Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mihara, H.; Maeda, M.; Fujii, T.; Kurihara, T.; Hata, Y.; Esaki, N.
    A nifS-like gene, csdB, encodes an Escherichia coli counterpart of mammalian selenocysteine lyase. Gene cloning, purification, characterization and preliminary X-ray crystallographic studies (1999), J. Biol. Chem., 274, 14768-14772.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.16 expressed in Escherichia coli JM109 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.4.1.16 hanging drop vapor diffusion method Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.16 43000
-
 SDS-PAGE Escherichia coli
4.4.1.16 44440
-
 calculated from amino acid sequence Escherichia coli
4.4.1.16 88000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.16 L-selenocysteine + reduced acceptor Escherichia coli probably functions as selenide delivery system selenide + L-alanine + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.16 Escherichia coli
-
 stain K-12 ICR130
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.16 overexpressed protein Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.16 5.6
-
 purified enzyme, pH 7.5, 37°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.16 L-cysteine sulfinic acid + reduced acceptor approx. 15% of activity with L-selenocysteine Escherichia coli SO2 + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor
-
 Escherichia coli selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor probably functions as selenide delivery system Escherichia coli selenide + L-alanine + acceptor
-
 ?
4.4.1.16 additional information traces of activity with L-cysteine, L-selenocystine, L-cystine and L-aspartic acid Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.16 dimer 2 * 43000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
4.4.1.16 More two other proteins (CDS and IscS) with activity towards L-selenocysteine identified that are much less specific Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.16 6.5 7.5
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.16 pyridoxal 5'-phosphate required Escherichia coli
4.4.1.16 pyridoxal 5'-phosphate 1 mol bound per mol enzyme (subunit) Escherichia coli