
Pseudomonas putida PRS2000
?
5.5.1.1
cis,cis-muconate
the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone
649348
Pseudomonas putida PRS2000
muconolactone
-
649348
Pseudomonas putida PRS2000
?
5.5.1.1
additional information
no activity with 3-chloro-cis,cis-muconate and 2,4-dichloro-cis,cis-muconate
649348
Acinetobacter calcoaceticus
?
-
649348
Acinetobacter calcoaceticus
?
Synonyms
EC Number
Synonyms
Commentary
Organism
5.5.1.1
ADP1
-
Acinetobacter calcoaceticus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.5.1.1
0.4
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
3.4
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
36.6
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
43.8
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
52.8
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
174
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S
Pseudomonas putida
5.5.1.1
192
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
198
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
432
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
600
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V/F329I
Pseudomonas putida
5.5.1.1
900
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme Y59W and A271S
Pseudomonas putida
5.5.1.1
1020
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C
Acinetobacter calcoaceticus
5.5.1.1
1320
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V/F329I
Pseudomonas putida
5.5.1.1
1920
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme F329I
Pseudomonas putida
5.5.1.1
2100
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
2880
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme F329I
Pseudomonas putida
5.5.1.1
2940
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
3120
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
3240
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
3840
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
4080
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
5700
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
6600
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V/F329I
Pseudomonas putida
5.5.1.1
8400
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme Y59W
Pseudomonas putida
5.5.1.1
12000
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
16200
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme F329I
Pseudomonas putida
5.5.1.1
18600
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S or I54V/F329
Pseudomonas putida
5.5.1.1
19200
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
22800
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
27600
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme F329I
Pseudomonas putida
5.5.1.1
32400
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V/F329I
Pseudomonas putida
5.5.1.1
35400
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
39000
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
40800
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S
Pseudomonas putida
5.5.1.1
44400
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
57600
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S
Pseudomonas putida
5.5.1.1
66000
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme Y59W
Pseudomonas putida
5.5.1.1
72000
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
84000
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S or I54V/F329
Pseudomonas putida
5.5.1.1
108000
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C
Acinetobacter calcoaceticus
5.5.1.1
126000
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
150000
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
156000
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C
Acinetobacter calcoaceticus
5.5.1.1
156000
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme F329I
Pseudomonas putida
5.5.1.1
192000
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme Y59W
Pseudomonas putida
5.5.1.1
202000
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C
Acinetobacter calcoaceticus
5.5.1.1
222000
-
cis,cis-muconate
pH 7.5, 25°C
Acinetobacter calcoaceticus
5.5.1.1
222000
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme Y59W
Pseudomonas putida
5.5.1.1
234000
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
246000
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
294000
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V/F329I
Pseudomonas putida
5.5.1.1
304000
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S
Pseudomonas putida
5.5.1.1
312000
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
360000
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S
Pseudomonas putida
5.5.1.1
426000
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S
Pseudomonas putida
5.5.1.1
480000
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme F329I
Pseudomonas putida
5.5.1.1
534000
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
600000
