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Literature summary extracted from

  • Leisinger, T.; Kohler-Staub, D.
    Dichloromethane dehalogenase from Hyphomicobium DM2 (1990), Methods Enzymol., 188, 355-361.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.5.1.3 glutathione required Hyphomicrobium sp.
4.5.1.3 glutathione Km: 0.32 mM Hyphomicrobium sp.
4.5.1.3 glutathione sulfhydryl compounds such as 2-mercaptoethanol, DTT or cysteine cannot substitute for reduced glutathione Hyphomicrobium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.5.1.3 1,2-Dichloroethane
-
 Hyphomicrobium sp.
4.5.1.3 1-Chloropropane
-
 Hyphomicrobium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.5.1.3 0.003
-
 Diiodomethane 30°C, pH 8.2 Hyphomicrobium sp.
4.5.1.3 0.013
-
 Dibromomethane 30°C, pH 8.2 Hyphomicrobium sp.
4.5.1.3 0.015
-
 CH2BrCl 30°C, pH 8.2 Hyphomicrobium sp.
4.5.1.3 0.03
-
 Dichloromethane 30°C, pH 8.2 Hyphomicrobium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.5.1.3 33000
-
 alpha6, 6 * 33000, SDS-PAGE Hyphomicrobium sp.
4.5.1.3 195000
-
 gel filtration Hyphomicrobium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.5.1.3 dichloromethane + H2O Hyphomicrobium sp.
-
 formaldehyde + Cl-
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.5.1.3 Hyphomicrobium sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.5.1.3 strain DM2 Hyphomicrobium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.5.1.3 1.041
-
 hydroxylapatite eluate Hyphomicrobium sp.

Storage Stability

EC Number Storage Stability Organism
4.5.1.3 -20°C, protein concentration 4 mg/ml, 100 mM potassium phosphate, pH 7.5, 2.5 mM DTT, 0.1 mM EDTA, 25% v/v glycerol, stable for 6 months Hyphomicrobium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.5.1.3 CH2BrCl + H2O
-
 Hyphomicrobium sp. formaldehyde + Br- + Cl-
-
 ?
4.5.1.3 dibromomethane + H2O
-
 Hyphomicrobium sp. formaldehyde + Br-
-
 ?
4.5.1.3 dichloromethane + H2O
-
 Hyphomicrobium sp. formaldehyde + Cl-
-
 ?
4.5.1.3 diiodomethane + H2O
-
 Hyphomicrobium sp. formaldehyde + I-
-
 ?

Subunits

EC Number Subunits Comment Organism
4.5.1.3 hexamer alpha6, 6 * 33000, SDS-PAGE Hyphomicrobium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.5.1.3 30
-
 assay at Hyphomicrobium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.5.1.3 22
-
 6 days, 50% loss of activity Hyphomicrobium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5.1.3 8.5
-
-
 Hyphomicrobium sp.