Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Carrington, M.; Carnall, N.; Crow, M.S.; Gaud, A.; Redpath, M.B.; Wasunna, C.L.; Webb, H.
    The properties and function of the glycosylphosphatidylinositol-phospholipase C in Trypanosoma brucei (1998), Mol. Biochem. Parasitol., 91, 153-164.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.14 expression of the PLC elimination construct in Trypanosoma brucei Trypanosoma brucei

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.14 C184S fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid Trypanosoma brucei
4.6.1.14 C24S fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid Trypanosoma brucei
4.6.1.14 C269S fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid Trypanosoma brucei
4.6.1.14 C270S fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid Trypanosoma brucei
4.6.1.14 C273S fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid Trypanosoma brucei
4.6.1.14 C332S fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid Trypanosoma brucei
4.6.1.14 C347S fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid Trypanosoma brucei
4.6.1.14 C80S fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid Trypanosoma brucei
4.6.1.14 H34Q enzyme is totally inactive Trypanosoma brucei
4.6.1.14 additional information PLC elimination construct, which yielded in a PLC null-mutant Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.14 0.0008
-
 Acetylcholinesterase
-
 Trypanosoma brucei
4.6.1.14 0.037
-
 phosphatidylinositol
-
 Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.14 Trypanosoma brucei
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.14 acetylcholinesterase
-
 Trypanosoma brucei soluble acetylcholinesterase + 1,2-diacylglycerol
-
 ?
4.6.1.14 phosphatidylinositol
-
 Trypanosoma brucei inositol 1,2-cyclic phosphate + inositol phosphate
-
 ?
4.6.1.14 variant-surface-glycoprotein
-
 Trypanosoma brucei 1,2-didecanoylglycerol + soluble variant-surface-glycoprotein
-
 ?