Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Manoj, N.; Strauss, E.; Begley, T.P.; Ealick, S.E.
    Structure of human phosphopantothenoylcysteine synthetase at 2.3 A resolution (2003), Structure, 11, 927-936.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.51 expressed in Escherichia coli B834(DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.36
-
 Homo sapiens
6.3.2.5
-
 Homo sapiens
6.3.2.51 hanging drop vapor diffusion method, using 24% polyethyleneglycol monomethyl ether 2000, 120 mM (NH4)2SO4, and 100 mM Tris-HCl (pH 8.5) Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.51 ATP + (R)-4'-phosphopantothenate + L-cysteine Homo sapiens
-
 AMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.36 Homo sapiens
-
 human
-
6.3.2.5 Homo sapiens
-
-
-
6.3.2.51 Homo sapiens Q9HAB8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.51 Ni-NTA resin column chromatography Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.2.5 CTP + (R)-4'-phosphopantothenate + L-cysteine = CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine ping pong mechanism Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.5 ATP + (R)-4'-phosphopantothenate + L-Cys
-
 Homo sapiens (R)-4'-phosphopantothenoyl-L-cysteine + ADP + phosphate
-
 ?
6.3.2.51 ATP + (R)-4'-phosphopantothenate + L-cysteine
-
 Homo sapiens AMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.36 homodimer
-
 Homo sapiens
6.3.2.5 dimer two identical subunits, crystallization data Homo sapiens
6.3.2.51 homodimer x-ray crystallography Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
4.1.1.36 PPC
-
 Homo sapiens
6.3.2.51 CoaB
-
 Homo sapiens
6.3.2.51 PPC synthetase
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.51 ATP
-
 Homo sapiens