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Literature summary extracted from

  • Scholtz, R.; Wackett, L.P.; Egli, C.; Cook, A.M.; Leisinger, T.
    Dichloromethane dehalogenase with improved catalytic activity isolated from a fast-growing dichloromethane-utilizing bacterium (1988), J. Bacteriol., 170, 5698-5704.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.5.1.3 glutathione required Hyphomicrobium sp.
4.5.1.3 glutathione required Methylorubrum extorquens
4.5.1.3 glutathione required Methylophilus sp.
4.5.1.3 glutathione Km: 0.32 mM Hyphomicrobium sp.
4.5.1.3 glutathione KM: 0.062 mM Methylorubrum extorquens
4.5.1.3 glutathione KM: 0.083 mM Methylophilus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.5.1.3 0.02
-
 Dichloromethane 30°C, pH 8 Methylorubrum extorquens
4.5.1.3 0.03
-
 Dichloromethane 30°C, pH 8 Hyphomicrobium sp.
4.5.1.3 0.051
-
 Dichloromethane 30°C, pH 8 Methylophilus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.5.1.3 33000
-
 x * 33000, SDS-PAGE Hyphomicrobium sp.
4.5.1.3 34000
-
 x * 34000, SDS-PAGE Methylophilus sp.
4.5.1.3 35000
-
 x * 35000, SDS-PAGE Methylorubrum extorquens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.5.1.3 dichloromethane + H2O Hyphomicrobium sp.
-
 formaldehyde + Cl-
-
 ?
4.5.1.3 dichloromethane + H2O Methylorubrum extorquens
-
 formaldehyde + Cl-
-
 ?
4.5.1.3 dichloromethane + H2O Methylophilus sp.
-
 formaldehyde + Cl-
-
 ?
4.5.1.3 dichloromethane + H2O Methylophilus sp. DM11
-
 formaldehyde + Cl-
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.5.1.3 Hyphomicrobium sp.
-
-
-
4.5.1.3 Methylophilus sp. P43387
-
-
4.5.1.3 Methylophilus sp. DM11 P43387
-
-
4.5.1.3 Methylorubrum extorquens P21161
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.5.1.3 strain DM11 Methylophilus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.5.1.3 dichloromethane + H2O
-
 Hyphomicrobium sp. formaldehyde + Cl-
-
 ?
4.5.1.3 dichloromethane + H2O
-
 Methylorubrum extorquens formaldehyde + Cl-
-
 ?
4.5.1.3 dichloromethane + H2O
-
 Methylophilus sp. formaldehyde + Cl-
-
 ?
4.5.1.3 dichloromethane + H2O
-
 Methylophilus sp. DM11 formaldehyde + Cl-
-
 ?

Subunits

EC Number Subunits Comment Organism
4.5.1.3 ? x * 35000, SDS-PAGE Methylorubrum extorquens
4.5.1.3 ? x * 34000, SDS-PAGE Methylophilus sp.
4.5.1.3 ? x * 33000, SDS-PAGE Hyphomicrobium sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.5.1.3 0.55
-
 Dichloromethane 30°C, pH 8 Hyphomicrobium sp.
4.5.1.3 0.87
-
 Dichloromethane 30°C, pH 8.2 Methylorubrum extorquens
4.5.1.3 3.28
-
 Dichloromethane 30°C, pH 8.2 Methylophilus sp.