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Literature summary extracted from

  • Moran, J.F.; Iturbe-Ormaetxe, I.; Matamoros, M.A.; Rubio, M.C.; Clemente, M.R.; Brewin, N.J.; Becana, M.
    Glutathione and homoglutathione synthetases of legume nodules. Cloning, expression, and subcellular localization (2000), Plant Physiol., 124, 1381-1392.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.3 DNA and amino acid sequence determination Pisum sativum
6.3.2.3 DNA and amino acid sequence determination Phaseolus vulgaris
6.3.2.23
-
 Pisum sativum
6.3.2.23
-
 Phaseolus vulgaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.2.3 cytosol isozyme GSHS2 Pisum sativum 5829
-
6.3.2.3 cytosol isozyme GSHS2 Phaseolus vulgaris 5829
-
6.3.2.3 cytosol of nodule bacteroid and leaf Vigna unguiculata 5829
-
6.3.2.3 mitochondrion isozyme GSHS1 Pisum sativum 5739
-
6.3.2.3 mitochondrion of nodule Vigna unguiculata 5739
-
6.3.2.3 plastid isozyme GSHS2 Phaseolus vulgaris 9536
-
6.3.2.23 cytosol
-
 Pisum sativum 5829
-
6.3.2.23 cytosol
-
 Phaseolus vulgaris 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly Vigna unguiculata
-
 ADP + phosphate + gamma-glutathione
-
 ir
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly Pisum sativum
-
 ADP + phosphate + gamma-glutathione
-
 ir
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly Phaseolus vulgaris
-
 ADP + phosphate + gamma-glutathione
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.3 Phaseolus vulgaris Q9FV26 isozyme GSHS2, strain 3622
-
6.3.2.3 Pisum sativum Q9FV27 isozyme GSHS2
-
6.3.2.3 Pisum sativum Q9FVA2 isozyme GSHS1
-
6.3.2.3 Vigna unguiculata
-
 cowpea, strain 32H1
-
6.3.2.23 Phaseolus vulgaris Q9FV26
-
-
6.3.2.23 Pisum sativum Q9FV27
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
6.3.2.3 additional information isozyme GSHS2 contains no signal peptide Pisum sativum
6.3.2.3 proteolytic modification isozyme GSHS1 precursor contains a signal peptide of about 20 amino acid length, cleavage site is FFSKH-/-IPST Pisum sativum
6.3.2.3 proteolytic modification isozyme GSHS2 precursor contains a signal peptide of about 59 amino acid length, cleavage site is NSAPL-/-AEPD Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.3 leaf
-
 Vigna unguiculata
-
6.3.2.3 leaf
-
 Pisum sativum
-
6.3.2.3 additional information isozyme GSHS2 is not expressed in leaves Pisum sativum
-
6.3.2.3 additional information isozyme GSHS2 is not expressed in leaves Phaseolus vulgaris
-
6.3.2.3 nodule
-
 Pisum sativum
-
6.3.2.3 nodule bacteroid Vigna unguiculata
-
6.3.2.3 nodule bacteroid Phaseolus vulgaris
-
6.3.2.23 root root nodules Pisum sativum
-
6.3.2.23 root root nodules Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.3 additional information
-
-
 Vigna unguiculata
6.3.2.3 additional information
-
-
 Pisum sativum
6.3.2.3 additional information
-
-
 Phaseolus vulgaris
6.3.2.3 0.0006
-
 crude extract and cytosolic fraction of leaves Vigna unguiculata
6.3.2.3 0.0008
-
 mitochondrial fraction of leaves Vigna unguiculata
6.3.2.3 0.0094
-
 bacteroid isolate Phaseolus vulgaris
6.3.2.3 0.0127
-
 bacteroid isolate Vigna unguiculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly
-
 Vigna unguiculata ADP + phosphate + gamma-glutathione
-
 ir
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly
-
 Pisum sativum ADP + phosphate + gamma-glutathione
-
 ir
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly
-
 Phaseolus vulgaris ADP + phosphate + gamma-glutathione
-
 ir
6.3.2.23 ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
-
 Pisum sativum ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine
-
 ?
6.3.2.23 ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
-
 Phaseolus vulgaris ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.3 Glutathione synthetase
-
 Vigna unguiculata
6.3.2.3 Glutathione synthetase
-
 Phaseolus vulgaris
6.3.2.3 Glutathione synthetase
-
 Pisum sativum
6.3.2.3 GSHS
-
 Vigna unguiculata
6.3.2.3 GSHS
-
 Phaseolus vulgaris
6.3.2.3 GSHS
-
 Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.3 ATP
-
 Vigna unguiculata
6.3.2.3 ATP
-
 Pisum sativum
6.3.2.3 ATP
-
 Phaseolus vulgaris

pI Value

EC Number Organism Comment pI Value Maximum pI Value
6.3.2.3 Pisum sativum isozyme GSHS2, isoelectric focusing
-
 5.5
6.3.2.3 Phaseolus vulgaris isozyme GSHS2 precursor, isoelectric focusing 5.9 5.6
6.3.2.3 Pisum sativum isozyme GSHS1 precursor, isoelectric focusing
-
 6.5