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Literature summary extracted from

  • Hibi, T.; Hisada, H.; Nakatsu, T.; Kato, H.; Oda, J.
    Escherichia coli B gamma-glutamylcysteine synthetase: modification, purification, crystallization and preliminary crystallographic analysis (2002), Acta Crystallogr. Sect. D, 58, 316-318.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.3.2.2 medicine the enzyme is a target for development of potential therapeutic agents Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.2 expression of wild-type and mutant enzymes in Escherichia coli JM109 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.2.2 22.6 mg/ml purified enzyme, wild-type or mutant, hanging drop vapour diffusion method, equal volume of protein and reservoir solution, reservoir solution: 3.9 M sodium formate, pH 7.4, equilibration with reservoir solution at 293K, X-ray diffraction structure determination and analysis at 2.8 A resolution Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.3.2.2 C106S site-directed mutagenesis, exchange of surface exposed cysteine residue for improved crystallization Escherichia coli
6.3.2.2 C164S site-directed mutagenesis, exchange of surface exposed cysteine residue for improved crystallization Escherichia coli
6.3.2.2 C205S site-directed mutagenesis, exchange of surface exposed cysteine residue for improved crystallization Escherichia coli
6.3.2.2 C223S site-directed mutagenesis, exchange of surface exposed cysteine residue for improved crystallization Escherichia coli
6.3.2.2 additional information construction of a quadruple mutant of the enzyme termed gamma-GCS4CS Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.2 ATP + L-Glu + L-Cys Escherichia coli catalyzes the biosynthesis of the GSH precursor ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
6.3.2.2 ATP + L-Glu + L-Cys Escherichia coli B / ATCC 11303 catalyzes the biosynthesis of the GSH precursor ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.2 Escherichia coli
-
 B
-
6.3.2.2 Escherichia coli B / ATCC 11303
-
 B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.2 recombinant wild-type and mutant enzymes from Escherichia coli Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.2 ATP + L-Glu + L-Cys
-
 Escherichia coli ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
6.3.2.2 ATP + L-Glu + L-Cys catalyzes the biosynthesis of the GSH precursor Escherichia coli ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
6.3.2.2 ATP + L-Glu + L-Cys
-
 Escherichia coli B / ATCC 11303 ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
6.3.2.2 ATP + L-Glu + L-Cys catalyzes the biosynthesis of the GSH precursor Escherichia coli B / ATCC 11303 ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.2 gamma-GCS
-
 Escherichia coli
6.3.2.2 gamma-Glutamylcysteine synthetase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.2 ATP
-
 Escherichia coli