EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.3.3.13 | additional information | not inhibited by EDTA, o-phenanthroline, acetylsalicylic acid, baicalein, dipyridamole, eicosatetraynoic acid, esculetin, indomethacin, naproxen, NDGA or SKF-525A | Ptilota filicina |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.3.13 | 0.0328 | - |
(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate | pH 7.2 | Ptilota filicina | |
5.3.3.13 | 0.0399 | - |
(5Z,8Z,11Z,14Z)-eicosatetraenoate | pH 7.2 | Ptilota filicina | |
5.3.3.13 | 0.15 | - |
(6Z,9Z,12Z)-octadecatrienoate | pH 7.2 | Ptilota filicina |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.3.3.13 | 58119 | - |
3 * 61000, SDS-PAGE, 3 * 58119, mass spectrometry | Ptilota filicina |
5.3.3.13 | 61000 | - |
3 * 61000, SDS-PAGE, 3 * 58119, mass spectrometry | Ptilota filicina |
5.3.3.13 | 174000 | - |
gel filtration | Ptilota filicina |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.3.13 | (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate | Ptilota filicina | likely the native substrate, biosynthesis of conjugated triene-containing fatty acids | (5Z,7E,9E,14Z,17Z)-eicosapentaenoate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.3.13 | Ptilota filicina | - |
- |
- |
5.3.3.13 | Ptilota filicina | - |
temperate red alga from the Oregon coast | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.3.3.13 | 102fold | Ptilota filicina |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
5.3.3.13 | (5Z,8Z,11Z,14Z,17Z)-icosapentaenoate = (5Z,7E,9E,14Z,17Z)-icosapentaenoate | regio- and stereochemistry of the isomerization reaction, mechanism | Ptilota filicina |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.3.3.13 | 1.06 | 3.5 | pH 7.2, arachidonate as substrate | Ptilota filicina |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.3.13 | (5Z,8Z,11Z,14Z)-eicosatetraenoate | arachidonate, next best substrate after (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate, the C11 olefinic position becomes protonated by a solvent-derived proton, mechanism | Ptilota filicina | (5Z,7E,9E,14Z)-eicosatetraenoate | - |
? | |
5.3.3.13 | (5Z,8Z,11Z,14Z)-eicosatetraenoyl methyl ester | methyl ester of arachidonate | Ptilota filicina | (5Z,7E,9E,14Z,17Z)-eicosapentaenoyl methyl ester | - |
? | |
5.3.3.13 | (5Z,8Z,11Z,14Z)-eicosatetraenoyl-N-ethanolamide | arachidonoylethanolamide | Ptilota filicina | (5Z,7E,9E,14Z)-eicosatetraenoyl-N-ethanolamide | - |
? | |
5.3.3.13 | (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate | likely the native substrate, biosynthesis of conjugated triene-containing fatty acids | Ptilota filicina | (5Z,7E,9E,14Z,17Z)-eicosapentaenoate | - |
? | |
5.3.3.13 | (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate | best substrate, likely the native substrate, mechanism | Ptilota filicina | (5Z,7E,9E,14Z,17Z)-eicosapentaenoate | - |
? | |
5.3.3.13 | (6Z,9Z,12Z)-octadecatrienoate | gamma-linolenate, PFI intramolecularly transfers the bis-allylic pro-S hydrogen from the C11 position to the C13 position, the bis-allylic pro-R hydrogen at C8 in gamma-linolenate is lost to the solvent | Ptilota filicina | (6Z,8E,10E)-octadecatrienoate | - |
? | |
5.3.3.13 | (9Z,12Z,15Z)-octadecatrienoate | alpha-linolenate, one-fifth as fast as gamma-linolenate | Ptilota filicina | ? | - |
? | |
5.3.3.13 | additional information | not: linoleic acid | Ptilota filicina | ? | - |
? | |
5.3.3.13 | polyunstaurated fatty acid | PFI catalyzes the formation of conjugated trienes from a variety of polyunsaturated fatty acid precursors, regio- and stereochemistry, enzyme may recognize the protonated from of the substrate | Ptilota filicina | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.3.3.13 | trimer | 3 * 61000, SDS-PAGE, 3 * 58119, mass spectrometry | Ptilota filicina |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.3.13 | 41 | - |
(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate | pH 7.2, assuming one catalytic site per enzyme molecule | Ptilota filicina |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.3.3.13 | 4.5 | - |
- |
Ptilota filicina |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
5.3.3.13 | 4 | 8 | there is a 30% increase in rate of product formation from pH 8 to 6.5, from pH 6.5 to 4.5 the apparent velocity increases 2.4fold, below pH 4 the activity slowly decreases | Ptilota filicina |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
5.3.3.13 | additional information | no requirement for molecular oxygen | Ptilota filicina |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
5.3.3.13 | Ptilota filicina | isoelectric focusing | - |
4.5 |