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N or F329I
Pseudomonas putida
5.5.1.1
756000
-
cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
5.5.1.1
0.0035
-
2,4-dichloro-cis,cis-muconate
inhibitor of cis,cis-muconate conversion
Pseudomonas putida
5.5.1.1
0.027
-
2-fluoro-cis,cis-muconate
inhibitor of cis,cis-muconate conversion
Acinetobacter calcoaceticus
5.5.1.1
0.031
-
2-fluoro-cis,cis-muconate
inhibitor of cis,cis-muconate conversion
Pseudomonas putida
5.5.1.1
0.035
-
2,4-dichloro-cis,cis-muconate
inhibitor of cis,cis-muconate conversion
Acinetobacter calcoaceticus
5.5.1.1
0.095
-
3-chloro-cis,cis-muconate
inhibitor of cis,cis-muconate conversion
Pseudomonas putida
Engineering (protein specific)
EC Number
Protein Variants
Commentary
Organism
5.5.1.1
A271S
ratio of turnover number to Km-value: 1.5fold decrease with cis,cis-muconate as substrate, 3.3fold decrease with 2-chloro-cis,cis-muconate as substrate, 27fold increase with 3-chloro-cis,cis-muconate as substrate, 1.9fold decrease with 2,4-dichloro-cis,cis-muconate as substrate, 1.5fold decrease with 3-fluoro-cis,cis-muconate as substrate, 1.1fold increase with 2-methyl-cis,cis-muconate as substrate, 1.1fold decrease with 3-methyl-cis,cis-muconate as substrate
Pseudomonas putida
5.5.1.1
F329I
ratio of turnover number to Km-value: 5.7fold decrease with cis,cis-muconate as substrate, 1.6fold increase with 2-chloro-cis,cis-muconate as substrate, 3.4fold increase with 3-chloro-cis,cis-muconate as substrate, 3.2fold increase with 2,4-dichloro-cis,cis-muconate as substrate, 1.7fold decrease with 3-fluoro-cis,cis-muconate as substrate, 1.5fold increase with 2-methyl-cis,cis-muconate as substrate, 5.8fold decrease with 3-methyl-cis,cis-muconate as substrate
Pseudomonas putida
5.5.1.1
I54V
ratio of turnover number to Km-value: 11.9fold decrease with cis,cis-muconate as substrate, nearly identical to wild-type value with 2-chloro-cis,cis-muconate and 3-methyl-cis,cis-muconate as substrate, 22fold increase with 3-chloro-cis,cis-muconate as substrate, 1.67fold decrease with 2,4-dichloro-cis,cis-muconate as substrate, 5.8fold decrease with 3-fluoro-cis,cis-muconate as substrate, 8.8fold decrease with 2-methyl-cis,cis-muconate as substrate
Pseudomonas putida
5.5.1.1
I54V/F329I
ratio of turnover number to Km-value: 81.5fold decrease with cis,cis-muconate as substrate, 2.6fold decrease with 2-chloro-cis,cis-muconate as substrate, 2.7fold increase with 3-chloro-cis,cis-muconate as substrate, 3.8fold increase with 2,4-dichloro-cis,cis-muconate as substrate, 2fold decrease with 3-fluoro-cis,cis-muconate as substrate, 4fold decrease with 2-methyl-cis,cis-muconate as substrate, 2fold decrease with 3-methyl-cis,cis-muconate as substrate
Pseudomonas putida
5.5.1.1
K276N
ratio of turnover number to Km-value: 10.7fold decrease with cis,cis-muconate as substrate, 8.7fold decrease with 2-chloro-cis,cis-muconate as substrate, 3.5fold increase with 3-chloro-cis,cis-muconate as substrate, 8.7fold decrease with 2,4-dichloro-cis,cis-muconate as substrate, 4.1fold decrease with 3-fluoro-cis,cis-muconate as substrate, 16.7fold decrease with 2-methyl-cis,cis-muconate as substrate, 12.1fold decrease with 3-methyl-cis,cis-muconate as substrate
Pseudomonas putida
5.5.1.1
L333V
ratio of turnover number to Km-value: 238fold decrease with cis,cis-muconate as substrate, 41.5fold decrease with 2-chloro-cis,cis-muconate as substrate, 3.4fold increase with 3-chloro-cis,cis-muconate as substrate, 5.9fold decrease with 2,4-dichloro-cis,cis-muconate as substrate, 1.4fold decrease with 3-fluoro-cis,cis-muconate as substrate, 34.6fold decrease with 2-methyl-cis,cis-muconate as substrate, 16.5fold decrease with 3-methyl-cis,cis-muconate as substrate
Pseudomonas putida
5.5.1.1
Y59W
ratio of turnover number to Km-value: 12.9fold decrease with cis,cis-muconate as substrate, nearly identical to wild-type value with 2-chloro-cis,cis-muconate as substrate, 4.4fold decrease with 3-fluoro-cis,cis-muconate, 1.8fold decrease with 2-methyl-cis,cis-muconate as substrate, 10.2fold decrease with 3-methyl-cis,cis-muconate as substrate
Pseudomonas putida
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.5.1.1
2,4-dichloro-cis,cis-muconate
inhibitor of cis,cis-muconate conversion
Pseudomonas putida
5.5.1.1
2,4-dichloro-cis,cis-muconate
inhibitor of cis,cis-muconate conversion
Acinetobacter calcoaceticus
5.5.1.1
2-fluoro-cis,cis-muconate
inhibitor of cis,cis-muconate conversion
Pseudomonas putida
5.5.1.1
2-fluoro-cis,cis-muconate
inhibitor of cis,cis-muconate conversion
Acinetobacter calcoaceticus
5.5.1.1
3-chloro-cis,cis-muconate
inhibitor of cis,cis-muconate conversion
Pseudomonas putida
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
5.5.1.1
0.0035
-
2,4-dichloro-cis,cis-muconate
inhibitor of cis,cis-muconate conversion
Pseudomonas putida
5.5.1.1
0.027
-
2-fluoro-cis,cis-muconate
inhibitor of cis,cis-muconate conversion
Acinetobacter calcoaceticus
