EC Number | Crystallization (Comment) | Organism |
---|---|---|
5.3.1.24 | - |
Escherichia coli |
EC Number | General Stability | Organism |
---|---|---|
5.3.1.24 | enzyme is resistant to protease attack | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.3.1.24 | reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
Acinetobacter calcoaceticus | |
5.3.1.24 | reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
Bacillus subtilis | |
5.3.1.24 | reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
[Brevibacterium] flavum | |
5.3.1.24 | reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
Escherichia coli | |
5.3.1.24 | reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
Pseudomonas putida | |
5.3.1.24 | reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
5.3.1.24 | reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
Serratia marcescens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.24 | 0.007 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | pH 7.5, 20°C | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.3.1.24 | 49370 | - |
1 * 49370, calculated from the amino acid sequence | Escherichia coli |
5.3.1.24 | 49400 | - |
1 * 49400, indole-3-glycerol-phosphate synthetase/PRA isomerase complex | Salmonella enterica subsp. enterica serovar Typhimurium |
5.3.1.24 | 49400 | - |
1 * 49400, indole-3-glycerol-phosphate synthetase/PRA isomerase complex | Escherichia coli |
5.3.1.24 | 49400 | - |
1 * 49400, indole-3-glycerol-phosphate synthetase/PRA isomerase complex | Serratia marcescens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | Salmonella enterica subsp. enterica serovar Typhimurium | fourth step in tryptophan biosynthesis | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | Bacillus subtilis | fourth step in tryptophan biosynthesis | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | Escherichia coli | fourth step in tryptophan biosynthesis | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | Aspergillus nidulans | fourth step in tryptophan biosynthesis | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | Neurospora crassa | fourth step in tryptophan biosynthesis | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | Pseudomonas putida | fourth step in tryptophan biosynthesis | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | Acinetobacter calcoaceticus | fourth step in tryptophan biosynthesis | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | Serratia marcescens | fourth step in tryptophan biosynthesis | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | [Brevibacterium] flavum | fourth step in tryptophan biosynthesis | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | Salmonella enterica subsp. enterica serovar Typhimurium | involved in tryptophan biosynthesis from chorismate | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | Bacillus subtilis | involved in tryptophan biosynthesis from chorismate | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | Escherichia coli | involved in tryptophan biosynthesis from chorismate | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | Aspergillus nidulans | involved in tryptophan biosynthesis from chorismate | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | Neurospora crassa | involved in tryptophan biosynthesis from chorismate | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | Pseudomonas putida | involved in tryptophan biosynthesis from chorismate | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | Acinetobacter calcoaceticus | involved in tryptophan biosynthesis from chorismate | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | Serratia marcescens | involved in tryptophan biosynthesis from chorismate | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | [Brevibacterium] flavum | involved in tryptophan biosynthesis from chorismate | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | Escherichia coli W3110 / ATCC 27325 | fourth step in tryptophan biosynthesis | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | Escherichia coli W3110 / ATCC 27325 | involved in tryptophan biosynthesis from chorismate | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.1.24 | Acinetobacter calcoaceticus | - |
not associated with indole-3-glycerol-phosphate synthetase | - |
5.3.1.24 | Aspergillus nidulans | - |
multienzyme complex, in which one of the two different subunits carries three catalytic functions, including PRA isomerase and indole-3-glycerol-phosphate synthetase | - |
5.3.1.24 | Bacillus subtilis | - |
not associated with indole-3-glycerol-phosphate synthetase | - |
5.3.1.24 | Escherichia coli | - |
- |
- |
5.3.1.24 | Escherichia coli | - |
bifunctional indole-3-glycerol-phosphate synthetase/PRA isomerase complex | - |
5.3.1.24 | Escherichia coli W3110 / ATCC 27325 | - |
- |
- |
5.3.1.24 | Neurospora crassa | - |
multienzyme complex, in which one of the two different subunits carries three catalytic functions, including PRA isomerase and indole-3-glycerol-phosphate synthetase | - |
5.3.1.24 | Pseudomonas putida | - |
not associated with indole-3-glycerol-phosphate synthetase | - |
5.3.1.24 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
multifunctional indole-3-glycerol-phosphate synthetase/PRA isomerase complex | - |
5.3.1.24 | Serratia marcescens | - |
bifunctional indole-3-glycerol-phosphate synthetase/PRA isomerase complex | - |
5.3.1.24 | [Brevibacterium] flavum | - |
indole-3-glycerol-phosphate synthetase and PRA isomerase form an easily dissociable multienzyme complex | - |
EC Number | Oxidation Stability | Organism |
---|---|---|
5.3.1.24 | very susceptible to autooxidation catalyzed by Cu2+ and other heavy metal ions, oxidation is prevented by EDTA and dithioerythritol | Escherichia coli |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.3.1.24 | indole-3-glycerol-phosphate synthetase/PRA isomerase complex | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.3.1.24 | 8.78 | - |
