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Literature summary extracted from

  • Kirschner, K.; Szadkowski, H.; Jardetzky, T.S.; Hager, V.
    Phosphoribosylanthranilate isomerase-indoleglycerol-phosphate synthase from Escherichia coli (1987), Methods Enzymol., 142, 386-397.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.24
-
Escherichia coli

General Stability

EC Number General Stability Organism
5.3.1.24 enzyme is resistant to protease attack Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.24 reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
Acinetobacter calcoaceticus
5.3.1.24 reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
Bacillus subtilis
5.3.1.24 reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
[Brevibacterium] flavum
5.3.1.24 reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
Escherichia coli
5.3.1.24 reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
Pseudomonas putida
5.3.1.24 reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
Salmonella enterica subsp. enterica serovar Typhimurium
5.3.1.24 reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
Serratia marcescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.24 0.007
-
N-(5-phospho-beta-D-ribosyl)anthranilate pH 7.5, 20°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.24 49370
-
1 * 49370, calculated from the amino acid sequence Escherichia coli
5.3.1.24 49400
-
1 * 49400, indole-3-glycerol-phosphate synthetase/PRA isomerase complex Salmonella enterica subsp. enterica serovar Typhimurium
5.3.1.24 49400
-
1 * 49400, indole-3-glycerol-phosphate synthetase/PRA isomerase complex Escherichia coli
5.3.1.24 49400
-
1 * 49400, indole-3-glycerol-phosphate synthetase/PRA isomerase complex Serratia marcescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Salmonella enterica subsp. enterica serovar Typhimurium fourth step in tryptophan biosynthesis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Bacillus subtilis fourth step in tryptophan biosynthesis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Escherichia coli fourth step in tryptophan biosynthesis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Aspergillus nidulans fourth step in tryptophan biosynthesis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Neurospora crassa fourth step in tryptophan biosynthesis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Pseudomonas putida fourth step in tryptophan biosynthesis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Acinetobacter calcoaceticus fourth step in tryptophan biosynthesis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Serratia marcescens fourth step in tryptophan biosynthesis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate [Brevibacterium] flavum fourth step in tryptophan biosynthesis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Salmonella enterica subsp. enterica serovar Typhimurium involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Bacillus subtilis involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Escherichia coli involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Aspergillus nidulans involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Neurospora crassa involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Pseudomonas putida involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Acinetobacter calcoaceticus involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Serratia marcescens involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate [Brevibacterium] flavum involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Escherichia coli W3110 / ATCC 27325 fourth step in tryptophan biosynthesis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Escherichia coli W3110 / ATCC 27325 involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.24 Acinetobacter calcoaceticus
-
not associated with indole-3-glycerol-phosphate synthetase
-
5.3.1.24 Aspergillus nidulans
-
multienzyme complex, in which one of the two different subunits carries three catalytic functions, including PRA isomerase and indole-3-glycerol-phosphate synthetase
-
5.3.1.24 Bacillus subtilis
-
not associated with indole-3-glycerol-phosphate synthetase
-
5.3.1.24 Escherichia coli
-
-
-
5.3.1.24 Escherichia coli
-
bifunctional indole-3-glycerol-phosphate synthetase/PRA isomerase complex
-
5.3.1.24 Escherichia coli W3110 / ATCC 27325
-
-
-
5.3.1.24 Neurospora crassa
-
multienzyme complex, in which one of the two different subunits carries three catalytic functions, including PRA isomerase and indole-3-glycerol-phosphate synthetase
-
5.3.1.24 Pseudomonas putida
-
not associated with indole-3-glycerol-phosphate synthetase
-
5.3.1.24 Salmonella enterica subsp. enterica serovar Typhimurium
-
multifunctional indole-3-glycerol-phosphate synthetase/PRA isomerase complex
-
5.3.1.24 Serratia marcescens
-
bifunctional indole-3-glycerol-phosphate synthetase/PRA isomerase complex
-
5.3.1.24 [Brevibacterium] flavum
-
indole-3-glycerol-phosphate synthetase and PRA isomerase form an easily dissociable multienzyme complex
-