5.5.1.1
0.031
-
2-fluoro-cis,cis-muconate
inhibitor of cis,cis-muconate conversion
Pseudomonas putida
5.5.1.1
0.035
-
2,4-dichloro-cis,cis-muconate
inhibitor of cis,cis-muconate conversion
Acinetobacter calcoaceticus
5.5.1.1
0.095
-
3-chloro-cis,cis-muconate
inhibitor of cis,cis-muconate conversion
Pseudomonas putida
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.5.1.1
0.006
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
0.0064
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S or I54V/F329
Pseudomonas putida
5.5.1.1
0.0072
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
0.0091
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
0.0097
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S
Pseudomonas putida
5.5.1.1
0.012
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
0.015
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme Y59W
Pseudomonas putida
5.5.1.1
0.016
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme F329I
Pseudomonas putida
5.5.1.1
0.017
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme Y59W
Pseudomonas putida
5.5.1.1
0.026
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
0.033
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V/F329I
Pseudomonas putida
5.5.1.1
0.034
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
0.035
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S
Pseudomonas putida
5.5.1.1
0.036
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
0.037
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme F329I
Pseudomonas putida
5.5.1.1
0.039
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
0.04
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
0.04
-
cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
0.04
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
0.044
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S
Pseudomonas putida
5.5.1.1
0.045
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
0.045
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V/F329I
Pseudomonas putida
5.5.1.1
0.047
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
0.047
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme Y59W and F329I
Pseudomonas putida
5.5.1.1
0.052
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
0.057
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S or I54V/F329
Pseudomonas putida
5.5.1.1
0.058
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
0.059
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S
Pseudomonas putida
5.5.1.1
0.059
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
0.06
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S
Pseudomonas putida
5.5.1.1
0.063
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
0.068
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme F329I
Pseudomonas putida
5.5.1.1
0.069
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme Y59W
Pseudomonas putida
5.5.1.1
0.074
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
0.074
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
0.076
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
0.078
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme F329I
Pseudomonas putida
5.5.1.1
0.093
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
0.096
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
0.097
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
0.099
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C
Acinetobacter calcoaceticus
5.5.1.1
0.1
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C
Acinetobacter calcoaceticus
5.5.1.1
0.1
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S or I54V/F329
Pseudomonas putida
5.5.1.1
0.11
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
0.13
-
cis,cis-muconate
pH 7.5, 25°C
Acinetobacter calcoaceticus
5.5.1.1
0.14
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V/F329I
Pseudomonas putida
5.5.1.1
0.15
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme F329I
Pseudomonas putida
5.5.1.1
0.15
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme Y59W
Pseudomonas putida
5.5.1.1
0.18
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S
Pseudomonas putida
5.5.1.1
0.18
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
0.21
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V/F329I
Pseudomonas putida
5.5.1.1
0.23
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
0.29
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C
Acinetobacter calcoaceticus
5.5.1.1
0.32
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
0.49
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C
Acinetobacter calcoaceticus
5.5.1.1
0.55
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
0.69
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme F329I
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5.5.1.1
cis,cis-muconate
Pseudomonas putida
the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone
muconolactone
-
Pseudomonas putida
?
