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Literature summary extracted from

  • Takakura, T.; Mitsushima, K.; Yagi, S.; Inagaki, K.; Tanaka, H.; Esaki, N.; Soda, K.; Takimoto, A.
    Assay method for antitumor L-methionine gamma-lyase: comprehensive kinetic analysis of the complex reaction with L-methionine (2004), Anal. Biochem., 327, 233-240.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.11 expression in Escherichia coli Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.11 0.2
-
 L-ethionine 37°C, pH 8.0 Pseudomonas putida
4.4.1.11 1.7
-
 L-methionine 37°C, pH 8.0 Pseudomonas putida
4.4.1.11 30
-
 L-homoserine 37°C, pH 8.0 Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.11 L-methionine + H2O Pseudomonas putida
-
 methanethiol + NH3 + 2-oxobutanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.11 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.11 recombinant METase Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.11 57
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.11 L-ethionine + H2O 60% of activity with L-methinine Pseudomonas putida ethanethiol + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 L-homoserine
-
 Pseudomonas putida NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 L-methionine + H2O
-
 Pseudomonas putida methanethiol + NH3 + 2-oxobutanoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.11 METase
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.11 pyridoxal 5'-phosphate
-
 Pseudomonas putida