Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gilboa, R.; Greenblatt, H.M.; Perach, M.; Spungin-Bialik, A.; Lessel, U.; Wohlfahrt, G.; Schomburg, D.; Blumberg, S.; Shoham, G.
    Interactions of Streptomyces griseus aminopeptidase with a methionine product analogue: a structural study at 1.53 A resolution (2000), Acta Crystallogr. Sect. D, D56, 551-558.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.11.24 biotechnology the enzyme is useful in many biotechnological applications e.g. in processing of recombinant DNA, proteins, and fusion protein products Streptomyces griseus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.11.24 purified enzyme complexed with methionine, hanging-drop vapour diffusion method, protein solution: 18 mg/ml protein, 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 6 mM CaCl2, 0.1 M methionine, plus equal volume of precipitant solution: 24% w/v PEG 4000, 0.1 M ammonium sulfate, equilibrated against 1 ml reservoir of the precipitant solution, 3-4 days, X-ray diffraction structure determination at 1.53 A high resolution and analysis Streptomyces griseus
3.4.11.24 purified native enzyme in complex with product analogous weak inhibiting amino acids phenylalanine, leucine, and methionine, hanging drop vapour diffusion method, 18 mg/ml protein in 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 6 mM CaCl2, and 100 mM L-methionine or 200 mM L-leucine, the precipitant solution contains 24% w/v PEG 4000 and 0.1 M ammonium sulfate, equilibration against 1 ml reservoir solution, microseeding, 3-4 days, with phenylalanine a protein solution containing 18 mg/ml protein, 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 6 mM CaCl2, and 100 mM Phe is mixed with a reservoir solution containing 18% w/v PEG 4000, and 0.1 M acetate buffer, pH 5.5, microseeding, 3-4 days, X-ray diffraction structure determination and analysis at 1.8 A, 1.7 A, and 1.53 A resolution, respectively, structure modelling Streptomyces griseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.24 leucine weak inhibitor, binding structure, overview Streptomyces griseus
3.4.11.24 methionine weak inhibitor, binding structure, overview Streptomyces griseus
3.4.11.24 phenylalanine weak inhibitor, binding structure, overview Streptomyces griseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.24 extracellular
-
 Streptomyces griseus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.24 Ca2+ modulating the enzyme activity Streptomyces griseus
3.4.11.24 Zn2+ 2 mol zinc per mol of enzyme, binding structure Streptomyces griseus
3.4.11.24 Zn2+ the enzyme is a double-zinc exopeptidase, binding structure, overview Streptomyces griseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.24 30000
-
 1 * 30000 Streptomyces griseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.24 peptide + H2O Streptomyces griseus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.24 Streptomyces griseus
-
-
-
3.4.11.24 Streptomyces griseus P80561 purified enzyme
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.11.24 release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues catalytic mechanism, Asp160, Met161, Gly201, Arg202, and Phe219 are involved, active site structure, modeling of enzyme-substrate complex Streptomyces griseus
a
3.4.11.24 release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues the enzyme prefers large hydrophobic aminoterminal residues, Glu131, Asp160, and Arg202 are involved in binding of the N-terminal substrate amino acid, substrate binding and reaction mechanism, tetrahedral reaction intermediate, modelling of the enzyme-substrate and enzyme-product complexes Streptomyces griseus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.24 peptide + H2O
-
 Streptomyces griseus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.24 monomer 1 * 30000 Streptomyces griseus

Synonyms

EC Number Synonyms Comment Organism
3.4.11.24 M28.003 Merops-ID Streptomyces griseus
3.4.11.24 SGAP
-
 Streptomyces griseus
3.4.11.24 Streptomyces griseus aminopeptidase
-
 Streptomyces griseus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.24 additional information
-
 the enzyme is heat-stable Streptomyces griseus