BRENDA - Enzyme Database

Phosphopantothenoylcysteine synthetase from Escherichia coli. Identification and characterization of the last unidentified coenzyme A biosynthetic enzyme in bacteria

Strauss, E.; Kinsland, C.; Ge, Y.; McLafferty, F.W.; Begley, T.P.; J. Biol. Chem. 276, 13513-13516 (2001) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
6.3.2.5
expressed in Escherichia coli Tuner(DE3) cells
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.2.5
0.24
-
cysteine
pH 7.6
Escherichia coli
6.3.2.5
0.24
-
L-cysteine
at pH 7.6 and 25°C
Escherichia coli
6.3.2.5
0.29
-
CTP
pH 7.6
Escherichia coli
6.3.2.5
0.29
-
4'-phosphopantothenate
pH 7.6
Escherichia coli
6.3.2.5
0.29
-
CTP
at pH 7.6 and 25°C
Escherichia coli
6.3.2.5
0.29
-
(R)-4'-phosphopantothenate
at pH 7.6 and 25°C
Escherichia coli
6.3.2.5
0.78
-
(R)-4'-phosphopantothenoyl-L-cysteine
pH 7.6
Escherichia coli
6.3.2.5
0.78
-
N-[(R)-4'-phosphopantothenoyl]-L-cysteine
at pH 7.6 and 25°C
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.2.5
45000
-
x * 45000, SDS-PAGE
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
6.3.2.5
CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
Escherichia coli
-
CTP + (R)-4'-phosphopantothenate + L-cysteine
-
-
r
6.3.2.5
CTP + (R)-4'-phosphopantothenate + L-cysteine
Escherichia coli
-
CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
-
r
Organism
EC Number
Organism
UniProt
Commentary
Textmining
6.3.2.5
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
6.3.2.5
ammonium sulfate precipitation, Sephacryl S-200 column chromatography, DEAE-Sepharose column chromatography, hydroxyapatite column chromatography, HiTrap Blue column chromatography, and Superdex 200 gel filtration
Escherichia coli
6.3.2.5
partial
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.3.2.5
0.371
-
after 1280fold purification, at pH 7.6 and 25°C
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
6.3.2.5
CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
648960
Escherichia coli
CTP + (R)-4'-phosphopantothenate + L-cysteine
-
-
-
r
6.3.2.5
CTP + (R)-4'-phosphopantothenate + L-Cys
CTP specifically required
648960
Escherichia coli
(R)-4'-phosphopantothenoyl-L-cysteine + CDP + phosphate
-
-
-
r
6.3.2.5
CTP + (R)-4'-phosphopantothenate + L-cysteine
-
648960
Escherichia coli
CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
-
-
r
6.3.2.5
additional information
ATP, GTP, and UTP cannot substitute for CTP
648960
Escherichia coli
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
6.3.2.5
?
x * 45000, SDS-PAGE
Escherichia coli
6.3.2.5
?
x * 45471, ESI/FTMS
Escherichia coli
6.3.2.5
More
enzyme is part of the Dfp flavoprotein
Escherichia coli
Synonyms
EC Number
Synonyms
Commentary
Organism
6.3.2.5
CoaBC
-
Escherichia coli
6.3.2.5
Dfp
-
Escherichia coli
6.3.2.5
phosphopantothenoylcysteine synthetase
-
Escherichia coli
6.3.2.5
PPC-S
-
Escherichia coli
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.3.2.5
0.83
-
N-[(R)-4'-phosphopantothenoyl]-L-cysteine
at pH 7.6 and 25°C
Escherichia coli
6.3.2.5
0.88
-
(R)-4'-phosphopantothenate
at pH 7.6 and 25°C
Escherichia coli
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
6.3.2.5
CTP
-
Escherichia coli
6.3.2.5
FMN
-
Escherichia coli
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.2.5
expressed in Escherichia coli Tuner(DE3) cells
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
6.3.2.5
CTP
-
Escherichia coli
6.3.2.5
FMN
-
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.2.5
0.24
-
cysteine
pH 7.6
Escherichia coli
6.3.2.5
0.24
-
L-cysteine
at pH 7.6 and 25°C
Escherichia coli
6.3.2.5
0.29
-
CTP
pH 7.6
Escherichia coli
6.3.2.5
0.29
-
4'-phosphopantothenate
pH 7.6
Escherichia coli
6.3.2.5
0.29
-
CTP
at pH 7.6 and 25°C
Escherichia coli
6.3.2.5
0.29
-
(R)-4'-phosphopantothenate
at pH 7.6 and 25°C
Escherichia coli
6.3.2.5
0.78
-
(R)-4'-phosphopantothenoyl-L-cysteine
pH 7.6
Escherichia coli
6.3.2.5
0.78
-
N-[(R)-4'-phosphopantothenoyl]-L-cysteine
at pH 7.6 and 25°C
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.2.5
45000
-
x * 45000, SDS-PAGE
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
6.3.2.5
CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
Escherichia coli
-
CTP + (R)-4'-phosphopantothenate + L-cysteine
-
-
r
6.3.2.5
CTP + (R)-4'-phosphopantothenate + L-cysteine
Escherichia coli
-
CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.2.5
ammonium sulfate precipitation, Sephacryl S-200 column chromatography, DEAE-Sepharose column chromatography, hydroxyapatite column chromatography, HiTrap Blue column chromatography, and Superdex 200 gel filtration
Escherichia coli
6.3.2.5
partial
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.3.2.5
0.371
-
after 1280fold purification, at pH 7.6 and 25°C
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
6.3.2.5
CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
648960
Escherichia coli
CTP + (R)-4'-phosphopantothenate + L-cysteine
-
-
-
r
6.3.2.5
CTP + (R)-4'-phosphopantothenate + L-Cys
CTP specifically required
648960
Escherichia coli
(R)-4'-phosphopantothenoyl-L-cysteine + CDP + phosphate
-
-
-
r
6.3.2.5
CTP + (R)-4'-phosphopantothenate + L-cysteine
-
648960
Escherichia coli
CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
-
-
r
6.3.2.5
additional information
ATP, GTP, and UTP cannot substitute for CTP
648960
Escherichia coli
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.3.2.5
?
x * 45000, SDS-PAGE
Escherichia coli
6.3.2.5
?
x * 45471, ESI/FTMS
Escherichia coli
6.3.2.5
More
enzyme is part of the Dfp flavoprotein
Escherichia coli
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.3.2.5
0.83
-
N-[(R)-4'-phosphopantothenoyl]-L-cysteine
at pH 7.6 and 25°C
Escherichia coli
6.3.2.5
0.88
-
(R)-4'-phosphopantothenate
at pH 7.6 and 25°C
Escherichia coli