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Literature summary extracted from

  • Honzatko, R.B.; Stayton, M.M.; Fromm, H.J.
    Adenylosuccinate synthetase: Recent developments (1999), Adv. Enzymol. Relat. Areas Mol. Biol., 73, 57-102.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.4 cDNA Dictyostelium discoideum
6.3.4.4 gene ade2 Schizosaccharomyces pombe
6.3.4.4 mouse muscle gene AdSS1 and mouse nonmuscle gene AdSS2 Mus musculus
6.3.4.4 purA gene Acidithiobacillus ferrooxidans
6.3.4.4 purA gene Haemophilus influenzae
6.3.4.4 purA gene Bacillus subtilis
6.3.4.4 purA gene overexpressed in strain H1238 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.4.4
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.4 R143K site-directed mutagenesis Escherichia coli
6.3.4.4 R143L site-directed mutagenesis Escherichia coli
6.3.4.4 R303L site-directed mutagenesis Escherichia coli
6.3.4.4 R304L site-directed mutagenesis Escherichia coli
6.3.4.4 R305L site-directed mutagenesis Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.4 Hadacidin
-
 Escherichia coli
6.3.4.4 Hadacidin
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.4 0.01
-
 GTP
-
 Escherichia coli
6.3.4.4 0.01
-
 L-aspartate
-
 Escherichia coli
6.3.4.4 0.02
-
 IMP
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.4 Ba2+ absolute requirement for divalent metal ions Escherichia coli
6.3.4.4 Ca2+ absolute requirement for divalent metal ions Escherichia coli
6.3.4.4 Co2+ absolute requirement for divalent metal ions Escherichia coli
6.3.4.4 Cu2+ absolute requirement for divalent metal ions Escherichia coli
6.3.4.4 Mg2+ absolute requirement for divalent metal ions, best activator Escherichia coli
6.3.4.4 Mn2+ absolute requirement for divalent metal ions Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.4 46600
-
 2 * 46600 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.4 GTP + IMP + L-aspartate Acidithiobacillus ferrooxidans first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate Pyrococcus sp. first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate Haemophilus influenzae first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate Bacillus subtilis first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate Mus musculus first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate Escherichia coli first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate Homo sapiens first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate Rattus norvegicus first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate Triticum aestivum first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate Oryctolagus cuniculus first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate Zea mays first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate Arabidopsis thaliana first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate Dictyostelium discoideum first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate Schizosaccharomyces pombe first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate Leishmania donovani first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate Azotobacter vinelandii first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate Trypanosoma cruzi first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate Methanocaldococcus jannaschii first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate Pyrococcus sp. ST700 first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.4 Acidithiobacillus ferrooxidans
-
-
-
6.3.4.4 Arabidopsis thaliana
-
-
-
6.3.4.4 Azotobacter vinelandii
-
-
-
6.3.4.4 Bacillus subtilis
-
-
-
6.3.4.4 Dictyostelium discoideum
-
-
-
6.3.4.4 Escherichia coli
-
-
-
6.3.4.4 Haemophilus influenzae
-
-
-
6.3.4.4 Homo sapiens
-
 human
-
6.3.4.4 Leishmania donovani
-
-
-
6.3.4.4 Methanocaldococcus jannaschii
-
-
-
6.3.4.4 Mus musculus
-
 mouse
-
6.3.4.4 Oryctolagus cuniculus
-
 rabbit
-
6.3.4.4 Pyrococcus sp.
-
-
-
6.3.4.4 Pyrococcus sp. ST700
-
-
-
6.3.4.4 Rattus norvegicus
-
 rat
-
6.3.4.4 Schizosaccharomyces pombe
-
-
-
6.3.4.4 Triticum aestivum
-
 wheat
-
6.3.4.4 Trypanosoma cruzi
-
-
-
6.3.4.4 Zea mays
-
 maize
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.4
-
 Rattus norvegicus
6.3.4.4
-
 Oryctolagus cuniculus
6.3.4.4
-
 Dictyostelium discoideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.4 GTP + IMP + L-aspartate
-
 Acidithiobacillus ferrooxidans GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate
-
 Pyrococcus sp. GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate
-
 Haemophilus influenzae GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate
-
 Bacillus subtilis GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate
-
 Mus musculus GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate
