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Literature summary extracted from

  • Kupke, T.
    Active-site residues and amino acid specificity of the bacterial 4'-phosphopantothenoylcysteine synthetase CoaB (2004), Eur. J. Biochem., 271, 163-172.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.51 expressed in Escherichia coli M15 cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.3.2.5 A275T temperature sensitive mutant dfp-1 Escherichia coli
6.3.2.5 N210H used for isolation of reaction intermediate Escherichia coli
6.3.2.5 N210K used for isolation of reaction intermediate Escherichia coli
6.3.2.51 A276V the mutation affects enzyme activity Escherichia coli
6.3.2.51 N210D the mutation affects enzyme activity Escherichia coli
6.3.2.51 R206Q the mutation affects enzyme activity Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.51 ATP + (R)-4'-phosphopantothenate + L-cysteine Escherichia coli
-
 AMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.5 Escherichia coli
-
-
-
6.3.2.51 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.51 HiTrapQ column chromatography and Superdex 200 gel filtration Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.2.5 CTP + (R)-4'-phosphopantothenate + L-cysteine = CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine mechanism Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.51 ATP + (R)-4'-phosphopantothenate + L-cysteine
-
 Escherichia coli AMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?
6.3.2.51 CTP + (R)-4'-phosphopantothenate + L-cysteine
-
 Escherichia coli CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?
6.3.2.51 additional information the wild-type enzyme is not able to couple L-serine, L-alanine or DL-homocysteine to (R)-4'-phosphopantothenate Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.51 4'-phosphopantothenoylcysteine synthetase
-
 Escherichia coli
6.3.2.51 CoaB
-
 Escherichia coli
6.3.2.51 Dfp
-
 Escherichia coli
6.3.2.51 PPC-synthetase
-
 Escherichia coli