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Literature summary extracted from

  • Sridhar, V.; Xu, M.; Han, Q.; Sun, X.; Tan, Y.; Hoffman, R.M.; Prasad, G.S.
    Crystallization and preliminary crystallographic characterization of recombinant L-methionine-alpha-deamino-gamma-mercaptomethane lyase (methioninase) (2000), Acta Crystallogr. Sect. D, 56, 1665-1667.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.11 expression in Escherichia coli Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.4.1.11 sitting-drop vapor diffusion, 1.25 M ammonium sulfate in 100 mM MES-HCl, pH 6.0 as reservoir solution, protein solution consists of 10-20 mg/ml METase in 120 mM sodium chloride, 10 mM sodium phosphate, pH 7.2, crystals diffract to at least 2.68 A Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.11 L-methionine + H2O Pseudomonas putida
-
 methanethiol + NH3 + 2-oxobutanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.11 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.11 recombinant rMETase Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.11 L-methionine + H2O
-
 Pseudomonas putida methanethiol + NH3 + 2-oxobutanoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.11 rMETase
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.11 pyridoxal 5'-phosphate
-
 Pseudomonas putida