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Literature summary extracted from

  • Shulka, P.S.; Mahadevan, S.
    Indoleacetaldoxime hydro-lyase. II. Purification and properties (1968), Arch. Biochem. Biophys., 125, 873-883.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.8.1.3 glutathione activates Fusarium fujikuroi

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.8.1.3 2,3-Dimercaptopropanol
-
 Fusarium fujikuroi
4.8.1.3 2-mercaptoethanol
-
 Fusarium fujikuroi
4.8.1.3 8-hydroxyquinoline inhibition is partly reversed by ferric citrate, ascorbic acid and dehydroascorbic acid Fusarium fujikuroi
4.8.1.3 Ag+ 1 mM, complete inactivation Fusarium fujikuroi
4.8.1.3 Al3+ 1 mM, complete inactivation Fusarium fujikuroi
4.8.1.3 Benzaldoxime
-
 Fusarium fujikuroi
4.8.1.3 Cu2+ 1 mM, complete inactivation Fusarium fujikuroi
4.8.1.3 Cys
-
 Fusarium fujikuroi
4.8.1.3 Diethyl dithiocarbamate
-
 Fusarium fujikuroi
4.8.1.3 Hg2+ 1 mM, complete inactivation Fusarium fujikuroi
4.8.1.3 KCN activates at 0.001-0.1 mM, inhibits at 1-10 mM Fusarium fujikuroi
4.8.1.3 Mandelaldoxime
-
 Fusarium fujikuroi
4.8.1.3 Mercaptoacetic acid weak Fusarium fujikuroi
4.8.1.3 Mo5+ 1 mM, complete inactivation Fusarium fujikuroi
4.8.1.3 NaBH4 inhibition is partly reversed by pyridoxal-5'-phosphate or dehydroascorbic acid Fusarium fujikuroi
4.8.1.3 NEM
-
 Fusarium fujikuroi
4.8.1.3 p-hydroxymercuribenzoate
-
 Fusarium fujikuroi
4.8.1.3 phenylacetaldoxime competitive Fusarium fujikuroi
4.8.1.3 Phenylpropionaldoxime
-
 Fusarium fujikuroi
4.8.1.3 Zn2+ 1 mM, complete inactivation Fusarium fujikuroi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.8.1.3 0.17
-
 3-indoleacetaldoxime
-
 Fusarium fujikuroi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.8.1.3 Fe3+ Ferric citrate promotes activity Fusarium fujikuroi
4.8.1.3 KCN activates at 0.001-0.1 mM, inhibits at 1-10 mM Fusarium fujikuroi

Organism

EC Number Organism UniProt Comment Textmining
4.8.1.3 Fusarium fujikuroi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.8.1.3
-
 Fusarium fujikuroi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.8.1.3 additional information
-
-
 Fusarium fujikuroi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.8.1.3 3-Indoleacetaldoxime
-
 Fusarium fujikuroi 3-Indoleacetonitrile
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8.1.3 7
-
-
 Fusarium fujikuroi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.8.1.3 6 9 pH 6.0: about 60% of maximal activity, pH 9.0: about 35% of maximal activity Fusarium fujikuroi

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.8.1.3 6 7 maximally stable Fusarium fujikuroi

Cofactor

EC Number Cofactor Comment Organism Structure
4.8.1.3 pyridoxal 5'-phosphate required Fusarium fujikuroi