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Literature summary extracted from

  • Esaki, N.; Nakamura, T.; Tanaka, H.; Soda, K.
    Selenocysteine lyase, a novel enzyme that specifically acts on selenocysteine (1982), J. Biol. Chem., 257, 4386-4391.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.4.1.16 1% bovine serum albumin, 20% sucrose or 20% glycerol protects against inactivation, highly diluted samples sensitive to freezing Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.16 L-cysteine competitive Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.16 0.83
-
 L-selenocysteine pH 8.5, 37°C Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.16 48000
-
 SDS-PAGE Sus scrofa
4.4.1.16 85000
-
 sedimentation equilibrium centrifugation Sus scrofa
4.4.1.16 93000
-
 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.16 L-selenocysteine + reduced acceptor Cavia porcellus
-
 selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor Mus musculus
-
 selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor Rattus norvegicus
-
 selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor Bos taurus
-
 selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor Oryctolagus cuniculus
-
 selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor Canis lupus familiaris
-
 selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor Felis catus
-
 selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor Platyrrhini
-
 selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor Sus scrofa most likely metabolizes small amounts of selenocysteine in tissue selenide + L-alanine + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.16 Bos taurus
-
-
-
4.4.1.16 Canis lupus familiaris
-
-
-
4.4.1.16 Cavia porcellus
-
-
-
4.4.1.16 Felis catus
-
-
-
4.4.1.16 Mus musculus
-
-
-
4.4.1.16 Oryctolagus cuniculus
-
-
-
4.4.1.16 Platyrrhini
-
-
-
4.4.1.16 Rattus norvegicus
-
-
-
4.4.1.16 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.16
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.16 adrenal gland
-
 Rattus norvegicus
-
4.4.1.16 adrenal gland
-
 Sus scrofa
-
4.4.1.16 brain low activity Rattus norvegicus
-
4.4.1.16 brain low activity Canis lupus familiaris
-
4.4.1.16 heart
-
 Sus scrofa
-
4.4.1.16 heart low activity Rattus norvegicus
-
4.4.1.16 heart low activity Canis lupus familiaris
-
4.4.1.16 kidney highest activity Cavia porcellus
-
4.4.1.16 kidney highest activity Mus musculus
-
4.4.1.16 kidney highest activity Rattus norvegicus
-
4.4.1.16 kidney highest activity Sus scrofa
-
4.4.1.16 kidney highest activity Oryctolagus cuniculus
-
4.4.1.16 kidney highest activity Canis lupus familiaris
-
4.4.1.16 kidney highest activity Felis catus
-
4.4.1.16 liver highest activity Cavia porcellus
-
4.4.1.16 liver highest activity Mus musculus
-
4.4.1.16 liver highest activity Rattus norvegicus
-
4.4.1.16 liver highest activity Sus scrofa
-
4.4.1.16 liver highest activity Bos taurus
-
4.4.1.16 liver highest activity Oryctolagus cuniculus
-
4.4.1.16 liver highest activity Canis lupus familiaris
-
4.4.1.16 liver highest activity Felis catus
-
4.4.1.16 liver highest activity Platyrrhini
-
4.4.1.16 lung
-
 Rattus norvegicus
-
4.4.1.16 lung
-
 Canis lupus familiaris
-
4.4.1.16 additional information no activity in fat and blood Rattus norvegicus
-
4.4.1.16 muscle
-
 Sus scrofa
-
4.4.1.16 muscle low activity Rattus norvegicus
-
4.4.1.16 pancreas low activity Mus musculus
-
4.4.1.16 pancreas low activity Oryctolagus cuniculus
-
4.4.1.16 pancreas high activity Rattus norvegicus
-
4.4.1.16 pancreas high activity Canis lupus familiaris
-
4.4.1.16 spleen
-
 Rattus norvegicus
-
4.4.1.16 spleen
-
 Canis lupus familiaris
-
4.4.1.16 testis low activity Rattus norvegicus
-
4.4.1.16 thymus
-
 Rattus norvegicus
-
4.4.1.16 thymus
-
 Canis lupus familiaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.16 37
-
 purified enzyme, pH 8.5, 37°C Sus scrofa

Storage Stability

EC Number Storage Stability Organism
4.4.1.16 -20°C, 20% sucrose, stable Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.16 L-selenocysteine + reduced acceptor
-
 Cavia porcellus selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor
-
 Mus musculus selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor
-
 Rattus norvegicus selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor
-
 Bos taurus selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor
-
 Oryctolagus cuniculus selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor
-
 Canis lupus familiaris selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor
-
 Felis catus selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor
-
 Platyrrhini selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor
-
 Sus scrofa selenide + L-alanine + acceptor H2Se detected as product ?
4.4.1.16 L-selenocysteine + reduced acceptor most likely metabolizes small amounts of selenocysteine in tissue Sus scrofa selenide + L-alanine + acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.16 dimer 2 * 48000, SDS-PAGE Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.4.1.16 50
-
 50% of activity lost within 30 min Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.16 9
-
-
 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.16 pyridoxal 5'-phosphate required Cavia porcellus
4.4.1.16 pyridoxal 5'-phosphate required Mus musculus
4.4.1.16 pyridoxal 5'-phosphate required Rattus norvegicus
4.4.1.16 pyridoxal 5'-phosphate required Sus scrofa
4.4.1.16 pyridoxal 5'-phosphate required Bos taurus
4.4.1.16 pyridoxal 5'-phosphate required Oryctolagus cuniculus
4.4.1.16 pyridoxal 5'-phosphate required Canis lupus familiaris
4.4.1.16 pyridoxal 5'-phosphate required Felis catus
4.4.1.16 pyridoxal 5'-phosphate required Platyrrhini
4.4.1.16 pyridoxal 5'-phosphate completely inactive when cofactor is removed, can not be replaced by vitamin B6 derivatives Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.4.1.16 1
-
 L-cysteine pH 8.5, 37°C Sus scrofa