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Literature summary extracted from

  • Kuhm, A.E.; Schloemann, M.; Knackmuss, H.J.; Pieper, D.H.
    Purification and characterization of dichloromuconate cycloisomerase from Alcaligenes eutrophus JMP 134 (1990), Biochem. J., 266, 877-883.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.5.1.11 citrate
-
 Cupriavidus necator
5.5.1.11 EDTA 1.3 mM Cupriavidus necator
5.5.1.11 PCMB
-
 Cupriavidus necator
5.5.1.11 phosphate
-
 Cupriavidus necator
5.5.1.11 succinate
-
 Cupriavidus necator

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5.1.11 0.02
-
 2,4-dichloro-cis,cis-muconate
-
 Cupriavidus necator
5.5.1.11 0.128
-
 2-methyl-cis,cis-muconate
-
 Cupriavidus necator
5.5.1.11 0.158
-
 3-methyl-cis,cis-muconate
-
 Cupriavidus necator
5.5.1.11 0.304
-
 3-chloro-cis,cis-muconate
-
 Cupriavidus necator
5.5.1.11 0.502
-
 2-chloro-cis,cis-muconate
-
 Cupriavidus necator
5.5.1.11 4.2
-
 cis,cis-muconate
-
 Cupriavidus necator

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.5.1.11 Mn2+ requirement Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.5.1.11 40000
-
 6 * 40000, SDS-PAGE Cupriavidus necator
5.5.1.11 270000
-
 gel filtration Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.11 Cupriavidus necator
-
 JMP 134
-
5.5.1.11 Cupriavidus necator JMP 134-1
-
 JMP 134
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.11
-
 Cupriavidus necator

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.5.1.11 culture condition:2,4-dichlorophenoxyacetic acid-grown cell
-
 Cupriavidus necator
-
5.5.1.11 culture condition:benzoate-grown cell
-
 Cupriavidus necator
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.5.1.11 80.4
-
-
 Cupriavidus necator

Storage Stability

EC Number Storage Stability Organism
5.5.1.11 -16°C, without MnSO4, 95% loss of activity after 10 days Cupriavidus necator
5.5.1.11 4°C, pH 7.5-8.5, 10-30% loss of activity after 24 h Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.11 2,4-dichloro-cis,cis-muconate
-
 Cupriavidus necator 4-carboxymethylene-3-chlorobut-2-en-4-olide + HCl
-
 ?
5.5.1.11 2,4-dichloro-cis,cis-muconate
-
 Cupriavidus necator JMP 134-1 4-carboxymethylene-3-chlorobut-2-en-4-olide + HCl
-
 ?
5.5.1.11 2-Chloro-cis,cis-muconate
-
 Cupriavidus necator ?
-
 ?
5.5.1.11 2-Chloro-cis,cis-muconate
-
 Cupriavidus necator JMP 134-1 ?
-
 ?
5.5.1.11 2-Methyl-cis,cis-muconate
-
 Cupriavidus necator ?
-
 ?
5.5.1.11 2-Methyl-cis,cis-muconate
-
 Cupriavidus necator JMP 134-1 ?
-
 ?
5.5.1.11 3-chloro-cis,cis-muconate
-
 Cupriavidus necator ?
-
 ?
5.5.1.11 3-Methyl-cis,cis-muconate
-
 Cupriavidus necator ?
-
 ?
5.5.1.11 3-Methyl-cis,cis-muconate
-
 Cupriavidus necator JMP 134-1 ?
-
 ?
5.5.1.11 cis,cis-Muconate
-
 Cupriavidus necator ?
-
 ?
5.5.1.11 cis,cis-Muconate
-
 Cupriavidus necator JMP 134-1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.5.1.11 hexamer 6 * 40000, SDS-PAGE Cupriavidus necator

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5.1.11 10.2
-
 2-methyl-cis,cis-muconate
-
 Cupriavidus necator
5.5.1.11 15.8
-
 2-chloro-cis,cis-muconate
-
 Cupriavidus necator
5.5.1.11 51.5
-
 cis,cis-muconate
-
 Cupriavidus necator
5.5.1.11 54.8
-
 3-methyl-cis,cis-muconate
-
 Cupriavidus necator
5.5.1.11 63.7
-
 2,4-dichloro-cis,cis-muconate
-
 Cupriavidus necator
5.5.1.11 119
-
 3-chloro-cis,cis-muconate
-
 Cupriavidus necator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5.1.11 6
-
-
 Cupriavidus necator

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.5.1.11 5.5 8 above 80% of activity maximum at pH 5.5 and 8 Cupriavidus necator

Cofactor

EC Number Cofactor Comment Organism Structure
5.5.1.11 additional information thiol groups required Cupriavidus necator