Oxidation Stability

EC Number Oxidation Stability Organism
5.3.1.24 very susceptible to autooxidation catalyzed by Cu2+ and other heavy metal ions, oxidation is prevented by EDTA and dithioerythritol Escherichia coli

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.24 indole-3-glycerol-phosphate synthetase/PRA isomerase complex Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.24 8.78
-
37°C Escherichia coli

Storage Stability

EC Number Storage Stability Organism
5.3.1.24 4°C, under argon, months, stable Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate
-
Salmonella enterica subsp. enterica serovar Typhimurium 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
ir
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate
-
Bacillus subtilis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
ir
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate
-
Aspergillus nidulans 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
ir
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate
-
Neurospora crassa 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
ir
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate
-
Pseudomonas putida 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
ir
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate
-
Acinetobacter calcoaceticus 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
ir
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate
-
Serratia marcescens 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
ir
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate
-
[Brevibacterium] flavum 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
ir
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate structure of the indole-3-glycerol-phosphate synthetase/PRA isomerase complex, 2 well-defined functional domains: N-terminal indole-3-glycerol-phosphate synthetase, residues 1-255, and C-terminal PRA isomerase, residues 256-452, with limited noncovalent contacts between the domains, the two reactions are catalyzed independently from each other Escherichia coli 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
ir
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate fourth step in tryptophan biosynthesis Salmonella enterica subsp. enterica serovar Typhimurium 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate fourth step in tryptophan biosynthesis Bacillus subtilis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate fourth step in tryptophan biosynthesis Escherichia coli 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate fourth step in tryptophan biosynthesis Aspergillus nidulans 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate fourth step in tryptophan biosynthesis Neurospora crassa 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate fourth step in tryptophan biosynthesis Pseudomonas putida 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate fourth step in tryptophan biosynthesis Acinetobacter calcoaceticus 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate fourth step in tryptophan biosynthesis Serratia marcescens 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate fourth step in tryptophan biosynthesis [Brevibacterium] flavum 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Salmonella enterica subsp. enterica serovar Typhimurium 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Bacillus subtilis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Escherichia coli 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Aspergillus nidulans 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Neurospora crassa 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Pseudomonas putida 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Acinetobacter calcoaceticus 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Serratia marcescens 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate [Brevibacterium] flavum 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate structure of the indole-3-glycerol-phosphate synthetase/PRA isomerase complex, 2 well-defined functional domains: N-terminal indole-3-glycerol-phosphate synthetase, residues 1-255, and C-terminal PRA isomerase, residues 256-452, with limited noncovalent contacts between the domains, the two reactions are catalyzed independently from each other Escherichia coli W3110 / ATCC 27325 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
ir
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate fourth step in tryptophan biosynthesis Escherichia coli W3110 / ATCC 27325 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Escherichia coli W3110 / ATCC 27325 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?

Subunits

EC Number Subunits Comment Organism
5.3.1.24 monomer 1 * 49400, indole-3-glycerol-phosphate synthetase/PRA isomerase complex Salmonella enterica subsp. enterica serovar Typhimurium
5.3.1.24 monomer 1 * 49400, indole-3-glycerol-phosphate synthetase/PRA isomerase complex Escherichia coli
5.3.1.24 monomer 1 * 49400, indole-3-glycerol-phosphate synthetase/PRA isomerase complex Serratia marcescens
5.3.1.24 monomer 1 * 49370, calculated from the amino acid sequence Escherichia coli
5.3.1.24 monomer separate monomeric enzyme Bacillus subtilis
5.3.1.24 monomer separate monomeric enzyme Pseudomonas putida
5.3.1.24 monomer separate monomeric enzyme Acinetobacter calcoaceticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.24 39
-
N-(5-phospho-beta-D-ribosyl)anthranilate pH 7.5, 20°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.24 8.6
-
assay at Escherichia coli