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Literature summary extracted from

  • Bearne, S.L.; Hekmat, O.; Macdonnell, J.E.
    Inhibition of Escherichia coli CTP synthase by glutamate gamma-semialdehyde and the role of the allosteric effector GTP in glutamine hydrolysis (2001), Biochem. J., 356, 223-232.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.4.2 GTP required as allosteric effector to promote reaction with glutamine as substrate, GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.2 overexpression as a hexahistidine-tagged form Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.2 C379A mutant enzyme is fully active with ammonia but has no glutamine-dependent activity, no inhibition by glutamate gamma-semialdehyde Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.2 D,L-2-amino-4-phosphonobutyrate
-
 Escherichia coli
6.3.4.2 DELTA1-Pyrroline-5-carboxylate weak Escherichia coli
6.3.4.2 glutamate gamma-semialdehyde potent linear mixed-type inhibitor, competitive with respect to ammonia, no inhibition of the mutant enzyme C379A Escherichia coli
6.3.4.2 L-2-pyrrolidone 5-carboxylate weak competitive inhibition of the reaction with ammonia as substrate, no significant inhibition with glutamine as substrate Escherichia coli
6.3.4.2 additional information enzyme loses activity at ionic strengths higher than 0.4 M Escherichia coli
6.3.4.2 pyrrole-2-carboxylate weak competitive inhibition of the reaction with ammonia as substrate, no significant inhibition with glutamine as substrate Escherichia coli
6.3.4.2 SO42-
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.2 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.2
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.2 ATP + UTP + glutamine
-
 Escherichia coli ADP + phosphate + CTP + Glu
-
 ?
6.3.4.2 ATP + UTP + NH4+
-
 Escherichia coli ADP + phosphate + CTP
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.4.2 6.7
-
 glutamine pH 8.0, wild-type enzyme Escherichia coli
6.3.4.2 8.1
-
 glutamine pH 8.0, recombinant His6-tagged enzyme Escherichia coli
6.3.4.2 9.9
-
 NH3 pH 8.0, mutant enzyme C379A Escherichia coli
6.3.4.2 10.4
-
 NH3 pH 8.0, mutant enzyme C379S Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.4.2 additional information
-
 additional information
-
 Escherichia coli
6.3.4.2 0.39
-
 glutamate gamma-semialdehyde
-
 Escherichia coli
6.3.4.2 5.6
-
 D,L-2-amino-4-phosphonobutyrate pH 8.0, reaction with ammonia Escherichia coli
6.3.4.2 11
-
 pyrrole-2-carboxylate pH 8.0, reaction with ammonia Escherichia coli
6.3.4.2 12.6
-
 L-2-pyrrolidone-5-carboxylate pH 8.0, reaction with ammonia Escherichia coli