37°C | Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
5.3.1.24 | 4°C, under argon, months, stable | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
ir | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | - |
Bacillus subtilis | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
ir | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | - |
Aspergillus nidulans | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
ir | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | - |
Neurospora crassa | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
ir | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | - |
Pseudomonas putida | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
ir | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | - |
Acinetobacter calcoaceticus | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
ir | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | - |
Serratia marcescens | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
ir | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | - |
[Brevibacterium] flavum | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
ir | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | structure of the indole-3-glycerol-phosphate synthetase/PRA isomerase complex, 2 well-defined functional domains: N-terminal indole-3-glycerol-phosphate synthetase, residues 1-255, and C-terminal PRA isomerase, residues 256-452, with limited noncovalent contacts between the domains, the two reactions are catalyzed independently from each other | Escherichia coli | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
ir | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | fourth step in tryptophan biosynthesis | Salmonella enterica subsp. enterica serovar Typhimurium | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | fourth step in tryptophan biosynthesis | Bacillus subtilis | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | fourth step in tryptophan biosynthesis | Escherichia coli | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | fourth step in tryptophan biosynthesis | Aspergillus nidulans | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | fourth step in tryptophan biosynthesis | Neurospora crassa | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | fourth step in tryptophan biosynthesis | Pseudomonas putida | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | fourth step in tryptophan biosynthesis | Acinetobacter calcoaceticus | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | fourth step in tryptophan biosynthesis | Serratia marcescens | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | fourth step in tryptophan biosynthesis | [Brevibacterium] flavum | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | involved in tryptophan biosynthesis from chorismate | Salmonella enterica subsp. enterica serovar Typhimurium | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | involved in tryptophan biosynthesis from chorismate | Bacillus subtilis | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | involved in tryptophan biosynthesis from chorismate | Escherichia coli | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | involved in tryptophan biosynthesis from chorismate | Aspergillus nidulans | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | involved in tryptophan biosynthesis from chorismate | Neurospora crassa | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | involved in tryptophan biosynthesis from chorismate | Pseudomonas putida | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | involved in tryptophan biosynthesis from chorismate | Acinetobacter calcoaceticus | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | involved in tryptophan biosynthesis from chorismate | Serratia marcescens | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | involved in tryptophan biosynthesis from chorismate | [Brevibacterium] flavum | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | structure of the indole-3-glycerol-phosphate synthetase/PRA isomerase complex, 2 well-defined functional domains: N-terminal indole-3-glycerol-phosphate synthetase, residues 1-255, and C-terminal PRA isomerase, residues 256-452, with limited noncovalent contacts between the domains, the two reactions are catalyzed independently from each other | Escherichia coli W3110 / ATCC 27325 | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
ir | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | fourth step in tryptophan biosynthesis | Escherichia coli W3110 / ATCC 27325 | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | involved in tryptophan biosynthesis from chorismate | Escherichia coli W3110 / ATCC 27325 | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.3.1.24 | monomer | 1 * 49400, indole-3-glycerol-phosphate synthetase/PRA isomerase complex | Salmonella enterica subsp. enterica serovar Typhimurium |
5.3.1.24 | monomer | 1 * 49400, indole-3-glycerol-phosphate synthetase/PRA isomerase complex | Escherichia coli |
5.3.1.24 | monomer | 1 * 49400, indole-3-glycerol-phosphate synthetase/PRA isomerase complex | Serratia marcescens |
5.3.1.24 | monomer | 1 * 49370, calculated from the amino acid sequence | Escherichia coli |
5.3.1.24 | monomer | separate monomeric enzyme | Bacillus subtilis |
5.3.1.24 | monomer | separate monomeric enzyme | Pseudomonas putida |
5.3.1.24 | monomer | separate monomeric enzyme | Acinetobacter calcoaceticus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.24 | 39 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | pH 7.5, 20°C | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.24 | 8.6 | - |
assay at | Escherichia coli |