5.5.1.1
cis,cis-muconate
Acinetobacter calcoaceticus
the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone
muconolactone
-
Acinetobacter calcoaceticus
?
5.5.1.1
cis,cis-muconate
Pseudomonas putida PRS2000
the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone
muconolactone
-
Pseudomonas putida PRS2000
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.5.1.1
-
Pseudomonas putida
5.5.1.1
-
Acinetobacter calcoaceticus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5.5.1.1
2,4-dichloro-cis,cis-muconate
-
649348
Pseudomonas putida
?
-
649348
Pseudomonas putida
?
5.5.1.1
2,4-dichloro-cis,cis-muconate
-
649348
Pseudomonas putida PRS2000
?
-
649348
Pseudomonas putida PRS2000
?
5.5.1.1
2-Chloro-cis,cis-muconate
-
649348
Acinetobacter calcoaceticus
?
-
649348
Acinetobacter calcoaceticus
?
5.5.1.1
2-chloro-cis,cis-muconate
-
649348
Pseudomonas putida
(+)-2-chloromuconolactone + (+)-5-chloromuconolactone
mutant enzymes I54V and I54V/Y59W form 5-chloromuconolactone almost exclusively. Mutant enzymes F329I and Y59W form 2-chloromuconolactone as the main product. Mutant enzyme A271S hehaves similar to wild-type enzyme
649348
Pseudomonas putida
?
5.5.1.1
2-Methyl-cis,cis-muconate
-
649348
Acinetobacter calcoaceticus
?
-
649348
Acinetobacter calcoaceticus
?
5.5.1.1
2-methyl-cis,cis-muconate
-
649348
Pseudomonas putida
2-methylmuconolactone
-
649348
Pseudomonas putida
?
5.5.1.1
3-chloro-cis,cis-muconate
-
649348
Pseudomonas putida
protoanemonin + ?
formed by wild-type enzyme and mutant enzymes I54V, A271S, K276N, F329I, L333V, I54V/F329I
649348
Pseudomonas putida
?
5.5.1.1
3-Fluoro-cis,cis-muconate
-
649348
Pseudomonas putida
?
-
649348
Pseudomonas putida
?
5.5.1.1
3-Fluoro-cis,cis-muconate
-
649348
Acinetobacter calcoaceticus
?
-
649348
Acinetobacter calcoaceticus
?
5.5.1.1
3-Fluoro-cis,cis-muconate
-
649348
Pseudomonas putida PRS2000
?
-
649348
Pseudomonas putida PRS2000
?
5.5.1.1
3-Methyl-cis,cis-muconate
-
649348
Pseudomonas putida
?
-
649348
Pseudomonas putida
?
5.5.1.1
3-Methyl-cis,cis-muconate
-
649348
Acinetobacter calcoaceticus
?
-
649348
Acinetobacter calcoaceticus
?
5.5.1.1
3-Methyl-cis,cis-muconate
-
649348
Pseudomonas putida PRS2000
?
-
649348
Pseudomonas putida PRS2000
?
5.5.1.1
cis,cis-muconate
-
649348
Pseudomonas putida
muconolactone
-
649348
Pseudomonas putida
?
5.5.1.1
cis,cis-muconate
-
649348
Acinetobacter calcoaceticus
muconolactone
-
649348
Acinetobacter calcoaceticus
?
5.5.1.1
cis,cis-muconate
the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone
649348
Pseudomonas putida
muconolactone
-
649348
Pseudomonas putida
?