-
 Escherichia coli GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate
-
 Homo sapiens GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate
-
 Rattus norvegicus GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate
-
 Triticum aestivum GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate
-
 Oryctolagus cuniculus GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate
-
 Zea mays GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate
-
 Arabidopsis thaliana GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate
-
 Dictyostelium discoideum GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate
-
 Schizosaccharomyces pombe GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate
-
 Leishmania donovani GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate
-
 Azotobacter vinelandii GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate
-
 Trypanosoma cruzi GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate
-
 Methanocaldococcus jannaschii GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Acidithiobacillus ferrooxidans GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Pyrococcus sp. GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Haemophilus influenzae GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Bacillus subtilis GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Mus musculus GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Escherichia coli GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Homo sapiens GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Rattus norvegicus GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Triticum aestivum GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Oryctolagus cuniculus GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Zea mays GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Arabidopsis thaliana GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Dictyostelium discoideum GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Schizosaccharomyces pombe GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Leishmania donovani GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Azotobacter vinelandii GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Trypanosoma cruzi GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Methanocaldococcus jannaschii GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate
-
 Pyrococcus sp. ST700 GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Pyrococcus sp. ST700 GDP + phosphate + adenylosuccinate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.4 homodimer 2 * 46600 Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
6.3.4.4 adenosylsuccinate synthetase
-
 Escherichia coli
6.3.4.4 adenosylsuccinate synthetase
-
 Rattus norvegicus
6.3.4.4 Adenylosuccinate synthetase
-
 Acidithiobacillus ferrooxidans
6.3.4.4 Adenylosuccinate synthetase
-
 Pyrococcus sp.
6.3.4.4 Adenylosuccinate synthetase
-
 Haemophilus influenzae
6.3.4.4 Adenylosuccinate synthetase
-
 Bacillus subtilis
6.3.4.4 Adenylosuccinate synthetase
-
 Mus musculus
6.3.4.4 Adenylosuccinate synthetase
-
 Homo sapiens
6.3.4.4 Adenylosuccinate synthetase
-
 Triticum aestivum
6.3.4.4 Adenylosuccinate synthetase
-
 Oryctolagus cuniculus
6.3.4.4 Adenylosuccinate synthetase
-
 Zea mays
6.3.4.4 Adenylosuccinate synthetase
-
 Arabidopsis thaliana
6.3.4.4 Adenylosuccinate synthetase
-
 Dictyostelium discoideum
6.3.4.4 Adenylosuccinate synthetase
-
 Schizosaccharomyces pombe
6.3.4.4 Adenylosuccinate synthetase
-
 Leishmania donovani
6.3.4.4 Adenylosuccinate synthetase
-
 Azotobacter vinelandii
6.3.4.4 Adenylosuccinate synthetase
-
 Trypanosoma cruzi
6.3.4.4 Adenylosuccinate synthetase
-
 Methanocaldococcus jannaschii
6.3.4.4 AMPSase
-
 Acidithiobacillus ferrooxidans
6.3.4.4 AMPSase
-
 Pyrococcus sp.
6.3.4.4 AMPSase
-
 Haemophilus influenzae
6.3.4.4 AMPSase
-
 Bacillus subtilis
6.3.4.4 AMPSase
-
 Mus musculus
6.3.4.4 AMPSase
-
 Escherichia coli
6.3.4.4 AMPSase
-
 Homo sapiens
6.3.4.4 AMPSase
-
 Rattus norvegicus
6.3.4.4 AMPSase
-
 Triticum aestivum
6.3.4.4 AMPSase
-
 Oryctolagus cuniculus
6.3.4.4 AMPSase
-
 Zea mays
6.3.4.4 AMPSase
-
 Arabidopsis thaliana
6.3.4.4 AMPSase
-
 Dictyostelium discoideum
6.3.4.4 AMPSase
-
 Schizosaccharomyces pombe
6.3.4.4 AMPSase
-
 Leishmania donovani
6.3.4.4 AMPSase
-
 Azotobacter vinelandii
6.3.4.4 AMPSase
-
 Trypanosoma cruzi
6.3.4.4 AMPSase
-
 Methanocaldococcus jannaschii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.4.4 1.4
-
 GTP
-
 Escherichia coli

pI Value

EC Number Organism Comment pI Value Maximum pI Value
6.3.4.4 Rattus norvegicus acidic isoenzyme
-
 5.9
6.3.4.4 Rattus norvegicus basic isoenzyme
-
 8.9
6.3.4.4 Mus musculus most abundant isozyme
-
 8.9