Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gegg, M.E.; Clark, J.B.; Heales, S.J.R.
    Determination of glutamate-cysteine ligase (gamma-glutamylcysteine synthetase) activity by high-performance liquid chromatography and electrochemical detection (2002), Anal. Biochem., 304, 26-32.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.2 GSH
-
 Rattus norvegicus
6.3.2.2 L-buthionine-S-sulfoximine specific inhibitor Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.2 partially Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.2 astrocyte primary cell culture Rattus norvegicus
-
6.3.2.2 cell culture astrocyte, primary Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.2 0.0097
-
 partially purified enzyme from astrocytes Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.2 ATP + L-Glu + L-Cys
-
 Rattus norvegicus ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.2 gamma-GCS
-
 Rattus norvegicus
6.3.2.2 gamma-Glutamylcysteine synthetase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.2 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.2 8.2
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.2 ATP
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.2.2 0.1
-
 L-buthionine-S-sulfoximine about, pH 8.2, 37°C Rattus norvegicus
6.3.2.2 8.2
-
 GSH pH 8.2, 37°C Rattus norvegicus