5.5.1.1
cis,cis-muconate
the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone
649348
Acinetobacter calcoaceticus
muconolactone
-
649348
Acinetobacter calcoaceticus
?
5.5.1.1
cis,cis-muconate
-
649348
Pseudomonas putida PRS2000
muconolactone
-
649348
Pseudomonas putida PRS2000
?
5.5.1.1
cis,cis-muconate
the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone
649348
Pseudomonas putida PRS2000
muconolactone
-
649348
Pseudomonas putida PRS2000
?
5.5.1.1
additional information
no activity with 3-chloro-cis,cis-muconate and 2,4-dichloro-cis,cis-muconate
649348
Acinetobacter calcoaceticus
?
-
649348
Acinetobacter calcoaceticus
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.5.1.1
0.4
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
3.4
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
36.6
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
43.8
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
52.8
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
174
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S
Pseudomonas putida
5.5.1.1
192
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
198
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
432
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
600
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V/F329I
Pseudomonas putida
5.5.1.1
900
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme Y59W and A271S
Pseudomonas putida
5.5.1.1
1020
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C
Acinetobacter calcoaceticus
5.5.1.1
1320
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V/F329I
Pseudomonas putida
5.5.1.1
1920
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme F329I
Pseudomonas putida
5.5.1.1
2100
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
2880
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme F329I
Pseudomonas putida
5.5.1.1
2940
-
2-chloro-cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
3120
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
3240
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
3840
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
4080
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
5700
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
6600
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V/F329I
Pseudomonas putida
5.5.1.1
8400
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme Y59W
Pseudomonas putida
5.5.1.1
12000
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
16200
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme F329I
Pseudomonas putida
5.5.1.1
18600
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S or I54V/F329
Pseudomonas putida
5.5.1.1
19200
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
22800
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
27600
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme F329I
Pseudomonas putida
5.5.1.1
32400
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V/F329I
Pseudomonas putida
5.5.1.1
35400
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
39000
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
40800
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S
Pseudomonas putida
5.5.1.1
44400
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
57600
-
3-chloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S
Pseudomonas putida
5.5.1.1
66000
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme Y59W
Pseudomonas putida
5.5.1.1
72000
-
2,4-dichloro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
84000
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S or I54V/F329
Pseudomonas putida
5.5.1.1
108000
-
3-methyl-cis,cis-muconate
pH 7.5, 25°C
Acinetobacter calcoaceticus
5.5.1.1
126000
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
150000
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V
Pseudomonas putida
5.5.1.1
156000
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C
Acinetobacter calcoaceticus
5.5.1.1
156000
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme F329I
Pseudomonas putida
5.5.1.1
192000
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme Y59W
Pseudomonas putida
5.5.1.1
202000
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C
Acinetobacter calcoaceticus
5.5.1.1
222000
-
cis,cis-muconate
pH 7.5, 25°C
Acinetobacter calcoaceticus
5.5.1.1
222000
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme Y59W
Pseudomonas putida
5.5.1.1
234000
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
246000
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme L333V
Pseudomonas putida
5.5.1.1
294000
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme I54V/F329I
Pseudomonas putida
5.5.1.1
304000
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S
Pseudomonas putida
5.5.1.1
312000
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N
Pseudomonas putida
5.5.1.1
360000
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S
Pseudomonas putida
5.5.1.1
426000
-
cis,cis-muconate
pH 7.5, 25°C, mutant enzyme A271S
Pseudomonas putida
5.5.1.1
480000
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme F329I
Pseudomonas putida
5.5.1.1
534000
-
3-fluoro-cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida
5.5.1.1
600000
-
2-methyl-cis,cis-muconate
pH 7.5, 25°C, mutant enzyme K276N or F329I
Pseudomonas putida
5.5.1.1
756000
-
cis,cis-muconate
pH 7.5, 25°C, wild-type enzyme
Pseudomonas putida