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Literature summary extracted from

  • Koski, M.K.; Haapalainen, A.M.; Hiltunen, J.K.; Glumoff, T.
    Crystallization and preliminary crystallographic data of 2-enoyl-CoA hydratase 2 domain of Candida tropicalis peroxisomal multifunctional enzyme type 2 (2003), Acta Crystallogr. Sect. D, 59, 1302-1305.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.119 expressed in Escherichia coli BL21(DE3) Candida tropicalis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.119 hanging-drop vapour-diffusion method. Crystals of native and SeMet CtMfe2p(dha+bdelta) Candida tropicalis

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.119 additional information CtMfe2p(dha+bdelta) labelled with selenomethionine (SeMet), the plasmid pET3a::CtMfe2p(dha+bdelta) is transformed to the methionine-auxotrophic Escherichia coli strain B834(DE3). The incorporation of SeMet into the structure does not affect the hydratase 2 activity Candida tropicalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.119 63000
-
 both recombinant CtMfe2p(dha+bdelta) and its SeMet analogue, SDS-PAGE Candida tropicalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.119 additional information Candida tropicalis in yeast, the second and the third reaction of the fatty-acid beta-oxidation spiral are catalysed by peroxisomal multifunctional enzyme type 2 (Mfe2p/Fox2p). This protein has two (3R)-hydroxyacyl-CoA dehydrogenase domains and a C-terminal 2-enoyl-CoA hydratase 2 domain ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.119 Candida tropicalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.119
-
 Candida tropicalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.119 (2E)-2-decenoyl-CoA + H2O activity measurements are based on the formation of the magnesium complex of 3-ketoacyl-CoA from (2E)-2-decenoyl-CoA Candida tropicalis (3R)-3-hydroxydecanoyl-CoA
-
 ?
4.2.1.119 additional information in yeast, the second and the third reaction of the fatty-acid beta-oxidation spiral are catalysed by peroxisomal multifunctional enzyme type 2 (Mfe2p/Fox2p). This protein has two (3R)-hydroxyacyl-CoA dehydrogenase domains and a C-terminal 2-enoyl-CoA hydratase 2 domain Candida tropicalis ?
-
 ?
4.2.1.119 additional information MFE-2 is a multifunctional enzyme with 2-enoyl-CoA hydratase 2 activity and 2/(3R)-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.36) activity Candida tropicalis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.119 ? x * 63000, both recombinant CtMfe2p(dha+bdelta) and its SeMet analogue, SDS-PAGE Candida tropicalis

Synonyms

EC Number Synonyms Comment Organism
4.2.1.119 2-enoyl-CoA hydratase 2
-
 Candida tropicalis
4.2.1.119 MFE-2 multifunctional enzyme Candida tropicalis
4.2.1.119 MFE-2 multifunctional enzyme with 2-enoyl-CoA hydratase 2 activity and 2/(3R)-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.36) activity Candida tropicalis
4.2.1.119 Mfe2p [CtMfe2p(dha+bdelta)] 2-enoyl-CoA hydratase 2 domain of Candida tropicalis Candida tropicalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.119 388
-
 (2E)-2-decenoyl-CoA recombinant enzyme CtMfe2p(dha+bdelta) Candida tropicalis