EC Number | General Stability | Organism |
---|---|---|
6.2.1.30 | purified enzyme is sensitive to repeated freezing | Pseudomonas putida |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.2.1.30 | 5,5'-dithiobis(2-nitrobenzoate) | 1 mM, 92% inhibition | Pseudomonas putida | |
6.2.1.30 | Cu2+ | 1 mM, 97% inhibition | Pseudomonas putida | |
6.2.1.30 | Decanoic acid | - |
Pseudomonas putida | |
6.2.1.30 | Hg2+ | 1 mM, 95% inhibition | Pseudomonas putida | |
6.2.1.30 | n-octylglucoside | - |
Pseudomonas putida | |
6.2.1.30 | NEM | 1 mM, 99% inhibition | Pseudomonas putida | |
6.2.1.30 | Nonanoic acid | - |
Pseudomonas putida | |
6.2.1.30 | Nonidet P-40 | - |
Pseudomonas putida | |
6.2.1.30 | octanoic acid | - |
Pseudomonas putida | |
6.2.1.30 | p-chloromercuribenzoate | 1 mM, 96% inhibition | Pseudomonas putida | |
6.2.1.30 | SDS | - |
Pseudomonas putida | |
6.2.1.30 | Tween 100 | - |
Pseudomonas putida | |
6.2.1.30 | Zn2+ | 1 mM, 95% inhibition | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.30 | 1 | - |
CoA | pH 8.2, 30°C | Pseudomonas putida | |
6.2.1.30 | 9.7 | - |
ATP | pH 8.2, 30°C | Pseudomonas putida | |
6.2.1.30 | 16.5 | - |
phenylacetate | pH 8.2, 30°C | Pseudomonas putida |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.2.1.30 | Mg2+ | required | Pseudomonas putida | |
6.2.1.30 | Mn2+ | 50% of the activation with Mg2+ | Pseudomonas putida | |
6.2.1.30 | additional information | enzyme does not contain metal ions | Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.2.1.30 | 48000 | - |
gel filtration | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.30 | ATP + phenylacetate + CoA | Pseudomonas putida | enzyme is involved in the catabolism of phenylacetic acid | ? | - |
? | |
6.2.1.30 | ATP + phenylacetate + CoA | Pseudomonas putida | specifically induced by phenylacetic acid | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.2.1.30 | Pseudomonas putida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.2.1.30 | - |
Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.2.1.30 | additional information | - |
- |
Pseudomonas putida |
6.2.1.30 | 21 | - |
- |
Pseudomonas putida |
EC Number | Storage Stability | Organism |
---|---|---|
6.2.1.30 | -20°C, crude enzyme is stable in presence of glycerol for 2-3 weeks, more than 50% loss of activity of the purified enzyme | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.30 | ADP + phenylacetate + CoA | 28% of the activity with ATP | Pseudomonas putida | ? | - |
? | |
6.2.1.30 | ATP + acetate + CoA | 85% of the activity with phenylacetate | Pseudomonas putida | AMP + diphosphate + acetyl-CoA | - |
? | |
6.2.1.30 | ATP + butanoate + CoA | 19% of the activity with phenylacetate | Pseudomonas putida | AMP + diphosphate + butanoyl-CoA | - |
? | |
6.2.1.30 | ATP + phenylacetate + CoA | - |
Pseudomonas putida | AMP + diphosphate + phenylacetyl-CoA | - |
? | |
6.2.1.30 | ATP + phenylacetate + CoA | enzyme is involved in the catabolism of phenylacetic acid | Pseudomonas putida | ? | - |
? | |
6.2.1.30 | ATP + phenylacetate + CoA | specifically induced by phenylacetic acid | Pseudomonas putida | ? | - |
? | |
6.2.1.30 | ATP + propanoate + CoA | 47% of the activity with phenylacetate | Pseudomonas putida | AMP + diphosphate + propanoyl-CoA | - |
? | |
6.2.1.30 | CTP + phenylacetate + CoA | 2.8% of the activity with ATP | Pseudomonas putida | CMP + diphosphate + phenylacetyl-CoA | - |
? | |
6.2.1.30 | additional information | no activity with: 3-hydroxyphenylacetic acid, 4-hydroxyphenylacetic acid, 3,4-dihydroxyphenylacetic acid | Pseudomonas putida | ? | - |
? | |
6.2.1.30 | UTP + phenylacetate + CoA | 2.5% of the activity with ATP | Pseudomonas putida | UMP + diphosphate + phenylacetyl-CoA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.2.1.30 | monomer | 1 * 48000, SDS-PAGE | Pseudomonas putida |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.2.1.30 | 30 | - |
- |
Pseudomonas putida |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.2.1.30 | additional information | - |
glycerol, 20% v/v, ATP, MgCl2 or phenylacetic acid protects against thermal inactivation | Pseudomonas putida |
6.2.1.30 | 20 | - |
45 min stable, about 15% loss of activity after 60 min | Pseudomonas putida |
6.2.1.30 | 25 | - |
30 min, about 20% loss of activity | Pseudomonas putida |
6.2.1.30 | 30 | - |
60 min, about 40% loss of activity | Pseudomonas putida |
6.2.1.30 | 37 | - |
60 min, about 75% loss of activity | Pseudomonas putida |
6.2.1.30 | 45 | - |
15 min, about 90% loss of activity | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.2.1.30 | 7 | 8.5 | - |
Pseudomonas putida |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
6.2.1.30 | Pseudomonas putida | isoelectric focusing | - |
5.4 |