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Literature summary extracted from
- The dual role of CHAPS in the crystallization of stromelysin-3 catalytic domain (2003), Acta Crystallogr. Sect. D, 59, 603-606.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.24.B3 | expression in Escherichia coli BL21(DE3) | Mus musculus |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.4.24.B3 | enzyme complexed with a phosphinic inhibitor, inhibitor to protein ratio is 2.2:1, in presence of detergent CHAPS, hanging drop method, 5 mg/ml protein in 0.05 M MES, pH 5.5, 0.5 M ammonium sulfate, 1% v/v trifluoroethanol, X-ray diffraction structure determination at 2.6 A resolution and analysis | Mus musculus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.B3 | phosphinic inhibitor | - |
Mus musculus |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.B3 | Zn2+ | zinc binding motif H219EXXHXGXXH229 | Mus musculus |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.B3 | protein + H2O | Mus musculus | - |
peptides | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.B3 | Mus musculus | Q02853 | precursor | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.24.B3 | recombinant from Escherichia coli, addition of CHAPS, i.e. 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate, to avoid protein aggregation, binding mechanism | Mus musculus |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 3.4.24.B3 | renaturation from inclusion bodies after expression in Escherichia coli | Mus musculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.B3 | protein + H2O | - |
Mus musculus | peptides | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.24.B3 | M10.007 | Merops-ID | Mus musculus |
| 3.4.24.B3 | matrix metalloproteinase-11 | - |
Mus musculus |
| 3.4.24.B3 | MMP-11 | - |
Mus musculus |
| 3.4.24.B3 | ST3 | - |
Mus musculus |
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Release 2023.1 (January 2023)
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