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Literature summary extracted from

  • Szabelski, M.; Stachowiak, K.; Wiczk, W.
    Influence of organic solvents on papain kinetics (2001), Acta Biochim. Pol., 48, 1197-1201.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.2 dimethylformamide number of papain active sites decreases with increase of inhibitor concentration Carica papaya
3.4.22.2 methanol number of papain active sites decreases with increase of inhibitor concentration Carica papaya

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.2 0.00385
-
 Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans pH 6.8, 40°C, in presence of 1% methanol Carica papaya
3.4.22.2 0.01243
-
 Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans pH 6.8, 40°C, in presence of 5% methanol Carica papaya
3.4.22.2 0.02075
-
 Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans pH 6.8, 40°C, in presence of 0.6% dimethylformamide Carica papaya
3.4.22.2 1.765
-
 Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans pH 6.8, 40°C, in presence of 5% dimethylformamide Carica papaya

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.2 Carica papaya
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.2 Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans + H2O
-
 Carica papaya Dabcyl-Lys-Phe-Gly + Gly-Ala-Ala-Edans
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.2 0.03 0.55 Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans pH 6.8, 40°C, in presence of 5% methanol Carica papaya
3.4.22.2 0.41
-
 Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans pH 6.8, 40°C, in presence of 0.6% dimethylformamide Carica papaya
3.4.22.2 2.77
-
 Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans pH 6.8, 40°C, in presence of 1% methanol Carica papaya
3.4.22.2 3 6 Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans pH 6.8, 40°C, in presence of 1% methanol Carica papaya
3.4.22.2 6.86
-
 Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans pH 6.8, 40°C, in presence of 5% methanol Carica papaya