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Literature summary extracted from

  • Dahms, A.S.; Donald, A.
    D-xylo-aldonate dehydratase (1982), Methods Enzymol., 90, 302-305.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.82 additional information enzyme is induced by growth on D-xylose, but not by D-gluconate, D-glucose, L-arabinose or D-fucose Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.82 EDTA
-
Pseudomonas sp.
4.2.1.82 additional information not inhibited by thiols, iodoacetic acid, p-mercuribenzoate or N-ethylmaleimide, all at 2 mM Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.82 2.77
-
D-gluconate
-
Pseudomonas sp.
4.2.1.82 2.98
-
D-xylonate
-
Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.82 FeSO4 addition of 5 mM to the EDTA-inhibited enzyme results in 75% of the original activity Pseudomonas sp.
4.2.1.82 MgCl2 addition of 5 mM to the EDTA-inhibited enzyme results in 100% of the original activity Pseudomonas sp.
4.2.1.82 MgSO4 addition of 5 mM to the EDTA-inhibited enzyme results in 100% of the original activity Pseudomonas sp.
4.2.1.82 Mn2+ addition of 5 mM to the EDTA-inhibited enzyme results in 13% of the original activity Pseudomonas sp.
4.2.1.82 additional information absolute requirement for a divalent cation, no reactivation of EDTA-inhibited enzyme by CuSO4, CoCl2, ZnSO4 and NiCl2, all at 5 mM Pseudomonas sp.
4.2.1.82 PbCl2 addition of 5 mM to the EDTA-inhibited enzyme results in 50% of the original activity Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.82 additional information Pseudomonas sp. enzyme functions in the metabolism of D-xylose, but is probably not operative in glucose/gluconate metabolism ?
-
?
4.2.1.82 additional information Pseudomonas sp. MSU-1 enzyme functions in the metabolism of D-xylose, but is probably not operative in glucose/gluconate metabolism ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.82 Pseudomonas sp.
-
-
-
4.2.1.82 Pseudomonas sp. MSU-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.82 partial, 21fold Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.82 1.38
-
-
Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
4.2.1.82 frozen state, several months, stable Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.82 D-gluconate at 55% of the rate with D-xylonate Pseudomonas sp. 2-dehydro-3-deoxy-D-gluconate + H2O
-
?
4.2.1.82 D-gluconate at 55% of the rate with D-xylonate Pseudomonas sp. MSU-1 2-dehydro-3-deoxy-D-gluconate + H2O
-
?
4.2.1.82 D-xylonate
-
Pseudomonas sp. 2-dehydro-3-deoxy-D-arabinonate + H2O
-
?
4.2.1.82 D-xylonate
-
Pseudomonas sp. MSU-1 2-dehydro-3-deoxy-D-arabinonate + H2O
-
?
4.2.1.82 additional information not: D- and L-arabonate, D- and L-fuconate, 6-iodo-6-deoxygalactonate, D- and L-mannonate, D- and L-galactonate, L-gluconate, D-ribonate, D-glucuronate, D-galacturonate, D-galactarate Pseudomonas sp. ?
-
?
4.2.1.82 additional information enzyme functions in the metabolism of D-xylose, but is probably not operative in glucose/gluconate metabolism Pseudomonas sp. ?
-
?
4.2.1.82 additional information not: D- and L-arabonate, D- and L-fuconate, 6-iodo-6-deoxygalactonate, D- and L-mannonate, D- and L-galactonate, L-gluconate, D-ribonate, D-glucuronate, D-galacturonate, D-galactarate Pseudomonas sp. MSU-1 ?
-
?
4.2.1.82 additional information enzyme functions in the metabolism of D-xylose, but is probably not operative in glucose/gluconate metabolism Pseudomonas sp. MSU-1 ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.82 25 30 assay at Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.82 60
-
half-life: 14 min Pseudomonas sp.
4.2.1.82 70
-
half-life: 2 min Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.82 8
-
in 50 mM Bicine Pseudomonas sp.