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Literature summary extracted from

  • Vuilleumier, S.; Leisinger, T.
    Protein engineering studies of dichloromethane dehalogenase/glutathione S-transferase from Methylophilus sp. strain DM11. Ser12 but not Tyr6 is required for enzyme activity (1996), Eur. J. Biochem., 239, 410-417.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.5.1.3 glutathione
-
 Methylophilus sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.5.1.3
-
 Methylophilus sp.

Protein Variants

EC Number Protein Variants Comment Organism
4.5.1.3 H8F little effect on the activity, but slower turnover of dibromomethane than wild-type Methylophilus sp.
4.5.1.3 H8L little effect on the activity, but slower turnover of dibromomethane than wild-type Methylophilus sp.
4.5.1.3 H8Y little effect on the activity, but slower turnover of dibromomethane than wild-type Methylophilus sp.
4.5.1.3 S12A drastically reduced activity Methylophilus sp.
4.5.1.3 S12T drastically reduced activity Methylophilus sp.
4.5.1.3 Y6A little effect on the activity Methylophilus sp.
4.5.1.3 Y6F little effect on the activity Methylophilus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.5.1.3 0.0005
-
 Dibromomethane 30°C, pH 8.5, Y6A Methylophilus sp.
4.5.1.3 0.001
-
 Dibromomethane 30°C, pH 8.5, H8F Methylophilus sp.
4.5.1.3 0.0029
-
 Dibromomethane 30°C, pH 8.5, Y6F Methylophilus sp.
4.5.1.3 0.0032
-
 Dibromomethane 30°C, pH 8.5, H8Y Methylophilus sp.
4.5.1.3 0.0035
-
 Dibromomethane 30°C, pH 8.5, wild-type Methylophilus sp.
4.5.1.3 0.032
-
 Dichloromethane 30°C, pH 8.5, Y6F Methylophilus sp.
4.5.1.3 0.034
-
 Dichloromethane 30°C, pH 8.5, Y6A Methylophilus sp.
4.5.1.3 0.049
-
 Dichloromethane 30°C, pH 8.5, H8F Methylophilus sp.
4.5.1.3 0.055
-
 Dichloromethane 30°C, pH 8.5, H8Y Methylophilus sp.
4.5.1.3 0.059
-
 Dichloromethane 30°C, pH 8.5, wild-type Methylophilus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.5.1.3 dichloromethane + H2O Methylophilus sp.
-
 formaldehyde + Cl-
-
 ?
4.5.1.3 dichloromethane + H2O Methylophilus sp. DM11
-
 formaldehyde + Cl-
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.5.1.3 Methylophilus sp. P43387
-
-
4.5.1.3 Methylophilus sp. DM11 P43387
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.5.1.3 strain DM11 Methylophilus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.5.1.3 dibromomethane + H2O
-
 Methylophilus sp. formaldehyde + Br-
-
 ?
4.5.1.3 dibromomethane + H2O
-
 Methylophilus sp. DM11 formaldehyde + Br-
-
 ?
4.5.1.3 dichloromethane + H2O
-
 Methylophilus sp. formaldehyde + Cl-
-
 ?
4.5.1.3 dichloromethane + H2O
-
 Methylophilus sp. DM11 formaldehyde + Cl-
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.5.1.3 2.3
-
 Dichloromethane 30°C, pH 8.5, Y6A Methylophilus sp.
4.5.1.3 2.6
-
 Dichloromethane 30°C, pH 8.5, Y6F Methylophilus sp.
4.5.1.3 3
-
 Dichloromethane 30°C, pH 8.5, H8Y Methylophilus sp.
4.5.1.3 3
-
 Dibromomethane 30°C, pH 8.5, H8Y Methylophilus sp.
4.5.1.3 3.3
-
 Dichloromethane 30°C, pH 8.5, wild-type Methylophilus sp.
4.5.1.3 3.4
-
 Dibromomethane 30°C, pH 8.5, H8F Methylophilus sp.
4.5.1.3 3.6
-
 Dichloromethane 30°C, pH 8.5, H8F Methylophilus sp.
4.5.1.3 4.4
-
 Dibromomethane 30°C, pH 8.5, Y6A Methylophilus sp.
4.5.1.3 5.5
-
 Dibromomethane 30°C, pH 8.5, wild-type Methylophilus sp.
4.5.1.3 5.6
-
 Dibromomethane 30°C, pH 8.5, Y6F Methylophilus sp.