EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.82 | additional information | enzyme is induced by growth on D-xylose, but not by D-gluconate, D-glucose, L-arabinose or D-fucose | Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.82 | EDTA | - |
Pseudomonas sp. | |
4.2.1.82 | additional information | not inhibited by thiols, iodoacetic acid, p-mercuribenzoate or N-ethylmaleimide, all at 2 mM | Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.82 | 2.77 | - |
D-gluconate | - |
Pseudomonas sp. | |
4.2.1.82 | 2.98 | - |
D-xylonate | - |
Pseudomonas sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.82 | FeSO4 | addition of 5 mM to the EDTA-inhibited enzyme results in 75% of the original activity | Pseudomonas sp. | |
4.2.1.82 | MgCl2 | addition of 5 mM to the EDTA-inhibited enzyme results in 100% of the original activity | Pseudomonas sp. | |
4.2.1.82 | MgSO4 | addition of 5 mM to the EDTA-inhibited enzyme results in 100% of the original activity | Pseudomonas sp. | |
4.2.1.82 | Mn2+ | addition of 5 mM to the EDTA-inhibited enzyme results in 13% of the original activity | Pseudomonas sp. | |
4.2.1.82 | additional information | absolute requirement for a divalent cation, no reactivation of EDTA-inhibited enzyme by CuSO4, CoCl2, ZnSO4 and NiCl2, all at 5 mM | Pseudomonas sp. | |
4.2.1.82 | PbCl2 | addition of 5 mM to the EDTA-inhibited enzyme results in 50% of the original activity | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.82 | additional information | Pseudomonas sp. | enzyme functions in the metabolism of D-xylose, but is probably not operative in glucose/gluconate metabolism | ? | - |
? | |
4.2.1.82 | additional information | Pseudomonas sp. MSU-1 | enzyme functions in the metabolism of D-xylose, but is probably not operative in glucose/gluconate metabolism | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.82 | Pseudomonas sp. | - |
- |
- |
4.2.1.82 | Pseudomonas sp. MSU-1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.82 | partial, 21fold | Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.82 | 1.38 | - |
- |
Pseudomonas sp. |
EC Number | Storage Stability | Organism |
---|---|---|
4.2.1.82 | frozen state, several months, stable | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.82 | D-gluconate | at 55% of the rate with D-xylonate | Pseudomonas sp. | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
4.2.1.82 | D-gluconate | at 55% of the rate with D-xylonate | Pseudomonas sp. MSU-1 | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
4.2.1.82 | D-xylonate | - |
Pseudomonas sp. | 2-dehydro-3-deoxy-D-arabinonate + H2O | - |
? | |
4.2.1.82 | D-xylonate | - |
Pseudomonas sp. MSU-1 | 2-dehydro-3-deoxy-D-arabinonate + H2O | - |
? | |
4.2.1.82 | additional information | not: D- and L-arabonate, D- and L-fuconate, 6-iodo-6-deoxygalactonate, D- and L-mannonate, D- and L-galactonate, L-gluconate, D-ribonate, D-glucuronate, D-galacturonate, D-galactarate | Pseudomonas sp. | ? | - |
? | |
4.2.1.82 | additional information | enzyme functions in the metabolism of D-xylose, but is probably not operative in glucose/gluconate metabolism | Pseudomonas sp. | ? | - |
? | |
4.2.1.82 | additional information | not: D- and L-arabonate, D- and L-fuconate, 6-iodo-6-deoxygalactonate, D- and L-mannonate, D- and L-galactonate, L-gluconate, D-ribonate, D-glucuronate, D-galacturonate, D-galactarate | Pseudomonas sp. MSU-1 | ? | - |
? | |
4.2.1.82 | additional information | enzyme functions in the metabolism of D-xylose, but is probably not operative in glucose/gluconate metabolism | Pseudomonas sp. MSU-1 | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.82 | 25 | 30 | assay at | Pseudomonas sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.82 | 60 | - |
half-life: 14 min | Pseudomonas sp. |
4.2.1.82 | 70 | - |
half-life: 2 min | Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.82 | 8 | - |
in 50 mM Bicine | Pseudomonas sp. |