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Literature summary extracted from

  • Jeong, M.S.; Jeong, J.K.; Park, K.S.; Kim, H.T.; Lee, K.M.; Lim, W.K.; Jang, S.B.
    Crystallization and preliminary X-ray analysis of tryptophan synthase alpha-subunits from Escherichia coli (2004), Acta Crystallogr. Sect. D, 60, 132-134.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.20 overexpression of the alpha-subunit in strain RB797 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.20 10 mg/ml purified recombinant alpha-subunit, hanging drop vapour diffusion method, 298K, equal volume, 0.001 ml, of protein solution and reservoir solution are mixed and placed over 0.5 ml reservoir solution, precipitant solution: 0.5 M ammonium sulfate, 0.1 M trisodium citrate dihydrate, 1.0 M lithium sulfate monohydrate, pH 5.6, first crystals after 7-10 days, maximal size within 2 weeks, X-ray diffraction structure determination and analysis at 2.8 A resolution Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.20 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.20 recombinant alpha-subunit Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.20 L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8) Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.20 1-(indol-3-yl)glycerol 3-phosphate alpha-subunit of the bienzyme complex, alpha-reaction Escherichia coli D-glyceraldehyde 3-phosphate + indole channeling of indole to the beta-subunit active site ?
4.2.1.20 L-serine + indole beta-subunit of the bienzyme complex, beta-reaction Escherichia coli L-tryptophan + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.20 More the alpha-subunit contains no disulfide bond, conformational stabilization mechanism Escherichia coli
4.2.1.20 tetramer alpha2beta2 enzyme complex Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
4.2.1.20 alphaTS alpha-subunit Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.20 additional information the alpha-subunit contains no prosthetic group Escherichia coli