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Literature summary extracted from

  • Rashid, M.B.; Russell, M.; Mensa-Wilmot, K.
    Roles of Gln81 and Cys80 in catalysis by glycosylphosphatidylinositol-phospholipase C from Trypanosoma brucei (1999), Eur. J. Biochem., 264, 914-920.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.14 C184A activity is not effected Trypanosoma brucei
4.6.1.14 C269-270-273S 33% activity of wild-type enzyme Trypanosoma brucei
4.6.1.14 C80A 33% activity of wild-type enzyme, resistant to p-chloromercuriphenylsulfonic acid Trypanosoma brucei
4.6.1.14 C80F enzyme is inactive Trypanosoma brucei
4.6.1.14 C80T 33% activity of wild-type enzyme, resistant to p-chloromercuriphenylsulfonic acid Trypanosoma brucei
4.6.1.14 Q81A enzyme is inactive Trypanosoma brucei
4.6.1.14 Q81E enzyme is inactive Trypanosoma brucei
4.6.1.14 Q81G enzyme is inactive Trypanosoma brucei
4.6.1.14 Q81K enzyme is inactive Trypanosoma brucei
4.6.1.14 Q81L enzyme is inactive Trypanosoma brucei
4.6.1.14 Q81N specific activity is 500fold decreased Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.14 0.0003
-
 myristate-labeled variant-surface-glycoprotein 37°C, pH 8, 50 mM sodium phosphate, Q81N Trypanosoma brucei
4.6.1.14 0.0005
-
 myristate-labeled variant-surface-glycoprotein 37°C, pH 8, 50 mM sodium phosphate, wild-type Trypanosoma brucei
4.6.1.14 0.0011
-
 myristate-labeled variant-surface-glycoprotein 37°C, pH 8, wild-type Trypanosoma brucei
4.6.1.14 0.0018
-
 myristate-labeled variant-surface-glycoprotein 37°C, pH 8, C80A Trypanosoma brucei
4.6.1.14 0.0021
-
 myristate-labeled variant-surface-glycoprotein 37°C, pH 8, C80T Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.14 Trypanosoma brucei
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6.1.14 2000
-
 Q81E Trypanosoma brucei
4.6.1.14 9000
-
 Q81N Trypanosoma brucei
4.6.1.14 280000
-
 C80A Trypanosoma brucei
4.6.1.14 1100000
-
 wild-type Trypanosoma brucei
4.6.1.14 3500000
-
 C80T Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.14 myristate-labeled variant-surface-glycoprotein
-
 Trypanosoma brucei ?
-
 ?
4.6.1.14 variant-surface-glycoprotein
-
 Trypanosoma brucei 1,2-didecanoylglycerol + soluble variant-surface-glycoprotein
-
 ?