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Literature summary extracted from

  • Kwon, O.; Bhattacharyya, D.K.; Meganathan, R.
    Menaquinone (vitamin K2) biosynthesis: overexpression, purification, and properties of o-succinylbenzoyl-coenzyme A synthetase from Escherichia coli (1996), J. Bacteriol., 178, 6778-6781.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.26 Fe2+
-
 Escherichia coli
6.2.1.26 Hg2+
-
 Escherichia coli
6.2.1.26 Mg2+ above 1 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.26 0.016
-
 o-succinylbenzoate
-
 Escherichia coli
6.2.1.26 0.0735
-
 ATP
-
 Escherichia coli
6.2.1.26 0.36
-
 CoA
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.26 Ca2+ 0.5 mM, 1 mM or 5 mM, approximately twofold higher activity than the control Escherichia coli
6.2.1.26 Co2+ at 0.5 mM or 1.0 mM, 4fold increase in activity, 2fold increase in activity at 5 mM Escherichia coli
6.2.1.26 K+ 0.5 mM, 1 mM or 5 mM, approximately twofold higher activity than the control Escherichia coli
6.2.1.26 Mg2+ maximal activation at 1 mM Mg2+, 6fold Escherichia coli
6.2.1.26 Mn2+ at 0.5 mM or 1.0 mM, 4fold increase in activity, 2fold increase in activity at 5 mM Escherichia coli
6.2.1.26 Na+ 0.5 mM, 1 mM or 5 mM, approximately twofold higher activity than the control Escherichia coli
6.2.1.26 Zn2+ 0.5 mM, 1 mM or 5 mM, approximately twofold higher activity than the control Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.26 49000
-
 4 * 49000, SDS-PAGE Escherichia coli
6.2.1.26 185000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.26 ATP + 2-succinylbenzoate + CoA Escherichia coli o-succinylbenzoyl-CoA is an intermediate in menaquinone (vitamin K2) biosynthesis AMP + diphosphate + 2-succinylbenzoyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.26 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.26
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.26 3.2
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.26 ATP + 2-succinylbenzoate + CoA
-
 Escherichia coli AMP + diphosphate + 2-succinylbenzoyl-CoA o-succinylbenzoyl-CoA is a highly unstable compound which spontaneously converts to o-succinylbenzoate-spirolactone in absence of 1,4-dihydroxy-2-naphthoic acid synthase ?
6.2.1.26 ATP + 2-succinylbenzoate + CoA o-succinylbenzoyl-CoA is an intermediate in menaquinone (vitamin K2) biosynthesis Escherichia coli AMP + diphosphate + 2-succinylbenzoyl-CoA
-
 ?
6.2.1.26 additional information no activity with: o-malonylbenzoic acid, benzoylpropionic acid, benzoic acid, o-acetylbenzoic acid, phthalic acid and p-coumaric acid Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.26 tetramer 4 * 49000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.26 30 40
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.26 7.5 8
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.26 6.5 8.5 pH 6.5: 55% of maximal activity, pH 8.5: 80% of maximnal activity Escherichia coli