Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bisswanger, H.; Kirschner, K.; Cohn, W.; Hager, V.; Hansson, E.
    N-(5-Phosphoribosyl)anthranilate isomerase-indoleglycerol-phosphate synthase. 1. A Substrate analogue binds to two different binding sites on the bifunctional enzyme from Escherichia coli (1979), Biochemistry, 18, 5946-5953.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.24
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.24 additional information
-
 additional information
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.24 46000
-
 indole-3-glycerol-phosphate synthetase/PRA isomerase complex, sedimentation equilibrium Escherichia coli
5.3.1.24 48000
-
 1 * 48000, indole-3-glycerol-phosphate synthetase/PRA isomerase complex, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Escherichia coli involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Escherichia coli W3110 / ATCC 27325 involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.24 Escherichia coli
-
-
-
5.3.1.24 Escherichia coli
-
 bifunctional indole-3-glycerol-phosphate synthetase/PRA isomerase complex
-
5.3.1.24 Escherichia coli W3110 / ATCC 27325
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.24 indole-3-glycerol-phosphate synthetase/PRA isomerase complex Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate
-
 Escherichia coli 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Escherichia coli 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate
-
 Escherichia coli W3110 / ATCC 27325 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Escherichia coli W3110 / ATCC 27325 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.24 monomer 1 * 48000, indole-3-glycerol-phosphate synthetase/PRA isomerase complex, SDS-PAGE Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.3.1.24 6.5 7.5 25°C, 16 h, most stable in potassium phosphate buffer, in presence of 1 mM dithioerythritol Escherichia coli