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Literature summary extracted from

  • Green, C.; Kammen, H.; Penhoet, E.
    Purification and properties of a mammalian tRNA pseudouridine synthase (1982), J. Biol. Chem., 257, 3045-3052.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.99.12 additional information a thiol is required for maximum activity Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.12 Calf thymus DNA native and denatured, about 66% residual activity Bos taurus
5.4.99.12 poly(rA)n 71% residual acivity Bos taurus
5.4.99.12 poly(rC)n 93% residual acivity Bos taurus
5.4.99.12 poly(rG)n 3.2% residual acivity Bos taurus
5.4.99.12 poly(rI)n 11.9% residual acivity Bos taurus
5.4.99.12 poly(rU)n 44% residual acivity Bos taurus
5.4.99.12 tRNA activity is inhibited by single tRNAs or bulk tRNA from all sources tested, and by ribosomal RNA Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.99.12 additional information a monovalent cation is required for maximum activity Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.12 Bos taurus
-
 steer
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.4.99.12 thymus
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.12 Salmonella typhimurium HisT- tRNALeu tRNAPhe2 from mutant HisT- strain defective for tRNA pseudouridine synthase I that forms pseudouridine in the 3'-side of the anticodon region of approximately half of the cellular tRNAs Bos taurus ?
-
 ?
5.4.99.12 Salmonella typhimurium HisT- tRNAPhe2 uridine39 tRNAPhe2 from mutant HisT- strain defective for tRNA pseudouridine synthase I that forms pseudouridine in the 3’ side of the anticodon region of approximately half of the cellular tRNAs Bos taurus Salmonella typhimurium HisT- tRNAPhe2 pseudouridine39 specific modification of uridine39 ?
5.4.99.12 Salmonelly typhimurium HisT- tRNATyr tRNAPhe2 from mutant HisT- strain defective for tRNA pseudouridine synthase I that forms pseudouridine in the 3'-side of the anticodon region of approximately half of the cellular tRNAs Bos taurus ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
5.4.99.12 additional information no cofactors required Bos taurus